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Quantitative effect of a CNV on a morphological trait in chickens.

Moro C, Cornette R, Vieaud A, Bruneau N, Gourichon D, Bed'hom B, Tixier-Boichard M - PLoS ONE (2015)

Bottom Line: Analysis of variance showed highly significant effects of line and sex on comb measurements.BW significantly influenced comb mass but not comb size.It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Animal Genetics and Integrative Biology, Jouy-en-Josas, France.

ABSTRACT
Copy Number Variation has been associated with morphological traits, developmental defects or disease susceptibility. The autosomal dominant Pea-comb mutation in chickens is due to the massive amplification of a CNV in intron 1 of SOX5 and provides a unique opportunity to assess the effect of variation in the number of repeats on quantitative traits such as comb size and comb mass in Pea-comb chickens. The quantitative variation of comb size was estimated by 2D morphometry and the number of repeats (RQ) was estimated by qPCR, in a total of 178 chickens from 3 experimental lines, two of them showing segregation for the Pea-comb mutation. This study included only Pea-comb chickens. Analysis of variance showed highly significant effects of line and sex on comb measurements. Adult body weight (BW) and RQ were handled as covariates. BW significantly influenced comb mass but not comb size. RQ values significantly influenced comb size, and the linear regression coefficient was highest for heterozygous carriers: the higher the number of repeats, the smaller the comb size. A similar trend was observed for comb mass. The CNV contributed to 3.4% of the phenotypic variance of comb size in heterozygous carriers of the CNV, an order of magnitude frequently encountered for QTLs. Surprisingly, there was no such relationship between RQ values and comb size in the homozygous line. It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

No MeSH data available.


Related in: MedlinePlus

Frequency distribution of comb size measured in arbitrary units for females of each experimental line (CH1, WL-DJ and NOE).
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pone.0118706.g003: Frequency distribution of comb size measured in arbitrary units for females of each experimental line (CH1, WL-DJ and NOE).

Mentions: In males, comb size varied from 2399 to 9134 units (Fig. 2) and comb mass from 3 to 40.4g (S1 Table). In females, comb size varied from 636 to 4643 (Fig. 3) and comb mass from 0.2 to 5.9g (S1 Table). The coefficients of variation (CV) tended to be higher for comb size in females (24% to 29% according to lines) than in males (15% to 26% according to lines). Comb mass showed much larger CV than comb size, with values ranging from 34% to 54% in males, and from 31 to 55% in females. The CH1 line showed the lowest CV values. In comparison, CV values were much lower for adult body weight, ranging from 8% to 17% in males, and 9% to 20% in females.


Quantitative effect of a CNV on a morphological trait in chickens.

Moro C, Cornette R, Vieaud A, Bruneau N, Gourichon D, Bed'hom B, Tixier-Boichard M - PLoS ONE (2015)

Frequency distribution of comb size measured in arbitrary units for females of each experimental line (CH1, WL-DJ and NOE).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358827&req=5

pone.0118706.g003: Frequency distribution of comb size measured in arbitrary units for females of each experimental line (CH1, WL-DJ and NOE).
Mentions: In males, comb size varied from 2399 to 9134 units (Fig. 2) and comb mass from 3 to 40.4g (S1 Table). In females, comb size varied from 636 to 4643 (Fig. 3) and comb mass from 0.2 to 5.9g (S1 Table). The coefficients of variation (CV) tended to be higher for comb size in females (24% to 29% according to lines) than in males (15% to 26% according to lines). Comb mass showed much larger CV than comb size, with values ranging from 34% to 54% in males, and from 31 to 55% in females. The CH1 line showed the lowest CV values. In comparison, CV values were much lower for adult body weight, ranging from 8% to 17% in males, and 9% to 20% in females.

Bottom Line: Analysis of variance showed highly significant effects of line and sex on comb measurements.BW significantly influenced comb mass but not comb size.It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Animal Genetics and Integrative Biology, Jouy-en-Josas, France.

ABSTRACT
Copy Number Variation has been associated with morphological traits, developmental defects or disease susceptibility. The autosomal dominant Pea-comb mutation in chickens is due to the massive amplification of a CNV in intron 1 of SOX5 and provides a unique opportunity to assess the effect of variation in the number of repeats on quantitative traits such as comb size and comb mass in Pea-comb chickens. The quantitative variation of comb size was estimated by 2D morphometry and the number of repeats (RQ) was estimated by qPCR, in a total of 178 chickens from 3 experimental lines, two of them showing segregation for the Pea-comb mutation. This study included only Pea-comb chickens. Analysis of variance showed highly significant effects of line and sex on comb measurements. Adult body weight (BW) and RQ were handled as covariates. BW significantly influenced comb mass but not comb size. RQ values significantly influenced comb size, and the linear regression coefficient was highest for heterozygous carriers: the higher the number of repeats, the smaller the comb size. A similar trend was observed for comb mass. The CNV contributed to 3.4% of the phenotypic variance of comb size in heterozygous carriers of the CNV, an order of magnitude frequently encountered for QTLs. Surprisingly, there was no such relationship between RQ values and comb size in the homozygous line. It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

No MeSH data available.


Related in: MedlinePlus