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Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.


Proliferation of hMSCs cultured on both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 1, 3, and 5 days through the mitochondrial activity measurement (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein; hMSCs, human mesenchymal stem cells.
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f10-ijn-10-1715: Proliferation of hMSCs cultured on both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 1, 3, and 5 days through the mitochondrial activity measurement (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein; hMSCs, human mesenchymal stem cells.

Mentions: Proliferation of hMSCs cultured on both the MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems was assessed through the mitochondrial activity measurement, using the MTT assay. Figure 10 reveals that the OD values for all the samples increased with time, and the OD values for both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems were significantly higher than those for MCS-15 and MCS-4 without rhBMP-2 at 1, 3, and 5 days (P<0.05). In addition, the OD value for MCS-4/rhBMP-2 was higher than that for MCS-15/rhBMP-2 system at 1 and 3 days because of more rhBMP-2 release from MCS-4/rhBMP-2 (initial burst release from MCS-4). However, the OD value for MCS-15/rhBMP-2 system was significantly higher than that for MCS-4/rhBMP-2 at 5 days (P<0.05). The results indicated that MCS-15/rhBMP-2 system with sustained release of rhBMP-2 facilitated continued cell proliferation.


Mesoporous calcium-silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2.

Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J - Int J Nanomedicine (2015)

Proliferation of hMSCs cultured on both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 1, 3, and 5 days through the mitochondrial activity measurement (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein; hMSCs, human mesenchymal stem cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356665&req=5

f10-ijn-10-1715: Proliferation of hMSCs cultured on both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 at 1, 3, and 5 days through the mitochondrial activity measurement (MCS-15 and MCS-4 without loaded rhBMP-2 as controls).Note: *P<0.05.Abbreviations: MCS, mesoporous calcium–silicon; rhBMP, recombinant human bone morphogenetic protein; hMSCs, human mesenchymal stem cells.
Mentions: Proliferation of hMSCs cultured on both the MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems was assessed through the mitochondrial activity measurement, using the MTT assay. Figure 10 reveals that the OD values for all the samples increased with time, and the OD values for both MCS-15/rhBMP-2 and MCS-4/rhBMP-2 systems were significantly higher than those for MCS-15 and MCS-4 without rhBMP-2 at 1, 3, and 5 days (P<0.05). In addition, the OD value for MCS-4/rhBMP-2 was higher than that for MCS-15/rhBMP-2 system at 1 and 3 days because of more rhBMP-2 release from MCS-4/rhBMP-2 (initial burst release from MCS-4). However, the OD value for MCS-15/rhBMP-2 system was significantly higher than that for MCS-4/rhBMP-2 at 5 days (P<0.05). The results indicated that MCS-15/rhBMP-2 system with sustained release of rhBMP-2 facilitated continued cell proliferation.

Bottom Line: The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2).Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release).The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.

ABSTRACT
Mesoporous calcium-silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm(3)/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium-silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

No MeSH data available.