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Identification of bacteriology and risk factor analysis of asymptomatic bacterial colonization in pacemaker replacement patients.

Chu XM, Yu H, Sun XX, An Y, Li B, Li XB - PLoS ONE (2015)

Bottom Line: Twenty two (18.6%) patients had positive bacterial culture results, of which 50% had coagulase-negative staphylococcus.Positive bacterial DNA results from pocket tissues and the surface of the devices were 22.0% and 29.7%, respectively.Bacterial culture combined genetic testing could improve the detection rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, the Affiliated Hospital of Qingdao University, Qingdao, 266100, China.

ABSTRACT

Background: Recent researches revealed that asymptomatic bacterial colonization on PMs might be ubiquitous and increase the risk of clinical PM infection. Early diagnosis of patients with asymptomatic bacterial colonization could provide opportunity for targeted preventive measures.

Objective: The present study explores the incidence of bacterial colonization of generator pockets in pacemaker replacement patients without signs of infection, and to analyze risk factors for asymptomatic bacterial colonization.

Methods: From June 2011 to December 2013, 118 patients underwent pacemaker replacement or upgrade. Identification of bacteria was carried out by bacterial culture and 16S rRNA sequencing. Clinical risk characteristics were analyzed.

Results: The total bacterial positive rate was 37.3% (44 cases), and the coagulase-negative Staphylococcus aureus detection rate was the highest. Twenty two (18.6%) patients had positive bacterial culture results, of which 50% had coagulase-negative staphylococcus. The bacterial DNA detection rate was 36.4 % (43 cases). Positive bacterial DNA results from pocket tissues and the surface of the devices were 22.0% and 29.7%, respectively. During follow-up (median, 27.0 months), three patients (6.8%, 3/44) became symptomatic with the same genus of microorganism, S. aureus (n=2) and S. epidermidis (n=1). Multivariable logistic regression analysis showed that history of bacterial infection, use of antibiotics, application of antiplatelet drugs, replacement frequency were independent risk factors for asymptomatic bacterial colonization.

Conclusion: There was a high incidence of asymptomatic bacterial colonization in pacemaker patients with independent risk factors. Bacterial culture combined genetic testing could improve the detection rate.

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Related in: MedlinePlus

Identification of enzyme digestion of 16S rRNA gene recombinant.1, 3 represented blank pGEM-T plasmid; 2, 4 represented enzyme-digested product of 16S rRNA gene recombinant plasmid; M represented DL2000 DNA Marker.
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pone.0119232.g002: Identification of enzyme digestion of 16S rRNA gene recombinant.1, 3 represented blank pGEM-T plasmid; 2, 4 represented enzyme-digested product of 16S rRNA gene recombinant plasmid; M represented DL2000 DNA Marker.

Mentions: PCR amplification results of the 16S rRNA gene from partial patients are shown in Fig. 1. The amplified fragment length was 1532bp. Identification results of restriction enzyme digestion of recombinant plasmid are shown in Fig. 2 and Fig. 3. At least three clones from each PCR ligation reaction per patient were sequenced for the possibility of coexistence of diverse bacteria.


Identification of bacteriology and risk factor analysis of asymptomatic bacterial colonization in pacemaker replacement patients.

Chu XM, Yu H, Sun XX, An Y, Li B, Li XB - PLoS ONE (2015)

Identification of enzyme digestion of 16S rRNA gene recombinant.1, 3 represented blank pGEM-T plasmid; 2, 4 represented enzyme-digested product of 16S rRNA gene recombinant plasmid; M represented DL2000 DNA Marker.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358962&req=5

pone.0119232.g002: Identification of enzyme digestion of 16S rRNA gene recombinant.1, 3 represented blank pGEM-T plasmid; 2, 4 represented enzyme-digested product of 16S rRNA gene recombinant plasmid; M represented DL2000 DNA Marker.
Mentions: PCR amplification results of the 16S rRNA gene from partial patients are shown in Fig. 1. The amplified fragment length was 1532bp. Identification results of restriction enzyme digestion of recombinant plasmid are shown in Fig. 2 and Fig. 3. At least three clones from each PCR ligation reaction per patient were sequenced for the possibility of coexistence of diverse bacteria.

Bottom Line: Twenty two (18.6%) patients had positive bacterial culture results, of which 50% had coagulase-negative staphylococcus.Positive bacterial DNA results from pocket tissues and the surface of the devices were 22.0% and 29.7%, respectively.Bacterial culture combined genetic testing could improve the detection rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, the Affiliated Hospital of Qingdao University, Qingdao, 266100, China.

ABSTRACT

Background: Recent researches revealed that asymptomatic bacterial colonization on PMs might be ubiquitous and increase the risk of clinical PM infection. Early diagnosis of patients with asymptomatic bacterial colonization could provide opportunity for targeted preventive measures.

Objective: The present study explores the incidence of bacterial colonization of generator pockets in pacemaker replacement patients without signs of infection, and to analyze risk factors for asymptomatic bacterial colonization.

Methods: From June 2011 to December 2013, 118 patients underwent pacemaker replacement or upgrade. Identification of bacteria was carried out by bacterial culture and 16S rRNA sequencing. Clinical risk characteristics were analyzed.

Results: The total bacterial positive rate was 37.3% (44 cases), and the coagulase-negative Staphylococcus aureus detection rate was the highest. Twenty two (18.6%) patients had positive bacterial culture results, of which 50% had coagulase-negative staphylococcus. The bacterial DNA detection rate was 36.4 % (43 cases). Positive bacterial DNA results from pocket tissues and the surface of the devices were 22.0% and 29.7%, respectively. During follow-up (median, 27.0 months), three patients (6.8%, 3/44) became symptomatic with the same genus of microorganism, S. aureus (n=2) and S. epidermidis (n=1). Multivariable logistic regression analysis showed that history of bacterial infection, use of antibiotics, application of antiplatelet drugs, replacement frequency were independent risk factors for asymptomatic bacterial colonization.

Conclusion: There was a high incidence of asymptomatic bacterial colonization in pacemaker patients with independent risk factors. Bacterial culture combined genetic testing could improve the detection rate.

Show MeSH
Related in: MedlinePlus