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In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice.

Abu N, Mohamed NE, Yeap SK, Lim KL, Akhtar MN, Zulfadli AJ, Kee BB, Abdullah MP, Omar AR, Alitheen NB - Drug Des Devel Ther (2015)

Bottom Line: Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro.However, the in vivo antitumor effects of FKB have not been reported on yet.All in all, FKB may serve as a promising anticancer agent, especially in treating breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Bright Sparks Unit, Universiti Malaya, Kuala Lumpur, Malaysia ; Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.

ABSTRACT
Flavokawain B (FKB) is a naturally occurring chalcone that can be isolated through the root extracts of the kava-kava plant (Piper methysticum). It can also be synthesized chemically to increase the yield. This compound is a promising candidate as a biological agent, as it is reported to be involved in a wide range of biological activities. Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro. However, the in vivo antitumor effects of FKB have not been reported on yet. Breast cancer is one of the major causes of cancer-related deaths in the world today. Any potential treatment should not only impede the growth of the tumor, but also modulate the immune system efficiently and inhibit the formation of secondary tumors. As presented in our study, FKB induced apoptosis in 4T1 tumors in vivo, as evidenced by the terminal deoxynucleotidyl transferase dUTP nick end labeling and hematoxylin and eosin staining of the tumor. FKB also regulated the immune system by increasing both helper and cytolytic T-cell and natural killer cell populations. In addition, FKB also enhanced the levels of interleukin 2 and interferon gamma but suppressed interleukin 1B. Apart from that, FKB was also found to inhibit metastasis, as evaluated by clonogenic assay, bone marrow smearing assay, real-time polymerase chain reaction, Western blot, and proteome profiler analysis. All in all, FKB may serve as a promising anticancer agent, especially in treating breast cancer.

No MeSH data available.


Related in: MedlinePlus

The direct and indirect effects of flavokawain B in regulating the immune system, metastasis, and inflammatory processes in relation to cancer progression.
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f14-dddt-9-1401: The direct and indirect effects of flavokawain B in regulating the immune system, metastasis, and inflammatory processes in relation to cancer progression.

Mentions: On the basis of the results obtained from this study, FKB managed to halt the proliferation of 4T1 cells and the migration and invasiveness in vitro. This chalcone also reduced the size of the tumor and induced apoptosis in vivo. Moreover, FKB impeded the metastatic process in vivo as well as reduced the inflammatory process. Figure 14 depicts the possible direct and indirect mechanism of the activity of flavokawain in vivo, based on the results of this study. Nevertheless, more in-depth study should be conducted to fully understand the mechanism of FKB. Overall, FKB has a bright prospect of becoming an anticancer agent, and it should be further developed into the pharmaceutical line, especially for treating breast cancer.


In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice.

Abu N, Mohamed NE, Yeap SK, Lim KL, Akhtar MN, Zulfadli AJ, Kee BB, Abdullah MP, Omar AR, Alitheen NB - Drug Des Devel Ther (2015)

The direct and indirect effects of flavokawain B in regulating the immune system, metastasis, and inflammatory processes in relation to cancer progression.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358690&req=5

f14-dddt-9-1401: The direct and indirect effects of flavokawain B in regulating the immune system, metastasis, and inflammatory processes in relation to cancer progression.
Mentions: On the basis of the results obtained from this study, FKB managed to halt the proliferation of 4T1 cells and the migration and invasiveness in vitro. This chalcone also reduced the size of the tumor and induced apoptosis in vivo. Moreover, FKB impeded the metastatic process in vivo as well as reduced the inflammatory process. Figure 14 depicts the possible direct and indirect mechanism of the activity of flavokawain in vivo, based on the results of this study. Nevertheless, more in-depth study should be conducted to fully understand the mechanism of FKB. Overall, FKB has a bright prospect of becoming an anticancer agent, and it should be further developed into the pharmaceutical line, especially for treating breast cancer.

Bottom Line: Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro.However, the in vivo antitumor effects of FKB have not been reported on yet.All in all, FKB may serve as a promising anticancer agent, especially in treating breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Bright Sparks Unit, Universiti Malaya, Kuala Lumpur, Malaysia ; Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.

ABSTRACT
Flavokawain B (FKB) is a naturally occurring chalcone that can be isolated through the root extracts of the kava-kava plant (Piper methysticum). It can also be synthesized chemically to increase the yield. This compound is a promising candidate as a biological agent, as it is reported to be involved in a wide range of biological activities. Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro. However, the in vivo antitumor effects of FKB have not been reported on yet. Breast cancer is one of the major causes of cancer-related deaths in the world today. Any potential treatment should not only impede the growth of the tumor, but also modulate the immune system efficiently and inhibit the formation of secondary tumors. As presented in our study, FKB induced apoptosis in 4T1 tumors in vivo, as evidenced by the terminal deoxynucleotidyl transferase dUTP nick end labeling and hematoxylin and eosin staining of the tumor. FKB also regulated the immune system by increasing both helper and cytolytic T-cell and natural killer cell populations. In addition, FKB also enhanced the levels of interleukin 2 and interferon gamma but suppressed interleukin 1B. Apart from that, FKB was also found to inhibit metastasis, as evaluated by clonogenic assay, bone marrow smearing assay, real-time polymerase chain reaction, Western blot, and proteome profiler analysis. All in all, FKB may serve as a promising anticancer agent, especially in treating breast cancer.

No MeSH data available.


Related in: MedlinePlus