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Quantitative effect of a CNV on a morphological trait in chickens.

Moro C, Cornette R, Vieaud A, Bruneau N, Gourichon D, Bed'hom B, Tixier-Boichard M - PLoS ONE (2015)

Bottom Line: Analysis of variance showed highly significant effects of line and sex on comb measurements.BW significantly influenced comb mass but not comb size.It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Animal Genetics and Integrative Biology, Jouy-en-Josas, France.

ABSTRACT
Copy Number Variation has been associated with morphological traits, developmental defects or disease susceptibility. The autosomal dominant Pea-comb mutation in chickens is due to the massive amplification of a CNV in intron 1 of SOX5 and provides a unique opportunity to assess the effect of variation in the number of repeats on quantitative traits such as comb size and comb mass in Pea-comb chickens. The quantitative variation of comb size was estimated by 2D morphometry and the number of repeats (RQ) was estimated by qPCR, in a total of 178 chickens from 3 experimental lines, two of them showing segregation for the Pea-comb mutation. This study included only Pea-comb chickens. Analysis of variance showed highly significant effects of line and sex on comb measurements. Adult body weight (BW) and RQ were handled as covariates. BW significantly influenced comb mass but not comb size. RQ values significantly influenced comb size, and the linear regression coefficient was highest for heterozygous carriers: the higher the number of repeats, the smaller the comb size. A similar trend was observed for comb mass. The CNV contributed to 3.4% of the phenotypic variance of comb size in heterozygous carriers of the CNV, an order of magnitude frequently encountered for QTLs. Surprisingly, there was no such relationship between RQ values and comb size in the homozygous line. It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

No MeSH data available.


Related in: MedlinePlus

Variation of comb size as a function of the number of repeats (RQ values) for male heterozygous carriers of the Pea-comb mutation in the WL-DJ and NOE lines.
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pone.0118706.g006: Variation of comb size as a function of the number of repeats (RQ values) for male heterozygous carriers of the Pea-comb mutation in the WL-DJ and NOE lines.

Mentions: The linear regression coefficient of comb size on RQ was negative: it was-13.7 in the analysis of all data, -19.8 in the analysis of the WL-DJ and NOE lines, and-45.57 in the analysis restricted to the heterozygous carriers of Pea-comb. The negative relationship between comb size and RQ values is illustrated on Fig. 6 for male heterozygous carriers of Pea-comb, in the WL-DJ and NOE lines. The proportion of comb size variance explained by the model decreased when the RQ covariate was omitted: the largest loss of variance was observed in the analysis restricted to the heterozygous carriers and represented 3.4% of the total phenotypic variance. In the CH1 line, where all birds are homozygous carriers of Pea-comb, the effect of RQ as a covariate was neither significant for comb size (p = 0.21) nor for comb mass (p = 0.14).


Quantitative effect of a CNV on a morphological trait in chickens.

Moro C, Cornette R, Vieaud A, Bruneau N, Gourichon D, Bed'hom B, Tixier-Boichard M - PLoS ONE (2015)

Variation of comb size as a function of the number of repeats (RQ values) for male heterozygous carriers of the Pea-comb mutation in the WL-DJ and NOE lines.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358827&req=5

pone.0118706.g006: Variation of comb size as a function of the number of repeats (RQ values) for male heterozygous carriers of the Pea-comb mutation in the WL-DJ and NOE lines.
Mentions: The linear regression coefficient of comb size on RQ was negative: it was-13.7 in the analysis of all data, -19.8 in the analysis of the WL-DJ and NOE lines, and-45.57 in the analysis restricted to the heterozygous carriers of Pea-comb. The negative relationship between comb size and RQ values is illustrated on Fig. 6 for male heterozygous carriers of Pea-comb, in the WL-DJ and NOE lines. The proportion of comb size variance explained by the model decreased when the RQ covariate was omitted: the largest loss of variance was observed in the analysis restricted to the heterozygous carriers and represented 3.4% of the total phenotypic variance. In the CH1 line, where all birds are homozygous carriers of Pea-comb, the effect of RQ as a covariate was neither significant for comb size (p = 0.21) nor for comb mass (p = 0.14).

Bottom Line: Analysis of variance showed highly significant effects of line and sex on comb measurements.BW significantly influenced comb mass but not comb size.It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Animal Genetics and Integrative Biology, Jouy-en-Josas, France.

ABSTRACT
Copy Number Variation has been associated with morphological traits, developmental defects or disease susceptibility. The autosomal dominant Pea-comb mutation in chickens is due to the massive amplification of a CNV in intron 1 of SOX5 and provides a unique opportunity to assess the effect of variation in the number of repeats on quantitative traits such as comb size and comb mass in Pea-comb chickens. The quantitative variation of comb size was estimated by 2D morphometry and the number of repeats (RQ) was estimated by qPCR, in a total of 178 chickens from 3 experimental lines, two of them showing segregation for the Pea-comb mutation. This study included only Pea-comb chickens. Analysis of variance showed highly significant effects of line and sex on comb measurements. Adult body weight (BW) and RQ were handled as covariates. BW significantly influenced comb mass but not comb size. RQ values significantly influenced comb size, and the linear regression coefficient was highest for heterozygous carriers: the higher the number of repeats, the smaller the comb size. A similar trend was observed for comb mass. The CNV contributed to 3.4% of the phenotypic variance of comb size in heterozygous carriers of the CNV, an order of magnitude frequently encountered for QTLs. Surprisingly, there was no such relationship between RQ values and comb size in the homozygous line. It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

No MeSH data available.


Related in: MedlinePlus