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Complete blood count reference intervals for healthy Han Chinese adults.

Wu X, Zhao M, Pan B, Zhang J, Peng M, Wang L, Hao X, Huang X, Mu R, Guo W, Qiao R, Chen W, Jiang H, Ma Y, Shang H - PLoS ONE (2015)

Bottom Line: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers.Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference.The reference interval for platelets for the Chengdu center should be established independently.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, China.

ABSTRACT

Background: Complete blood count (CBC) reference intervals are important to diagnose diseases, screen blood donors, and assess overall health. However, current reference intervals established by older instruments and technologies and those from American and European populations are not suitable for Chinese samples due to ethnic, dietary, and lifestyle differences. The aim of this multicenter collaborative study was to establish CBC reference intervals for healthy Han Chinese adults.

Methods: A total of 4,642 healthy individuals (2,136 males and 2,506 females) were recruited from six clinical centers in China (Shenyang, Beijing, Shanghai, Guangzhou, Chengdu, and Xi'an). Blood samples collected in K2EDTA anticoagulant tubes were analyzed. Analysis of variance was performed to determine differences in consensus intervals according to the use of data from the combined sample and selected samples.

Results: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers. Red blood cell count (RBC), hemoglobin (HGB), and hematocrit (HCT) values were higher in males than in females at all ages. Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference. Thalassemia carriers were found to affect the lower or upper limit of different RBC profiles.

Conclusion: We were able to establish consensus intervals for CBC parameters in healthy Han Chinese adults. RBC, HGB, and HCT intervals were established for each sex. The reference interval for platelets for the Chengdu center should be established independently.

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Related in: MedlinePlus

Variations in white blood cell (WBC) profile and platelet count (PLT) according to age and sex.(A) WBC, (B) neutrophils (NEUT), (C) lymphocytes (LYM), (D) monocytes (MONO), (E) eosinophils (EO), (F) PLT. *Females show significantly lower values (P < 0.05) than males in the same age group. △Females show significantly higher values (P < 0.05) than males in the same age group. #EO values are significantly higher (P < 0.05) in the 70–79-year age group than in other age groups in males and females, respectively.
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pone.0119669.g004: Variations in white blood cell (WBC) profile and platelet count (PLT) according to age and sex.(A) WBC, (B) neutrophils (NEUT), (C) lymphocytes (LYM), (D) monocytes (MONO), (E) eosinophils (EO), (F) PLT. *Females show significantly lower values (P < 0.05) than males in the same age group. △Females show significantly higher values (P < 0.05) than males in the same age group. #EO values are significantly higher (P < 0.05) in the 70–79-year age group than in other age groups in males and females, respectively.

Mentions: Mean values for the WBC and NEUT parameters were slightly increased in males over 60 years, and were higher in males than in females over the age of 50 years (Fig. 4A and B, P < 0.05). Conversely, LYM values showed a decreasing trend with age in males (Fig. 4C). EO values in males and females tended to increase with age, with an apparent increase in the 70–79-year age group (P < 0.05). EO and MONO counts appeared to be consistently lower in females than in males across all age groups (Fig. 4D and 4E, P < 0.05). In contrast, mean platelet counts were significantly higher in females than in males in all age groups (Fig. 4F, P < 0.05). However, these differences were not sufficiently significant to prompt exclusion from study consensus intervals. Apart from RBC, HGB, and HCT, data for other CBC parameters could be combined to calculate consensus intervals, regardless of age or sex.


Complete blood count reference intervals for healthy Han Chinese adults.

Wu X, Zhao M, Pan B, Zhang J, Peng M, Wang L, Hao X, Huang X, Mu R, Guo W, Qiao R, Chen W, Jiang H, Ma Y, Shang H - PLoS ONE (2015)

Variations in white blood cell (WBC) profile and platelet count (PLT) according to age and sex.(A) WBC, (B) neutrophils (NEUT), (C) lymphocytes (LYM), (D) monocytes (MONO), (E) eosinophils (EO), (F) PLT. *Females show significantly lower values (P < 0.05) than males in the same age group. △Females show significantly higher values (P < 0.05) than males in the same age group. #EO values are significantly higher (P < 0.05) in the 70–79-year age group than in other age groups in males and females, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358890&req=5

pone.0119669.g004: Variations in white blood cell (WBC) profile and platelet count (PLT) according to age and sex.(A) WBC, (B) neutrophils (NEUT), (C) lymphocytes (LYM), (D) monocytes (MONO), (E) eosinophils (EO), (F) PLT. *Females show significantly lower values (P < 0.05) than males in the same age group. △Females show significantly higher values (P < 0.05) than males in the same age group. #EO values are significantly higher (P < 0.05) in the 70–79-year age group than in other age groups in males and females, respectively.
Mentions: Mean values for the WBC and NEUT parameters were slightly increased in males over 60 years, and were higher in males than in females over the age of 50 years (Fig. 4A and B, P < 0.05). Conversely, LYM values showed a decreasing trend with age in males (Fig. 4C). EO values in males and females tended to increase with age, with an apparent increase in the 70–79-year age group (P < 0.05). EO and MONO counts appeared to be consistently lower in females than in males across all age groups (Fig. 4D and 4E, P < 0.05). In contrast, mean platelet counts were significantly higher in females than in males in all age groups (Fig. 4F, P < 0.05). However, these differences were not sufficiently significant to prompt exclusion from study consensus intervals. Apart from RBC, HGB, and HCT, data for other CBC parameters could be combined to calculate consensus intervals, regardless of age or sex.

Bottom Line: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers.Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference.The reference interval for platelets for the Chengdu center should be established independently.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, China.

ABSTRACT

Background: Complete blood count (CBC) reference intervals are important to diagnose diseases, screen blood donors, and assess overall health. However, current reference intervals established by older instruments and technologies and those from American and European populations are not suitable for Chinese samples due to ethnic, dietary, and lifestyle differences. The aim of this multicenter collaborative study was to establish CBC reference intervals for healthy Han Chinese adults.

Methods: A total of 4,642 healthy individuals (2,136 males and 2,506 females) were recruited from six clinical centers in China (Shenyang, Beijing, Shanghai, Guangzhou, Chengdu, and Xi'an). Blood samples collected in K2EDTA anticoagulant tubes were analyzed. Analysis of variance was performed to determine differences in consensus intervals according to the use of data from the combined sample and selected samples.

Results: Median and mean platelet counts from the Chengdu center were significantly lower than those from other centers. Red blood cell count (RBC), hemoglobin (HGB), and hematocrit (HCT) values were higher in males than in females at all ages. Other CBC parameters showed no significant instrument-, region-, age-, or sex-dependent difference. Thalassemia carriers were found to affect the lower or upper limit of different RBC profiles.

Conclusion: We were able to establish consensus intervals for CBC parameters in healthy Han Chinese adults. RBC, HGB, and HCT intervals were established for each sex. The reference interval for platelets for the Chengdu center should be established independently.

Show MeSH
Related in: MedlinePlus