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Complete blood count reference intervals for healthy Han Chinese adults.

Wu X, Zhao M, Pan B, Zhang J, Peng M, Wang L, Hao X, Huang X, Mu R, Guo W, Qiao R, Chen W, Jiang H, Ma Y, Shang H - PLoS ONE (2015)

Bottom Line: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers.Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference.The reference interval for platelets for the Chengdu center should be established independently.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, China.

ABSTRACT

Background: Complete blood count (CBC) reference intervals are important to diagnose diseases, screen blood donors, and assess overall health. However, current reference intervals established by older instruments and technologies and those from American and European populations are not suitable for Chinese samples due to ethnic, dietary, and lifestyle differences. The aim of this multicenter collaborative study was to establish CBC reference intervals for healthy Han Chinese adults.

Methods: A total of 4,642 healthy individuals (2,136 males and 2,506 females) were recruited from six clinical centers in China (Shenyang, Beijing, Shanghai, Guangzhou, Chengdu, and Xi'an). Blood samples collected in K2EDTA anticoagulant tubes were analyzed. Analysis of variance was performed to determine differences in consensus intervals according to the use of data from the combined sample and selected samples.

Results: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers. Red blood cell count (RBC), hemoglobin (HGB), and hematocrit (HCT) values were higher in males than in females at all ages. Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference. Thalassemia carriers were found to affect the lower or upper limit of different RBC profiles.

Conclusion: We were able to establish consensus intervals for CBC parameters in healthy Han Chinese adults. RBC, HGB, and HCT intervals were established for each sex. The reference interval for platelets for the Chengdu center should be established independently.

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Related in: MedlinePlus

Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) reference intervals for samples with and without thalassemia trait (TT).Percentiles for (A) MCV with TT, (B) MCH with TT, (C) MCV without TT, (D) MCH without TT according to regions.
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pone.0119669.g005: Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) reference intervals for samples with and without thalassemia trait (TT).Percentiles for (A) MCV with TT, (B) MCH with TT, (C) MCV without TT, (D) MCH without TT according to regions.

Mentions: At the Guangzhou and Chengdu centers, TT carriers were identified based on MCV values and thalassemia gene DNA analysis. TT carriers accounted for 10.5% (170/1,622) and 4.3% (39/912) of subjects from these centers, respectively. Inclusion of TT carriers from these centers in the final analysis clearly affected the lower limits of the MCV, HGB, and MCH parameters and the upper limit of the RBC parameter (Table 2, Fig. 5A and 5B), but had little or no impact on the HCT and MCHC intervals. After the exclusion of TT carriers, differences in RBC, HGB, MCV, and MCH percentiles among the six centers were minor, and all data could be combined to determine the consensus intervals for each parameter (Fig. 5C and 5D). TT carriers had an obvious effect on the 2.5th percentiles, but negligible impacts on the median and 97.5th percentiles of the MCV and MCH intervals.


Complete blood count reference intervals for healthy Han Chinese adults.

Wu X, Zhao M, Pan B, Zhang J, Peng M, Wang L, Hao X, Huang X, Mu R, Guo W, Qiao R, Chen W, Jiang H, Ma Y, Shang H - PLoS ONE (2015)

Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) reference intervals for samples with and without thalassemia trait (TT).Percentiles for (A) MCV with TT, (B) MCH with TT, (C) MCV without TT, (D) MCH without TT according to regions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358890&req=5

pone.0119669.g005: Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) reference intervals for samples with and without thalassemia trait (TT).Percentiles for (A) MCV with TT, (B) MCH with TT, (C) MCV without TT, (D) MCH without TT according to regions.
Mentions: At the Guangzhou and Chengdu centers, TT carriers were identified based on MCV values and thalassemia gene DNA analysis. TT carriers accounted for 10.5% (170/1,622) and 4.3% (39/912) of subjects from these centers, respectively. Inclusion of TT carriers from these centers in the final analysis clearly affected the lower limits of the MCV, HGB, and MCH parameters and the upper limit of the RBC parameter (Table 2, Fig. 5A and 5B), but had little or no impact on the HCT and MCHC intervals. After the exclusion of TT carriers, differences in RBC, HGB, MCV, and MCH percentiles among the six centers were minor, and all data could be combined to determine the consensus intervals for each parameter (Fig. 5C and 5D). TT carriers had an obvious effect on the 2.5th percentiles, but negligible impacts on the median and 97.5th percentiles of the MCV and MCH intervals.

Bottom Line: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers.Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference.The reference interval for platelets for the Chengdu center should be established independently.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, China.

ABSTRACT

Background: Complete blood count (CBC) reference intervals are important to diagnose diseases, screen blood donors, and assess overall health. However, current reference intervals established by older instruments and technologies and those from American and European populations are not suitable for Chinese samples due to ethnic, dietary, and lifestyle differences. The aim of this multicenter collaborative study was to establish CBC reference intervals for healthy Han Chinese adults.

Methods: A total of 4,642 healthy individuals (2,136 males and 2,506 females) were recruited from six clinical centers in China (Shenyang, Beijing, Shanghai, Guangzhou, Chengdu, and Xi'an). Blood samples collected in K2EDTA anticoagulant tubes were analyzed. Analysis of variance was performed to determine differences in consensus intervals according to the use of data from the combined sample and selected samples.

Results: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers. Red blood cell count (RBC), hemoglobin (HGB), and hematocrit (HCT) values were higher in males than in females at all ages. Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference. Thalassemia carriers were found to affect the lower or upper limit of different RBC profiles.

Conclusion: We were able to establish consensus intervals for CBC parameters in healthy Han Chinese adults. RBC, HGB, and HCT intervals were established for each sex. The reference interval for platelets for the Chengdu center should be established independently.

Show MeSH
Related in: MedlinePlus