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Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.


ALP activity of hMSCs cultured on MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 4 and 7 days (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: ALP, alkaline phosphatase; DNA, deoxyribonucleic acid; hMSCs, human mesenchymal stem cells; MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein.
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f12-ijn-10-1715: ALP activity of hMSCs cultured on MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 4 and 7 days (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: ALP, alkaline phosphatase; DNA, deoxyribonucleic acid; hMSCs, human mesenchymal stem cells; MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein.

Mentions: ALP activity of hMSCs cultured on MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems was assessed at 4 and 7 days. Figure 12 reveals that the ALP activities for both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems were significantly higher than those for MCS-15 and MCS-4 without rhBMP-2 at 4 and 7 days (P<0.05). At 4 days, ALP expressed at lower levels, and no significant differences were detected for both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems. However, the ALP activity of hMSCs on MCS-15/rhBMP-2 system was significantly greater than that on MCS-4/rhBMP-2 at 7 days (P<0.05).


Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

ALP activity of hMSCs cultured on MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 4 and 7 days (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: ALP, alkaline phosphatase; DNA, deoxyribonucleic acid; hMSCs, human mesenchymal stem cells; MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356665&req=5

f12-ijn-10-1715: ALP activity of hMSCs cultured on MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 4 and 7 days (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: ALP, alkaline phosphatase; DNA, deoxyribonucleic acid; hMSCs, human mesenchymal stem cells; MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein.
Mentions: ALP activity of hMSCs cultured on MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems was assessed at 4 and 7 days. Figure 12 reveals that the ALP activities for both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems were significantly higher than those for MCS-15 and MCS-4 without rhBMP-2 at 4 and 7 days (P<0.05). At 4 days, ALP expressed at lower levels, and no significant differences were detected for both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems. However, the ALP activity of hMSCs on MCS-15/rhBMP-2 system was significantly greater than that on MCS-4/rhBMP-2 at 7 days (P<0.05).

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.