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Leptin in the canine uterus and placenta: possible implications in pregnancy.

Balogh O, Staub LP, Gram A, Boos A, Kowalewski MP, Reichler IM - Reprod. Biol. Endocrinol. (2015)

Bottom Line: In the Ut/Pl, Lep expression was higher at post-implantation and prepartum luteolysis than at mid-gestation, while in the Ut, Lep mRNA levels did not change during pregnancy.In the Ut, highest LepR mRNA was found at pre- and post-implantation.Aglepristone treatment resulted in a decrease of Lep mRNA levels from 24 to 72 h in the Ut without concomitant changes in the Ut/Pl or in LepR levels.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Leptin (Lep) is known for its involvement in the regulation of reproductive functions. It is important for uterine receptivity, implantation, placental growth and maternal energy homeostasis in several species, but Lep's function in the pregnant dog has not been investigated.

Methods: Pregnant bitches were ovariohysterectomized at pre-implantation, post-implantation, mid-gestation and prepartum luteolysis. Two additional groups were treated with aglepristone in mid-gestation, and ovariohysterectomized 24 or 72 h later. Lep and leptin receptor (LepR) gene expression was detected by semi-quantitative real-time PCR in pre-implantation and inter-placental uterine sections (Ut) and in utero-placental compartments (Ut/Pl). Immunohistochemistry and in situ hybridization (ISH) were performed for Lep and LepR protein and mRNA localization. Parametric one-way ANOVA, paired t-test and Wilcoxon signed-rank test were used for statistical analysis.

Results: In the Ut/Pl, Lep expression was higher at post-implantation and prepartum luteolysis than at mid-gestation, while in the Ut, Lep mRNA levels did not change during pregnancy. LepR expression in the Ut/Pl was up-regulated at prepartum luteolysis compared to the earlier stages. In the Ut, highest LepR mRNA was found at pre- and post-implantation. LepR expression was down-regulated in the Ut/Pl compared to the Ut at post-implantation and at mid-gestation. Aglepristone treatment resulted in a decrease of Lep mRNA levels from 24 to 72 h in the Ut without concomitant changes in the Ut/Pl or in LepR levels. Lep and LepR immunoreactivities were strong in the luminal and glandular epithelium in the Ut with abundant LepR signals in the subepithelial stroma. In the Ut/Pl, fetal trophoblasts stained stronger for Lep and LepR than decidual cells, and signals for both proteins were also detected in the glandular chambers. The myometrium, blood vessel media, and sporadically also the endothelium stained for Lep and LepR. ISH showed similar signal distribution in the Ut and Ut/Pl.

Conclusions: Lep and LepR are differentially expressed in the canine uterus and placenta during pregnancy, and their presence in various cell types indicates paracrine/autocrine roles. The Lep signaling system may be one of the pathways involved in feto-maternal cross-talk, implantation and maintenance of pregnancy, and may have a regulatory role around parturition.

No MeSH data available.


Related in: MedlinePlus

Leptin gene expression in the utero-placental and uterine compartments during pregnancy. Leptin gene expression during pregnancy in the utero-placental compartments (A), and in the pre-implantation uterus and inter-placental uterine sections (B). RGE: relative gene expression. Bars and whiskers present the geometric mean and the deviation factor. Bars with different superscripts differ at P ≤ 0.043.
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Fig1: Leptin gene expression in the utero-placental and uterine compartments during pregnancy. Leptin gene expression during pregnancy in the utero-placental compartments (A), and in the pre-implantation uterus and inter-placental uterine sections (B). RGE: relative gene expression. Bars and whiskers present the geometric mean and the deviation factor. Bars with different superscripts differ at P ≤ 0.043.

Mentions: In the Ut/Pl, we found lower Lep mRNA concentrations at mid-gestation than at post-implantation and prepartum luteolysis (P ≤ 0.043; Figure 1A). In the Ut, Lep expression did not change throughout gestation (P = 0.12; Figure 1B). Lep mRNA levels were similar between the Ut/Pl and Ut both at the post-implantation (P = 0.87) and mid-gestation stages (P = 1.00; Figure 2A).Figure 1


Leptin in the canine uterus and placenta: possible implications in pregnancy.

Balogh O, Staub LP, Gram A, Boos A, Kowalewski MP, Reichler IM - Reprod. Biol. Endocrinol. (2015)

Leptin gene expression in the utero-placental and uterine compartments during pregnancy. Leptin gene expression during pregnancy in the utero-placental compartments (A), and in the pre-implantation uterus and inter-placental uterine sections (B). RGE: relative gene expression. Bars and whiskers present the geometric mean and the deviation factor. Bars with different superscripts differ at P ≤ 0.043.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4358730&req=5

Fig1: Leptin gene expression in the utero-placental and uterine compartments during pregnancy. Leptin gene expression during pregnancy in the utero-placental compartments (A), and in the pre-implantation uterus and inter-placental uterine sections (B). RGE: relative gene expression. Bars and whiskers present the geometric mean and the deviation factor. Bars with different superscripts differ at P ≤ 0.043.
Mentions: In the Ut/Pl, we found lower Lep mRNA concentrations at mid-gestation than at post-implantation and prepartum luteolysis (P ≤ 0.043; Figure 1A). In the Ut, Lep expression did not change throughout gestation (P = 0.12; Figure 1B). Lep mRNA levels were similar between the Ut/Pl and Ut both at the post-implantation (P = 0.87) and mid-gestation stages (P = 1.00; Figure 2A).Figure 1

Bottom Line: In the Ut/Pl, Lep expression was higher at post-implantation and prepartum luteolysis than at mid-gestation, while in the Ut, Lep mRNA levels did not change during pregnancy.In the Ut, highest LepR mRNA was found at pre- and post-implantation.Aglepristone treatment resulted in a decrease of Lep mRNA levels from 24 to 72 h in the Ut without concomitant changes in the Ut/Pl or in LepR levels.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Leptin (Lep) is known for its involvement in the regulation of reproductive functions. It is important for uterine receptivity, implantation, placental growth and maternal energy homeostasis in several species, but Lep's function in the pregnant dog has not been investigated.

Methods: Pregnant bitches were ovariohysterectomized at pre-implantation, post-implantation, mid-gestation and prepartum luteolysis. Two additional groups were treated with aglepristone in mid-gestation, and ovariohysterectomized 24 or 72 h later. Lep and leptin receptor (LepR) gene expression was detected by semi-quantitative real-time PCR in pre-implantation and inter-placental uterine sections (Ut) and in utero-placental compartments (Ut/Pl). Immunohistochemistry and in situ hybridization (ISH) were performed for Lep and LepR protein and mRNA localization. Parametric one-way ANOVA, paired t-test and Wilcoxon signed-rank test were used for statistical analysis.

Results: In the Ut/Pl, Lep expression was higher at post-implantation and prepartum luteolysis than at mid-gestation, while in the Ut, Lep mRNA levels did not change during pregnancy. LepR expression in the Ut/Pl was up-regulated at prepartum luteolysis compared to the earlier stages. In the Ut, highest LepR mRNA was found at pre- and post-implantation. LepR expression was down-regulated in the Ut/Pl compared to the Ut at post-implantation and at mid-gestation. Aglepristone treatment resulted in a decrease of Lep mRNA levels from 24 to 72 h in the Ut without concomitant changes in the Ut/Pl or in LepR levels. Lep and LepR immunoreactivities were strong in the luminal and glandular epithelium in the Ut with abundant LepR signals in the subepithelial stroma. In the Ut/Pl, fetal trophoblasts stained stronger for Lep and LepR than decidual cells, and signals for both proteins were also detected in the glandular chambers. The myometrium, blood vessel media, and sporadically also the endothelium stained for Lep and LepR. ISH showed similar signal distribution in the Ut and Ut/Pl.

Conclusions: Lep and LepR are differentially expressed in the canine uterus and placenta during pregnancy, and their presence in various cell types indicates paracrine/autocrine roles. The Lep signaling system may be one of the pathways involved in feto-maternal cross-talk, implantation and maintenance of pregnancy, and may have a regulatory role around parturition.

No MeSH data available.


Related in: MedlinePlus