Limits...
Artemisinin mimics calorie restriction to trigger mitochondrial biogenesis and compromise telomere shortening in mice.

Wang DT, He J, Wu M, Li SM, Gao Q, Zeng QP - PeerJ (2015)

Bottom Line: Calorie restriction is known to extend lifespan among organisms by a debating mechanism underlying nitric oxide-driven mitochondrial biogenesis.We report here that nitric oxide generators including artemisinin, sodium nitroprusside, and L-arginine mimics calorie restriction and resembles hydrogen peroxide to initiate the nitric oxide signaling cascades and elicit the global antioxidative responses in mice.In conclusion, calorie restriction-triggered nitric oxide provides antioxidative protection and alleviates telomere attrition via mitochondrial biogenesis, thereby maintaining chromosomal stability and integrity, which are the hallmarks of longevity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Tropical Medicine Institute, Guangzhou University of Chinese Medicine , Guangzhou , China.

ABSTRACT
Calorie restriction is known to extend lifespan among organisms by a debating mechanism underlying nitric oxide-driven mitochondrial biogenesis. We report here that nitric oxide generators including artemisinin, sodium nitroprusside, and L-arginine mimics calorie restriction and resembles hydrogen peroxide to initiate the nitric oxide signaling cascades and elicit the global antioxidative responses in mice. The large quantities of antioxidant enzymes are correlated with the low levels of reactive oxygen species, which allow the down-regulation of tumor suppressors and accessory DNA repair partners, eventually leading to the compromise of telomere shortening. Accompanying with the up-regulation of signal transducers and respiratory chain signatures, mitochondrial biogenesis occurs with the elevation of adenosine triphosphate levels upon exposure of mouse skeletal muscles to the mimetics of calorie restriction. In conclusion, calorie restriction-triggered nitric oxide provides antioxidative protection and alleviates telomere attrition via mitochondrial biogenesis, thereby maintaining chromosomal stability and integrity, which are the hallmarks of longevity.

No MeSH data available.


Related in: MedlinePlus

Hybridization detection of TRFs in mouse skeletal muscle cells of AL, CR and mimetics-treated mice.For Southern blotting, samples were collected from the skeletal muscle of AL and CR mice, or from CR mimetics mice injected by 260 µM ART, 67 µM SNP, 5.7 mM ARG, or 200 µM H2O2 in the dose of 50 µl volume/20 g body weight for three times, in which the 1st, 2nd, and 3rd injections are on the 1st, 3rd, and 5th day, respectively. The ages of AL and CR mimetics-treated mice are two-month-old, and the ages of CR mice are five-month-old, including one-month AL and four-month CR treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4358698&req=5

fig-4: Hybridization detection of TRFs in mouse skeletal muscle cells of AL, CR and mimetics-treated mice.For Southern blotting, samples were collected from the skeletal muscle of AL and CR mice, or from CR mimetics mice injected by 260 µM ART, 67 µM SNP, 5.7 mM ARG, or 200 µM H2O2 in the dose of 50 µl volume/20 g body weight for three times, in which the 1st, 2nd, and 3rd injections are on the 1st, 3rd, and 5th day, respectively. The ages of AL and CR mimetics-treated mice are two-month-old, and the ages of CR mice are five-month-old, including one-month AL and four-month CR treatment.

Mentions: From the results regarding the global down-regulation of tumor suppressor genes and accessory DNA repair genes by CR and mimetics, it can be expected that mice treated by CR and mimetics should have longer telomeres. To confirm this deduction, we compared the lengths of telomere restriction fragments (TRFs) from the skeletal muscle cells of CR and mimetics-treated mice to those of an AL mouse. Consequently, TRFs of an AL sample were found to shift faster than those of ART, SNP, ARG, H2O2, and CR samples on the gel, suggesting AL TRFs being shorter than ART, SNP, ARG, H2O2, and CR TRFs (Fig. 4).


Artemisinin mimics calorie restriction to trigger mitochondrial biogenesis and compromise telomere shortening in mice.

Wang DT, He J, Wu M, Li SM, Gao Q, Zeng QP - PeerJ (2015)

Hybridization detection of TRFs in mouse skeletal muscle cells of AL, CR and mimetics-treated mice.For Southern blotting, samples were collected from the skeletal muscle of AL and CR mice, or from CR mimetics mice injected by 260 µM ART, 67 µM SNP, 5.7 mM ARG, or 200 µM H2O2 in the dose of 50 µl volume/20 g body weight for three times, in which the 1st, 2nd, and 3rd injections are on the 1st, 3rd, and 5th day, respectively. The ages of AL and CR mimetics-treated mice are two-month-old, and the ages of CR mice are five-month-old, including one-month AL and four-month CR treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358698&req=5

fig-4: Hybridization detection of TRFs in mouse skeletal muscle cells of AL, CR and mimetics-treated mice.For Southern blotting, samples were collected from the skeletal muscle of AL and CR mice, or from CR mimetics mice injected by 260 µM ART, 67 µM SNP, 5.7 mM ARG, or 200 µM H2O2 in the dose of 50 µl volume/20 g body weight for three times, in which the 1st, 2nd, and 3rd injections are on the 1st, 3rd, and 5th day, respectively. The ages of AL and CR mimetics-treated mice are two-month-old, and the ages of CR mice are five-month-old, including one-month AL and four-month CR treatment.
Mentions: From the results regarding the global down-regulation of tumor suppressor genes and accessory DNA repair genes by CR and mimetics, it can be expected that mice treated by CR and mimetics should have longer telomeres. To confirm this deduction, we compared the lengths of telomere restriction fragments (TRFs) from the skeletal muscle cells of CR and mimetics-treated mice to those of an AL mouse. Consequently, TRFs of an AL sample were found to shift faster than those of ART, SNP, ARG, H2O2, and CR samples on the gel, suggesting AL TRFs being shorter than ART, SNP, ARG, H2O2, and CR TRFs (Fig. 4).

Bottom Line: Calorie restriction is known to extend lifespan among organisms by a debating mechanism underlying nitric oxide-driven mitochondrial biogenesis.We report here that nitric oxide generators including artemisinin, sodium nitroprusside, and L-arginine mimics calorie restriction and resembles hydrogen peroxide to initiate the nitric oxide signaling cascades and elicit the global antioxidative responses in mice.In conclusion, calorie restriction-triggered nitric oxide provides antioxidative protection and alleviates telomere attrition via mitochondrial biogenesis, thereby maintaining chromosomal stability and integrity, which are the hallmarks of longevity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Tropical Medicine Institute, Guangzhou University of Chinese Medicine , Guangzhou , China.

ABSTRACT
Calorie restriction is known to extend lifespan among organisms by a debating mechanism underlying nitric oxide-driven mitochondrial biogenesis. We report here that nitric oxide generators including artemisinin, sodium nitroprusside, and L-arginine mimics calorie restriction and resembles hydrogen peroxide to initiate the nitric oxide signaling cascades and elicit the global antioxidative responses in mice. The large quantities of antioxidant enzymes are correlated with the low levels of reactive oxygen species, which allow the down-regulation of tumor suppressors and accessory DNA repair partners, eventually leading to the compromise of telomere shortening. Accompanying with the up-regulation of signal transducers and respiratory chain signatures, mitochondrial biogenesis occurs with the elevation of adenosine triphosphate levels upon exposure of mouse skeletal muscles to the mimetics of calorie restriction. In conclusion, calorie restriction-triggered nitric oxide provides antioxidative protection and alleviates telomere attrition via mitochondrial biogenesis, thereby maintaining chromosomal stability and integrity, which are the hallmarks of longevity.

No MeSH data available.


Related in: MedlinePlus