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Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.

Salazar-Peláez LM, Abraham T, Herrera AM, Correa MA, Ortega JE, Paré PD, Seow CY - PLoS ONE (2015)

Bottom Line: The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease.Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group.The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Universidad CES, Medellín, Colombia.

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

No MeSH data available.


Related in: MedlinePlus

Lung structural change in asthma and COPD.a) Haematoxylin and eosin staining of bronchial tissues from asthmatic subjects. Arrows point to thickened smooth muscle cell bundles. b) Same staining as in panel a, for tissues from COPD subjects. Arrows point to emphysematous alveolus space.
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pone.0119717.g001: Lung structural change in asthma and COPD.a) Haematoxylin and eosin staining of bronchial tissues from asthmatic subjects. Arrows point to thickened smooth muscle cell bundles. b) Same staining as in panel a, for tissues from COPD subjects. Arrows point to emphysematous alveolus space.

Mentions: Bronchial tissue from a total of 31 subjects: 7 asthmatics, 10 COPD and 14 HC were used for morphological analysis and to assess vitronectin expression. Morphological analysis with H&E of lung tissue from individuals classified as HC showed normal morphology and the lack of inflammatory cells in airway submucosa or signs of tissue remodeling. Bronchial sections from subjects classified as asthma exhibited overall thickening of the airway wall and increased smooth muscle layer (Fig. 1a). COPD individuals exhibited squamous metaplasia, mononuclear cell infiltration, and enlarged alveoli (Fig. 1b).


Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease.

Salazar-Peláez LM, Abraham T, Herrera AM, Correa MA, Ortega JE, Paré PD, Seow CY - PLoS ONE (2015)

Lung structural change in asthma and COPD.a) Haematoxylin and eosin staining of bronchial tissues from asthmatic subjects. Arrows point to thickened smooth muscle cell bundles. b) Same staining as in panel a, for tissues from COPD subjects. Arrows point to emphysematous alveolus space.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358944&req=5

pone.0119717.g001: Lung structural change in asthma and COPD.a) Haematoxylin and eosin staining of bronchial tissues from asthmatic subjects. Arrows point to thickened smooth muscle cell bundles. b) Same staining as in panel a, for tissues from COPD subjects. Arrows point to emphysematous alveolus space.
Mentions: Bronchial tissue from a total of 31 subjects: 7 asthmatics, 10 COPD and 14 HC were used for morphological analysis and to assess vitronectin expression. Morphological analysis with H&E of lung tissue from individuals classified as HC showed normal morphology and the lack of inflammatory cells in airway submucosa or signs of tissue remodeling. Bronchial sections from subjects classified as asthma exhibited overall thickening of the airway wall and increased smooth muscle layer (Fig. 1a). COPD individuals exhibited squamous metaplasia, mononuclear cell infiltration, and enlarged alveoli (Fig. 1b).

Bottom Line: The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease.Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group.The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Universidad CES, Medellín, Colombia.

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.

No MeSH data available.


Related in: MedlinePlus