In vitro systems for Atg8 lipidation.
Bottom Line: Among these are the components of two ubiquitin-like conjugation reactions that collectively mediate the conjugation of the ubiquitin-like Atg12 to the Atg5 protein and of the ubiquitin-like protein Atg8 to the headgroup of the membrane lipid phosphatidylethanolamine.The lipidated form of Atg8 is membrane-bound and marks the growing autophagosomal membrane as well as the completed autophagosome.The assays enable the study of the biochemical mechanisms of action of the Atg8 lipidation machinery and to analyze the impact of mutations and post-translational modifications of the conjugation machinery on Atg8 lipidation.
Affiliation: Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9/3, 1030 Vienna, Austria.Show MeSH
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Mentions: A number of genes that are essential for autophagosome formation have been identified, mainly in genetic screen in Saccharomycescerevisiae[4,5]. These genes are conserved from yeast to human and are referred to as autophagy-related genes (ATGs) . A surprising number of these genes functions during the unusual conjugation of the ubiquitin-like Atg8 protein to the headgroup of the membrane lipid phosphatidylethanolamine (PE) on the growing isolation membrane (Fig. 1) [7,8].
Affiliation: Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9/3, 1030 Vienna, Austria.