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Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification.

Zeng WJ, Peng CW, Yuan JP, Cui R, Li Y - Int J Nanomedicine (2015)

Bottom Line: This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins.Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People's Republic of China.

ABSTRACT

Purpose: The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification.

Methods: A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features.

Results: The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes (P<0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.

Conclusion: The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

No MeSH data available.


Related in: MedlinePlus

Four different interactions of cancer cells and immunocytes in MA.Notes: The QD-based multiplexed imaging technique stained cancer cells green, macrophages yellow, and lymphocytes red. Cancer cells (green arrow) were surrounded by lymphocytes (red arrow) (A1 and A2). Macrophages (yellow arrow) approached cancer cells (green arrow) but could not surround them (B1 and B2). Macrophages (yellow arrow) were surrounded by cancer cells (green arrow) (C1 and C2). Cancer cells (green arrow) and immunocytes (red arrow) indicate lymphocytes in (D1), and yellow arrow indicates macrophages in (D2) were unrelated. MA cells spears (A1–D1). MA cells paraffin embedded sections (A2–D2). Scale bar: 50 μm for all images (A1–D2). Magnifications: 200× for all images (A1–D2).Abbreviations: MA, malignant ascites; QD, quantum dot.
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f3-ijn-10-1759: Four different interactions of cancer cells and immunocytes in MA.Notes: The QD-based multiplexed imaging technique stained cancer cells green, macrophages yellow, and lymphocytes red. Cancer cells (green arrow) were surrounded by lymphocytes (red arrow) (A1 and A2). Macrophages (yellow arrow) approached cancer cells (green arrow) but could not surround them (B1 and B2). Macrophages (yellow arrow) were surrounded by cancer cells (green arrow) (C1 and C2). Cancer cells (green arrow) and immunocytes (red arrow) indicate lymphocytes in (D1), and yellow arrow indicates macrophages in (D2) were unrelated. MA cells spears (A1–D1). MA cells paraffin embedded sections (A2–D2). Scale bar: 50 μm for all images (A1–D2). Magnifications: 200× for all images (A1–D2).Abbreviations: MA, malignant ascites; QD, quantum dot.

Mentions: By using this QD-based multiplexed imaging technology of GC tissue as a basis, we have established an optimized protocol for QD-based multiplexed imaging of cancer cells and immunocytes in MA. This technology revealed four types of interactions between cancer cells and immunocytes in the MA (Figure 3): 1) a few cancer cells were surrounded by numerous lymphocytes (Figures 3A1 and 3A2); this finding suggests enhanced host immune reactions against cancer cells in MA; 2) cancer cells were approached but could not be surrounded by macrophages (Figures 3B1 and 3B2); this finding suggests the reduced ability of the host's immune system to limit cancer cells; 3) numerous cancer cells surrounded the macrophages (Figures 3C1 and 3C2); this finding indicates significantly compromised host immune reactions against cancer cells; and 4) cancer cells and immunocytes were unrelated (Figures 3D1 and 3D2); this finding suggests possible host immune tolerance for cancer cells.


Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification.

Zeng WJ, Peng CW, Yuan JP, Cui R, Li Y - Int J Nanomedicine (2015)

Four different interactions of cancer cells and immunocytes in MA.Notes: The QD-based multiplexed imaging technique stained cancer cells green, macrophages yellow, and lymphocytes red. Cancer cells (green arrow) were surrounded by lymphocytes (red arrow) (A1 and A2). Macrophages (yellow arrow) approached cancer cells (green arrow) but could not surround them (B1 and B2). Macrophages (yellow arrow) were surrounded by cancer cells (green arrow) (C1 and C2). Cancer cells (green arrow) and immunocytes (red arrow) indicate lymphocytes in (D1), and yellow arrow indicates macrophages in (D2) were unrelated. MA cells spears (A1–D1). MA cells paraffin embedded sections (A2–D2). Scale bar: 50 μm for all images (A1–D2). Magnifications: 200× for all images (A1–D2).Abbreviations: MA, malignant ascites; QD, quantum dot.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4356691&req=5

f3-ijn-10-1759: Four different interactions of cancer cells and immunocytes in MA.Notes: The QD-based multiplexed imaging technique stained cancer cells green, macrophages yellow, and lymphocytes red. Cancer cells (green arrow) were surrounded by lymphocytes (red arrow) (A1 and A2). Macrophages (yellow arrow) approached cancer cells (green arrow) but could not surround them (B1 and B2). Macrophages (yellow arrow) were surrounded by cancer cells (green arrow) (C1 and C2). Cancer cells (green arrow) and immunocytes (red arrow) indicate lymphocytes in (D1), and yellow arrow indicates macrophages in (D2) were unrelated. MA cells spears (A1–D1). MA cells paraffin embedded sections (A2–D2). Scale bar: 50 μm for all images (A1–D2). Magnifications: 200× for all images (A1–D2).Abbreviations: MA, malignant ascites; QD, quantum dot.
Mentions: By using this QD-based multiplexed imaging technology of GC tissue as a basis, we have established an optimized protocol for QD-based multiplexed imaging of cancer cells and immunocytes in MA. This technology revealed four types of interactions between cancer cells and immunocytes in the MA (Figure 3): 1) a few cancer cells were surrounded by numerous lymphocytes (Figures 3A1 and 3A2); this finding suggests enhanced host immune reactions against cancer cells in MA; 2) cancer cells were approached but could not be surrounded by macrophages (Figures 3B1 and 3B2); this finding suggests the reduced ability of the host's immune system to limit cancer cells; 3) numerous cancer cells surrounded the macrophages (Figures 3C1 and 3C2); this finding indicates significantly compromised host immune reactions against cancer cells; and 4) cancer cells and immunocytes were unrelated (Figures 3D1 and 3D2); this finding suggests possible host immune tolerance for cancer cells.

Bottom Line: This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins.Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People's Republic of China.

ABSTRACT

Purpose: The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification.

Methods: A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features.

Results: The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes (P<0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.

Conclusion: The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

No MeSH data available.


Related in: MedlinePlus