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Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.


Related in: MedlinePlus

TEM images of the samples: (A) MCS-15, (B) magnified local area in (A), (C) MCS-4, and (D) high-resolution TEM image of MCS-4.Note: Arrows indicate the mesoporous structure and pore size.Abbreviations: MCS, mesoporous calcium–silicon; TEM, transmission electron microscope.
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f6-ijn-10-1715: TEM images of the samples: (A) MCS-15, (B) magnified local area in (A), (C) MCS-4, and (D) high-resolution TEM image of MCS-4.Note: Arrows indicate the mesoporous structure and pore size.Abbreviations: MCS, mesoporous calcium–silicon; TEM, transmission electron microscope.

Mentions: The morphology and microstructure of the MCS-15 examined by TEM are shown in Figure 6. The MCS-15 synthesized using TMB showed a disordered mesoporous structure, as well as pretty thick pore walls and larger pore size (15 nm), whereas MCS-4 synthesized without using TMB possessed a highly ordered pore structure with a smaller pore size and thicker pore walls. The SEM images of the surface morphology of the MCS-15 are shown in Figure 7. The MCS-15 was made of irregular particles with an interconnected porous structure, while the MCS-4 was made of regular short rodlike particles without obvious pores on the surface of the particles.


Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

TEM images of the samples: (A) MCS-15, (B) magnified local area in (A), (C) MCS-4, and (D) high-resolution TEM image of MCS-4.Note: Arrows indicate the mesoporous structure and pore size.Abbreviations: MCS, mesoporous calcium–silicon; TEM, transmission electron microscope.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356665&req=5

f6-ijn-10-1715: TEM images of the samples: (A) MCS-15, (B) magnified local area in (A), (C) MCS-4, and (D) high-resolution TEM image of MCS-4.Note: Arrows indicate the mesoporous structure and pore size.Abbreviations: MCS, mesoporous calcium–silicon; TEM, transmission electron microscope.
Mentions: The morphology and microstructure of the MCS-15 examined by TEM are shown in Figure 6. The MCS-15 synthesized using TMB showed a disordered mesoporous structure, as well as pretty thick pore walls and larger pore size (15 nm), whereas MCS-4 synthesized without using TMB possessed a highly ordered pore structure with a smaller pore size and thicker pore walls. The SEM images of the surface morphology of the MCS-15 are shown in Figure 7. The MCS-15 was made of irregular particles with an interconnected porous structure, while the MCS-4 was made of regular short rodlike particles without obvious pores on the surface of the particles.

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.


Related in: MedlinePlus