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Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.


Pore diameter distribution of the samples MCS-15 (A) and MCS-4 (B).Abbreviation: MCS, mesoporous calcium–silicon.
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f5-ijn-10-1715: Pore diameter distribution of the samples MCS-15 (A) and MCS-4 (B).Abbreviation: MCS, mesoporous calcium–silicon.

Mentions: The N2 adsorption–desorption isotherms and pore diameter distribution of the MC1-15 and MCS-4 are shown in Figure 4A and B and Figure 5A and B, respectively, which are very different, indicating the distinctive pore structures. In the isotherm of MCS-15 that was synthesized using a pore-expanding agent, a sudden jump in the amount of adsorbed N2 within a P/P0 range from 0.8 to 1.0 was found. The BET specific surface area, pore size, and pore volume of MCS-15 were 257.8 m2/g, 15 nm, and 1.43 cm3/g, respectively. However, the isotherm of MCS-4 was a typical type IV isotherm, in which the hysteresis loop was clearly evident, implying a highly consistent pore structure. The BET surface area, pore size, and pore volume of MCS-4 were 485.6 m2/g, 4 nm, and 0.48 cm3/g, respectively, considerably different from those of MCS-15.


Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

Pore diameter distribution of the samples MCS-15 (A) and MCS-4 (B).Abbreviation: MCS, mesoporous calcium–silicon.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356665&req=5

f5-ijn-10-1715: Pore diameter distribution of the samples MCS-15 (A) and MCS-4 (B).Abbreviation: MCS, mesoporous calcium–silicon.
Mentions: The N2 adsorption–desorption isotherms and pore diameter distribution of the MC1-15 and MCS-4 are shown in Figure 4A and B and Figure 5A and B, respectively, which are very different, indicating the distinctive pore structures. In the isotherm of MCS-15 that was synthesized using a pore-expanding agent, a sudden jump in the amount of adsorbed N2 within a P/P0 range from 0.8 to 1.0 was found. The BET specific surface area, pore size, and pore volume of MCS-15 were 257.8 m2/g, 15 nm, and 1.43 cm3/g, respectively. However, the isotherm of MCS-4 was a typical type IV isotherm, in which the hysteresis loop was clearly evident, implying a highly consistent pore structure. The BET surface area, pore size, and pore volume of MCS-4 were 485.6 m2/g, 4 nm, and 0.48 cm3/g, respectively, considerably different from those of MCS-15.

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.