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Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.

Salazar-Peláez LM, Abraham T, Herrera AM, Correa MA, Ortega JE, Paré PD, Seow CY - PLoS ONE (2015)

Bottom Line: The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease.Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group.The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Universidad CES, Medellín, Colombia.

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

No MeSH data available.


Related in: MedlinePlus

Colocalization of vitronectin and lactoferrin or alpha-1 sodium/potassium ATPase (Na+/K+-ATPase).a) Confocal images showing localization of lactoferrin, a marker of serous epithelial cells (green fluorescence), and vitronectin (red fluorescence) in bronchial submucosal glands. Overlay of the green and red channel shows that there is colocalization between lactoferrin and vitronectin, and indicates that vitronectin is inside serous cells in the airway submucosal glands (Rp 0.55, IQR 0.47–0.67). b) Confocal images showing localization of Na+/K+-ATPase (green fluorescence), a marker of cell membrane, and vitronectin (red fluorescence) in a collecting duct of a bronchial gland. Pearson´s correlation coefficient (0.61, IQR 0.54–0.71) shows not only that vitronectin is inside the bronchial epithelial cells, but also is associated with the cell membrane. Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient spatial distribution details in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. Lactoferrin and Na+/K+-ATPase emission intensities are plotted on the x-axis, while vitronectin is on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.
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pone.0119717.g004: Colocalization of vitronectin and lactoferrin or alpha-1 sodium/potassium ATPase (Na+/K+-ATPase).a) Confocal images showing localization of lactoferrin, a marker of serous epithelial cells (green fluorescence), and vitronectin (red fluorescence) in bronchial submucosal glands. Overlay of the green and red channel shows that there is colocalization between lactoferrin and vitronectin, and indicates that vitronectin is inside serous cells in the airway submucosal glands (Rp 0.55, IQR 0.47–0.67). b) Confocal images showing localization of Na+/K+-ATPase (green fluorescence), a marker of cell membrane, and vitronectin (red fluorescence) in a collecting duct of a bronchial gland. Pearson´s correlation coefficient (0.61, IQR 0.54–0.71) shows not only that vitronectin is inside the bronchial epithelial cells, but also is associated with the cell membrane. Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient spatial distribution details in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. Lactoferrin and Na+/K+-ATPase emission intensities are plotted on the x-axis, while vitronectin is on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.

Mentions: Dual labeling experiments with lactoferrin and vitronectin demonstrated that vitronectin is expressed by serous epithelial cells in submucosal glands of the airway tree, as shown in Fig. 4a. In the total sample, the median value of Pearson’s correlation coefficient was 0.55 (IQR 0.47–0.67), indicating a partial interaction between both markers at the subcellular level. Median values for Mander’s colocalization coefficients (MG and MR) for lactoferrin and vitronectin were 0.74 (IQR 0.60–0.79) and 0.93 (IQR 0.89–0.94), respectively. These results suggest that most of the vitronectin molecules are located near lactoferrin molecules. Intracellular presence of vitronectin in serous epithelial cells of bronchial submucosal glands was confirmed by the quantitative colocalization analysis for alpha-1 sodium/potassium ATPase, a marker of cell membrane, and vitronectin (Fig. 4b). Vitronectin was shown to be present inside the serous cells, and more specifically, close to the cell membrane, as shown by median values of Pearson’s correlation coefficient (0.61, IQR 0.54–0.71) and Mander´s colocalization coefficients for the cell membrane marker and vitronectin (MG 0.86, IQR 0.79–0.94; MR 0.84, IQR 0.76–0.90).


Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.

Salazar-Peláez LM, Abraham T, Herrera AM, Correa MA, Ortega JE, Paré PD, Seow CY - PLoS ONE (2015)

Colocalization of vitronectin and lactoferrin or alpha-1 sodium/potassium ATPase (Na+/K+-ATPase).a) Confocal images showing localization of lactoferrin, a marker of serous epithelial cells (green fluorescence), and vitronectin (red fluorescence) in bronchial submucosal glands. Overlay of the green and red channel shows that there is colocalization between lactoferrin and vitronectin, and indicates that vitronectin is inside serous cells in the airway submucosal glands (Rp 0.55, IQR 0.47–0.67). b) Confocal images showing localization of Na+/K+-ATPase (green fluorescence), a marker of cell membrane, and vitronectin (red fluorescence) in a collecting duct of a bronchial gland. Pearson´s correlation coefficient (0.61, IQR 0.54–0.71) shows not only that vitronectin is inside the bronchial epithelial cells, but also is associated with the cell membrane. Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient spatial distribution details in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. Lactoferrin and Na+/K+-ATPase emission intensities are plotted on the x-axis, while vitronectin is on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358944&req=5

pone.0119717.g004: Colocalization of vitronectin and lactoferrin or alpha-1 sodium/potassium ATPase (Na+/K+-ATPase).a) Confocal images showing localization of lactoferrin, a marker of serous epithelial cells (green fluorescence), and vitronectin (red fluorescence) in bronchial submucosal glands. Overlay of the green and red channel shows that there is colocalization between lactoferrin and vitronectin, and indicates that vitronectin is inside serous cells in the airway submucosal glands (Rp 0.55, IQR 0.47–0.67). b) Confocal images showing localization of Na+/K+-ATPase (green fluorescence), a marker of cell membrane, and vitronectin (red fluorescence) in a collecting duct of a bronchial gland. Pearson´s correlation coefficient (0.61, IQR 0.54–0.71) shows not only that vitronectin is inside the bronchial epithelial cells, but also is associated with the cell membrane. Nuclei were counterstained with DAPI (blue fluorescence). xy and xz views are shown to provide sufficient spatial distribution details in the 3D subcellular space. The colocalization maps (scatter plots) of voxels are shown. Lactoferrin and Na+/K+-ATPase emission intensities are plotted on the x-axis, while vitronectin is on the y-axis. Rp, Pearson´s colocalization coefficient; IQR, interquartile range.
Mentions: Dual labeling experiments with lactoferrin and vitronectin demonstrated that vitronectin is expressed by serous epithelial cells in submucosal glands of the airway tree, as shown in Fig. 4a. In the total sample, the median value of Pearson’s correlation coefficient was 0.55 (IQR 0.47–0.67), indicating a partial interaction between both markers at the subcellular level. Median values for Mander’s colocalization coefficients (MG and MR) for lactoferrin and vitronectin were 0.74 (IQR 0.60–0.79) and 0.93 (IQR 0.89–0.94), respectively. These results suggest that most of the vitronectin molecules are located near lactoferrin molecules. Intracellular presence of vitronectin in serous epithelial cells of bronchial submucosal glands was confirmed by the quantitative colocalization analysis for alpha-1 sodium/potassium ATPase, a marker of cell membrane, and vitronectin (Fig. 4b). Vitronectin was shown to be present inside the serous cells, and more specifically, close to the cell membrane, as shown by median values of Pearson’s correlation coefficient (0.61, IQR 0.54–0.71) and Mander´s colocalization coefficients for the cell membrane marker and vitronectin (MG 0.86, IQR 0.79–0.94; MR 0.84, IQR 0.76–0.90).

Bottom Line: The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease.Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group.The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Universidad CES, Medellín, Colombia.

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

No MeSH data available.


Related in: MedlinePlus