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Involvement of CX3CL1/CX3CR1 signaling in spinal long term potentiation.

Bian C, Zhao ZQ, Zhang YQ, Lü N - PLoS ONE (2015)

Bottom Line: Exogenous CX3CL1 significantly potentiated 3-trains TSS-induced LTP in rats.Consistently, spinal LTP of C-fiber-evoked field potentials was also induced by TSS (100 Hz, 1s, 4 trains) in all C57BL/6 wild type (WT) mice.These results suggest that CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 200032, China.

ABSTRACT
The long-term potentiation (LTP) of spinal C-fiber-evoked field potentials is considered as a fundamental mechanism of central sensitization in the spinal cord. Accumulating evidence has showed the contribution of spinal microglia to spinal LTP and pathological pain. As a key signaling of neurons-microglia interactions, the involvement of CX3CL1/CX3CR1 signaling in pathological pain has also been investigated extensively. The present study examined whether CX3CL1/CX3CR1 signaling plays a role in spinal LTP. The results showed that 10-trains tetanic stimulation (100 Hz, 2s) of the sciatic nerve (TSS) produced a significant LTP of C-fiber-evoked field potentials lasting for over 3 h in the rat spinal dorsal horn. Blockade of CX3CL1/CX3CR1 signaling with an anti-CX3CR1 neutralizing antibody (CX3CR1 AB) markedly suppressed TSS-induced LTP. Exogenous CX3CL1 significantly potentiated 3-trains TSS-induced LTP in rats. Consistently, spinal LTP of C-fiber-evoked field potentials was also induced by TSS (100 Hz, 1s, 4 trains) in all C57BL/6 wild type (WT) mice. However, in CX3CR1-/- mice, TSS failed to induce LTP and behavioral hypersensitivity, confirming an essential role of CX3CR1 in spinal LTP induction. Furthermore, blockade of IL-18 or IL-23, the potential downstream factors of CX3CL1/CX3CR1 signaling, with IL-18 BP or anti-IL-23 neutralizing antibody (IL-23 AB), obviously suppressed spinal LTP in rats. These results suggest that CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn.

No MeSH data available.


Related in: MedlinePlus

A representative spinal long-term potentiation (LTP) of C-fiber-evoked field potentials.Spinal LTP of C-fiber-evoked field potentials was induced by 10-trains tetanic stimulation of the sciatic nerve (TSS); conversely, it was not formed in the sham group (no TSS applied). a & b, the representative C-responses (gray area) in TSS group; c & d, the representative C-responses (gray area) in the sham group.
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pone.0118842.g002: A representative spinal long-term potentiation (LTP) of C-fiber-evoked field potentials.Spinal LTP of C-fiber-evoked field potentials was induced by 10-trains tetanic stimulation of the sciatic nerve (TSS); conversely, it was not formed in the sham group (no TSS applied). a & b, the representative C-responses (gray area) in TSS group; c & d, the representative C-responses (gray area) in the sham group.

Mentions: As described in our previous studies [5, 15, 31], tetanic stimulation of the sciatic nerve (TSS) produced a significant long-term potentiation (LTP) of C-fiber-evoked field potentials lasting for over 3 h in the rat spinal dorsal horn. The representative LTP was illustrated in Fig. 2. After 10-trains TSS, the C-fiber-evoked field potential was amplified about 3 folds than that before TSS. In contrast, in the sham group without TSS, no obvious change in C-response was observed (Two-way ANOVA, treatments: F1, 9 = 138.261, p < 0.01) (Fig. 2).


Involvement of CX3CL1/CX3CR1 signaling in spinal long term potentiation.

Bian C, Zhao ZQ, Zhang YQ, Lü N - PLoS ONE (2015)

A representative spinal long-term potentiation (LTP) of C-fiber-evoked field potentials.Spinal LTP of C-fiber-evoked field potentials was induced by 10-trains tetanic stimulation of the sciatic nerve (TSS); conversely, it was not formed in the sham group (no TSS applied). a & b, the representative C-responses (gray area) in TSS group; c & d, the representative C-responses (gray area) in the sham group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358970&req=5

pone.0118842.g002: A representative spinal long-term potentiation (LTP) of C-fiber-evoked field potentials.Spinal LTP of C-fiber-evoked field potentials was induced by 10-trains tetanic stimulation of the sciatic nerve (TSS); conversely, it was not formed in the sham group (no TSS applied). a & b, the representative C-responses (gray area) in TSS group; c & d, the representative C-responses (gray area) in the sham group.
Mentions: As described in our previous studies [5, 15, 31], tetanic stimulation of the sciatic nerve (TSS) produced a significant long-term potentiation (LTP) of C-fiber-evoked field potentials lasting for over 3 h in the rat spinal dorsal horn. The representative LTP was illustrated in Fig. 2. After 10-trains TSS, the C-fiber-evoked field potential was amplified about 3 folds than that before TSS. In contrast, in the sham group without TSS, no obvious change in C-response was observed (Two-way ANOVA, treatments: F1, 9 = 138.261, p < 0.01) (Fig. 2).

Bottom Line: Exogenous CX3CL1 significantly potentiated 3-trains TSS-induced LTP in rats.Consistently, spinal LTP of C-fiber-evoked field potentials was also induced by TSS (100 Hz, 1s, 4 trains) in all C57BL/6 wild type (WT) mice.These results suggest that CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 200032, China.

ABSTRACT
The long-term potentiation (LTP) of spinal C-fiber-evoked field potentials is considered as a fundamental mechanism of central sensitization in the spinal cord. Accumulating evidence has showed the contribution of spinal microglia to spinal LTP and pathological pain. As a key signaling of neurons-microglia interactions, the involvement of CX3CL1/CX3CR1 signaling in pathological pain has also been investigated extensively. The present study examined whether CX3CL1/CX3CR1 signaling plays a role in spinal LTP. The results showed that 10-trains tetanic stimulation (100 Hz, 2s) of the sciatic nerve (TSS) produced a significant LTP of C-fiber-evoked field potentials lasting for over 3 h in the rat spinal dorsal horn. Blockade of CX3CL1/CX3CR1 signaling with an anti-CX3CR1 neutralizing antibody (CX3CR1 AB) markedly suppressed TSS-induced LTP. Exogenous CX3CL1 significantly potentiated 3-trains TSS-induced LTP in rats. Consistently, spinal LTP of C-fiber-evoked field potentials was also induced by TSS (100 Hz, 1s, 4 trains) in all C57BL/6 wild type (WT) mice. However, in CX3CR1-/- mice, TSS failed to induce LTP and behavioral hypersensitivity, confirming an essential role of CX3CR1 in spinal LTP induction. Furthermore, blockade of IL-18 or IL-23, the potential downstream factors of CX3CL1/CX3CR1 signaling, with IL-18 BP or anti-IL-23 neutralizing antibody (IL-23 AB), obviously suppressed spinal LTP in rats. These results suggest that CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn.

No MeSH data available.


Related in: MedlinePlus