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Development modeling of Lucilia sericata (Diptera: Calliphoridae).

Roe A, Higley LG - PeerJ (2015)

Bottom Line: Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures.Temperature-induced mortality was highest from 10.0 to 17.5 °C and 32.5 °C.The development data generated illustrates the advantages of large datasets in modeling Lucilia sericata development and the need for curvilinear models in describing development at environmental temperatures near the biological minima and maxima.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biology Program, College of Saint Mary , Omaha, NE , United States.

ABSTRACT
The relationship between insect development and temperature has been well established and has a wide range of uses, including the use of blow flies for postmortem (PMI) interval estimations in death investigations. To use insects in estimating PMI, we must be able to determine the insect age at the time of discovery and backtrack to time of oviposition. Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures. A series of experiments were conducted with Lucilia sericata, a forensically important blow fly species, that met the requirements needed to create statistically valid development models. Experiments were conducted over 11 temperatures (7.5 to 32.5 °C, at 2.5 °C) with a 16:8 L:D cycle. Experimental units contained 20 eggs, 10 g beef liver, and 2.5 cm of pine shavings. Each life stage (egg to adult) had five sampling times. Each sampling time was replicated four times, for a total of 20 measurements per life stage. For each sampling time, the cups were pulled from the chambers and the stage of each maggot was documented morphologically through posterior spiracle slits and cephalopharyngeal skeletal development. Data were normally distributed with the later larval stages (L3f, L3m) having the most variation within and transitioning between stages. The biological minimum was between 7.5 °C and 10 °C, with little egg development and no egg emergence at 7.5 °C. Temperature-induced mortality was highest from 10.0 to 17.5 °C and 32.5 °C. The development data generated illustrates the advantages of large datasets in modeling Lucilia sericata development and the need for curvilinear models in describing development at environmental temperatures near the biological minima and maxima.

No MeSH data available.


Related in: MedlinePlus

Development rates of Lucilia sericata by stage.From 10.0 to 32.5 °C, with 95% confidence intervals represented by dotted lines, Life stages egg-L2 are indicated in 1B and stages L3-P in 1C.
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fig-1: Development rates of Lucilia sericata by stage.From 10.0 to 32.5 °C, with 95% confidence intervals represented by dotted lines, Life stages egg-L2 are indicated in 1B and stages L3-P in 1C.

Mentions: We observed substantial variation in stage transition times (Roe, 2014) and stage durations comparable to that reported by Tarone & Foran (2006). The largest variation was observed during the L3m and pupation stages, regardless of temperature, with the variation largest at 10.0 °C through 17.5 °C (L3m and pupation) and 32.5 °C (L3m) (Fig. 1). These stages are also the longest life stages (by proportion) (Table 2).


Development modeling of Lucilia sericata (Diptera: Calliphoridae).

Roe A, Higley LG - PeerJ (2015)

Development rates of Lucilia sericata by stage.From 10.0 to 32.5 °C, with 95% confidence intervals represented by dotted lines, Life stages egg-L2 are indicated in 1B and stages L3-P in 1C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358662&req=5

fig-1: Development rates of Lucilia sericata by stage.From 10.0 to 32.5 °C, with 95% confidence intervals represented by dotted lines, Life stages egg-L2 are indicated in 1B and stages L3-P in 1C.
Mentions: We observed substantial variation in stage transition times (Roe, 2014) and stage durations comparable to that reported by Tarone & Foran (2006). The largest variation was observed during the L3m and pupation stages, regardless of temperature, with the variation largest at 10.0 °C through 17.5 °C (L3m and pupation) and 32.5 °C (L3m) (Fig. 1). These stages are also the longest life stages (by proportion) (Table 2).

Bottom Line: Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures.Temperature-induced mortality was highest from 10.0 to 17.5 °C and 32.5 °C.The development data generated illustrates the advantages of large datasets in modeling Lucilia sericata development and the need for curvilinear models in describing development at environmental temperatures near the biological minima and maxima.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biology Program, College of Saint Mary , Omaha, NE , United States.

ABSTRACT
The relationship between insect development and temperature has been well established and has a wide range of uses, including the use of blow flies for postmortem (PMI) interval estimations in death investigations. To use insects in estimating PMI, we must be able to determine the insect age at the time of discovery and backtrack to time of oviposition. Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures. A series of experiments were conducted with Lucilia sericata, a forensically important blow fly species, that met the requirements needed to create statistically valid development models. Experiments were conducted over 11 temperatures (7.5 to 32.5 °C, at 2.5 °C) with a 16:8 L:D cycle. Experimental units contained 20 eggs, 10 g beef liver, and 2.5 cm of pine shavings. Each life stage (egg to adult) had five sampling times. Each sampling time was replicated four times, for a total of 20 measurements per life stage. For each sampling time, the cups were pulled from the chambers and the stage of each maggot was documented morphologically through posterior spiracle slits and cephalopharyngeal skeletal development. Data were normally distributed with the later larval stages (L3f, L3m) having the most variation within and transitioning between stages. The biological minimum was between 7.5 °C and 10 °C, with little egg development and no egg emergence at 7.5 °C. Temperature-induced mortality was highest from 10.0 to 17.5 °C and 32.5 °C. The development data generated illustrates the advantages of large datasets in modeling Lucilia sericata development and the need for curvilinear models in describing development at environmental temperatures near the biological minima and maxima.

No MeSH data available.


Related in: MedlinePlus