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Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.

Salazar-Peláez LM, Abraham T, Herrera AM, Correa MA, Ortega JE, Paré PD, Seow CY - PLoS ONE (2015)

Bottom Line: The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease.Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group.The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Universidad CES, Medellín, Colombia.

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

No MeSH data available.


Related in: MedlinePlus

Colocalization of MUC5B or MUC5AC and vitronectin.Confocal images showing localization of MUC5B (a) and MUC5AC (b) (green fluorescence), as markers of mucous cells in submucosal glands and epithelial goblet cells, respectively and vitronectin (red fluorescence). Overlay of green and red channels showed that vitronectin is not present in cells producing MUC5B (Rp 0.16, IQR 0.057–0.42) or MUC5AC (Rp 0.04; IQR 0.02–0.13). Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient details of spatial distribution in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. MUC5B and MUC5AC emission intensities are plotted on the x-axis, while vitronectin is plotted on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.
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pone.0119717.g005: Colocalization of MUC5B or MUC5AC and vitronectin.Confocal images showing localization of MUC5B (a) and MUC5AC (b) (green fluorescence), as markers of mucous cells in submucosal glands and epithelial goblet cells, respectively and vitronectin (red fluorescence). Overlay of green and red channels showed that vitronectin is not present in cells producing MUC5B (Rp 0.16, IQR 0.057–0.42) or MUC5AC (Rp 0.04; IQR 0.02–0.13). Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient details of spatial distribution in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. MUC5B and MUC5AC emission intensities are plotted on the x-axis, while vitronectin is plotted on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.

Mentions: For double-labeling assay to detect whether vitronectin is inside mucous cells, there was a median value for the Pearson´s coefficient (0.16, IQR 0.06–0.42) indicating that vitronectin is expressed to a much lower extent in this kind of epithelial gland cell (Fig. 5a). As vitronectin was also observed in the surface epithelium, as illustrated in the images of double-labeling of the subunit alpha-1 of sodium/potassium ATPase and vitronectin, we performed additional staining to evaluate vitronectin’s presence inside the goblet cells (Fig. 5b). Pearson´s colocalization coefficient of MUC5AC and vitronectin (0.04, IQR 0.02–0.13) and visual examination of images with this molecular marker revealed that vitronectin was predominantly present in the apical zone of the surface epithelium, but not inside the goblet cells.


Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.

Salazar-Peláez LM, Abraham T, Herrera AM, Correa MA, Ortega JE, Paré PD, Seow CY - PLoS ONE (2015)

Colocalization of MUC5B or MUC5AC and vitronectin.Confocal images showing localization of MUC5B (a) and MUC5AC (b) (green fluorescence), as markers of mucous cells in submucosal glands and epithelial goblet cells, respectively and vitronectin (red fluorescence). Overlay of green and red channels showed that vitronectin is not present in cells producing MUC5B (Rp 0.16, IQR 0.057–0.42) or MUC5AC (Rp 0.04; IQR 0.02–0.13). Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient details of spatial distribution in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. MUC5B and MUC5AC emission intensities are plotted on the x-axis, while vitronectin is plotted on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358944&req=5

pone.0119717.g005: Colocalization of MUC5B or MUC5AC and vitronectin.Confocal images showing localization of MUC5B (a) and MUC5AC (b) (green fluorescence), as markers of mucous cells in submucosal glands and epithelial goblet cells, respectively and vitronectin (red fluorescence). Overlay of green and red channels showed that vitronectin is not present in cells producing MUC5B (Rp 0.16, IQR 0.057–0.42) or MUC5AC (Rp 0.04; IQR 0.02–0.13). Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient details of spatial distribution in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. MUC5B and MUC5AC emission intensities are plotted on the x-axis, while vitronectin is plotted on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.
Mentions: For double-labeling assay to detect whether vitronectin is inside mucous cells, there was a median value for the Pearson´s coefficient (0.16, IQR 0.06–0.42) indicating that vitronectin is expressed to a much lower extent in this kind of epithelial gland cell (Fig. 5a). As vitronectin was also observed in the surface epithelium, as illustrated in the images of double-labeling of the subunit alpha-1 of sodium/potassium ATPase and vitronectin, we performed additional staining to evaluate vitronectin’s presence inside the goblet cells (Fig. 5b). Pearson´s colocalization coefficient of MUC5AC and vitronectin (0.04, IQR 0.02–0.13) and visual examination of images with this molecular marker revealed that vitronectin was predominantly present in the apical zone of the surface epithelium, but not inside the goblet cells.

Bottom Line: The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease.Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group.The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Universidad CES, Medellín, Colombia.

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

No MeSH data available.


Related in: MedlinePlus