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Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification.

Zeng WJ, Peng CW, Yuan JP, Cui R, Li Y - Int J Nanomedicine (2015)

Bottom Line: This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins.Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People's Republic of China.

ABSTRACT

Purpose: The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification.

Methods: A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features.

Results: The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes (P<0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.

Conclusion: The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

No MeSH data available.


Related in: MedlinePlus

Multicolor QD-based IHC staining for GC tissue.Notes: GC tissue marked with cancer cell are green (green arrow), with macrophages are yellow (yellow arrow), and with lymphocytes are red (red arrow). Scale bar: 20 μm. Magnification for all images: 400×.Abbreviations: QD, quantum dot; IHC, immunohistochemistry; GC, gastric cancer.
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f2-ijn-10-1759: Multicolor QD-based IHC staining for GC tissue.Notes: GC tissue marked with cancer cell are green (green arrow), with macrophages are yellow (yellow arrow), and with lymphocytes are red (red arrow). Scale bar: 20 μm. Magnification for all images: 400×.Abbreviations: QD, quantum dot; IHC, immunohistochemistry; GC, gastric cancer.

Mentions: Then, a QD-based multiplexed imaging technique was developed to simultaneously reveal the presence of GC cells, macrophages, and lymphocytes in the tumor microenvironment (Figure 2). It was found that macrophages and lymphocytes tended to present lineal distribution synchronously.


Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification.

Zeng WJ, Peng CW, Yuan JP, Cui R, Li Y - Int J Nanomedicine (2015)

Multicolor QD-based IHC staining for GC tissue.Notes: GC tissue marked with cancer cell are green (green arrow), with macrophages are yellow (yellow arrow), and with lymphocytes are red (red arrow). Scale bar: 20 μm. Magnification for all images: 400×.Abbreviations: QD, quantum dot; IHC, immunohistochemistry; GC, gastric cancer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356691&req=5

f2-ijn-10-1759: Multicolor QD-based IHC staining for GC tissue.Notes: GC tissue marked with cancer cell are green (green arrow), with macrophages are yellow (yellow arrow), and with lymphocytes are red (red arrow). Scale bar: 20 μm. Magnification for all images: 400×.Abbreviations: QD, quantum dot; IHC, immunohistochemistry; GC, gastric cancer.
Mentions: Then, a QD-based multiplexed imaging technique was developed to simultaneously reveal the presence of GC cells, macrophages, and lymphocytes in the tumor microenvironment (Figure 2). It was found that macrophages and lymphocytes tended to present lineal distribution synchronously.

Bottom Line: This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins.Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People's Republic of China.

ABSTRACT

Purpose: The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification.

Methods: A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features.

Results: The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes (P<0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.

Conclusion: The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

No MeSH data available.


Related in: MedlinePlus