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Behavior training reverses asymmetry in hippocampal transcriptome of the cav3.2 knockout mice.

Chung NC, Huang YH, Chang CH, Liao JC, Yang CH, Chen CC, Liu IY - PLoS ONE (2015)

Bottom Line: We found a significant left-right asymmetric effect on the hippocampal transcriptome caused by the Cav3.2 knockout.Remarkably, the effect of Cav3.2 knockout was partially reversed by trace fear conditioning.To our knowledge, these results demonstrate for the first time the asymmetric effects of the Cav3.2 and its partial reversal by behavior training on the hippocampal transcriptome.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.

ABSTRACT
Homozygous Cav3.2 knockout mice, which are defective in the pore-forming subunit of a low voltage activated T-type calcium channel, have been documented to show impaired maintenance of late-phase long-term potentiation (L-LTP) and defective retrieval of context-associated fear memory. To investigate the role of Cav3.2 in global gene expression, we performed a microarray transcriptome study on the hippocampi of the Cav3.2-/- mice and their wild-type littermates, either naïve (untrained) or trace fear conditioned. We found a significant left-right asymmetric effect on the hippocampal transcriptome caused by the Cav3.2 knockout. Between the naive Cav3.2-/- and the naive wild-type mice, 3522 differentially expressed genes (DEGs) were found in the left hippocampus, but only 4 DEGs were found in the right hippocampus. Remarkably, the effect of Cav3.2 knockout was partially reversed by trace fear conditioning. The number of DEGs in the left hippocampus was reduced to 6 in the Cav3.2 knockout mice after trace fear conditioning, compared with the wild-type naïve mice. To our knowledge, these results demonstrate for the first time the asymmetric effects of the Cav3.2 and its partial reversal by behavior training on the hippocampal transcriptome.

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The heatmap representing the relative expression level of each DEG over eight sample groups.In the heatmap, color indicates the Z-score of expression relative to the mean over eight conditions. Eight conditions were clustered using hierarchical clustering.
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pone.0118832.g003: The heatmap representing the relative expression level of each DEG over eight sample groups.In the heatmap, color indicates the Z-score of expression relative to the mean over eight conditions. Eight conditions were clustered using hierarchical clustering.

Mentions: Fig. 3 shows the heatmap of the DEG relative expression levels in the eight sample groups. To determine the effects of the Cav3.2 knockout, we compared K and W samples in both N and T groups. In each group, we separated L and R hippocampi samples. To determine the effect of TFC, we compared T and N in both W and K groups, and also separated the samples into L and R hippocampi. To determine the effect of lateralization, we compared L and R in both W and K under T and N conditions (Table 1).


Behavior training reverses asymmetry in hippocampal transcriptome of the cav3.2 knockout mice.

Chung NC, Huang YH, Chang CH, Liao JC, Yang CH, Chen CC, Liu IY - PLoS ONE (2015)

The heatmap representing the relative expression level of each DEG over eight sample groups.In the heatmap, color indicates the Z-score of expression relative to the mean over eight conditions. Eight conditions were clustered using hierarchical clustering.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358833&req=5

pone.0118832.g003: The heatmap representing the relative expression level of each DEG over eight sample groups.In the heatmap, color indicates the Z-score of expression relative to the mean over eight conditions. Eight conditions were clustered using hierarchical clustering.
Mentions: Fig. 3 shows the heatmap of the DEG relative expression levels in the eight sample groups. To determine the effects of the Cav3.2 knockout, we compared K and W samples in both N and T groups. In each group, we separated L and R hippocampi samples. To determine the effect of TFC, we compared T and N in both W and K groups, and also separated the samples into L and R hippocampi. To determine the effect of lateralization, we compared L and R in both W and K under T and N conditions (Table 1).

Bottom Line: We found a significant left-right asymmetric effect on the hippocampal transcriptome caused by the Cav3.2 knockout.Remarkably, the effect of Cav3.2 knockout was partially reversed by trace fear conditioning.To our knowledge, these results demonstrate for the first time the asymmetric effects of the Cav3.2 and its partial reversal by behavior training on the hippocampal transcriptome.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.

ABSTRACT
Homozygous Cav3.2 knockout mice, which are defective in the pore-forming subunit of a low voltage activated T-type calcium channel, have been documented to show impaired maintenance of late-phase long-term potentiation (L-LTP) and defective retrieval of context-associated fear memory. To investigate the role of Cav3.2 in global gene expression, we performed a microarray transcriptome study on the hippocampi of the Cav3.2-/- mice and their wild-type littermates, either naïve (untrained) or trace fear conditioned. We found a significant left-right asymmetric effect on the hippocampal transcriptome caused by the Cav3.2 knockout. Between the naive Cav3.2-/- and the naive wild-type mice, 3522 differentially expressed genes (DEGs) were found in the left hippocampus, but only 4 DEGs were found in the right hippocampus. Remarkably, the effect of Cav3.2 knockout was partially reversed by trace fear conditioning. The number of DEGs in the left hippocampus was reduced to 6 in the Cav3.2 knockout mice after trace fear conditioning, compared with the wild-type naïve mice. To our knowledge, these results demonstrate for the first time the asymmetric effects of the Cav3.2 and its partial reversal by behavior training on the hippocampal transcriptome.

Show MeSH