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Quantitative effect of a CNV on a morphological trait in chickens.

Moro C, Cornette R, Vieaud A, Bruneau N, Gourichon D, Bed'hom B, Tixier-Boichard M - PLoS ONE (2015)

Bottom Line: Analysis of variance showed highly significant effects of line and sex on comb measurements.BW significantly influenced comb mass but not comb size.It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Animal Genetics and Integrative Biology, Jouy-en-Josas, France.

ABSTRACT
Copy Number Variation has been associated with morphological traits, developmental defects or disease susceptibility. The autosomal dominant Pea-comb mutation in chickens is due to the massive amplification of a CNV in intron 1 of SOX5 and provides a unique opportunity to assess the effect of variation in the number of repeats on quantitative traits such as comb size and comb mass in Pea-comb chickens. The quantitative variation of comb size was estimated by 2D morphometry and the number of repeats (RQ) was estimated by qPCR, in a total of 178 chickens from 3 experimental lines, two of them showing segregation for the Pea-comb mutation. This study included only Pea-comb chickens. Analysis of variance showed highly significant effects of line and sex on comb measurements. Adult body weight (BW) and RQ were handled as covariates. BW significantly influenced comb mass but not comb size. RQ values significantly influenced comb size, and the linear regression coefficient was highest for heterozygous carriers: the higher the number of repeats, the smaller the comb size. A similar trend was observed for comb mass. The CNV contributed to 3.4% of the phenotypic variance of comb size in heterozygous carriers of the CNV, an order of magnitude frequently encountered for QTLs. Surprisingly, there was no such relationship between RQ values and comb size in the homozygous line. It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

No MeSH data available.


Related in: MedlinePlus

Landmark recording on the picture of the Pea-comb from an adult male (animal #2654); G represents the center of gravity.
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pone.0118706.g001: Landmark recording on the picture of the Pea-comb from an adult male (animal #2654); G represents the center of gravity.

Mentions: Image analysis was performed on one picture per bird, corresponding to the one where the comb was the most straight, in order to maximize the surface available for the analysis. Geometric morphometry relies on the Thin Plate Splines approach (TPS) in order to quantify the shape of the comb, and to separate ‘size’ from ‘conformation’ in a second step. The surface of the comb was first outlined on each picture by defining 150 anatomical landmarks between the proximal and the distal ends of the comb (Fig. 1), using the tpsDig2 software.


Quantitative effect of a CNV on a morphological trait in chickens.

Moro C, Cornette R, Vieaud A, Bruneau N, Gourichon D, Bed'hom B, Tixier-Boichard M - PLoS ONE (2015)

Landmark recording on the picture of the Pea-comb from an adult male (animal #2654); G represents the center of gravity.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358827&req=5

pone.0118706.g001: Landmark recording on the picture of the Pea-comb from an adult male (animal #2654); G represents the center of gravity.
Mentions: Image analysis was performed on one picture per bird, corresponding to the one where the comb was the most straight, in order to maximize the surface available for the analysis. Geometric morphometry relies on the Thin Plate Splines approach (TPS) in order to quantify the shape of the comb, and to separate ‘size’ from ‘conformation’ in a second step. The surface of the comb was first outlined on each picture by defining 150 anatomical landmarks between the proximal and the distal ends of the comb (Fig. 1), using the tpsDig2 software.

Bottom Line: Analysis of variance showed highly significant effects of line and sex on comb measurements.BW significantly influenced comb mass but not comb size.It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Animal Genetics and Integrative Biology, Jouy-en-Josas, France.

ABSTRACT
Copy Number Variation has been associated with morphological traits, developmental defects or disease susceptibility. The autosomal dominant Pea-comb mutation in chickens is due to the massive amplification of a CNV in intron 1 of SOX5 and provides a unique opportunity to assess the effect of variation in the number of repeats on quantitative traits such as comb size and comb mass in Pea-comb chickens. The quantitative variation of comb size was estimated by 2D morphometry and the number of repeats (RQ) was estimated by qPCR, in a total of 178 chickens from 3 experimental lines, two of them showing segregation for the Pea-comb mutation. This study included only Pea-comb chickens. Analysis of variance showed highly significant effects of line and sex on comb measurements. Adult body weight (BW) and RQ were handled as covariates. BW significantly influenced comb mass but not comb size. RQ values significantly influenced comb size, and the linear regression coefficient was highest for heterozygous carriers: the higher the number of repeats, the smaller the comb size. A similar trend was observed for comb mass. The CNV contributed to 3.4% of the phenotypic variance of comb size in heterozygous carriers of the CNV, an order of magnitude frequently encountered for QTLs. Surprisingly, there was no such relationship between RQ values and comb size in the homozygous line. It may be concluded that heterozygosity for a CNV in a non-coding region may contribute to phenotypic plasticity.

No MeSH data available.


Related in: MedlinePlus