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Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey.

Öztürk Y, Çevik B - Plant Pathol. J. (2015)

Bottom Line: Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world.Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups.While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Food Agriculture and Livestock, Eğirdir Fruit Research Station, 32500, Isparta, Turkey.

ABSTRACT
Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

No MeSH data available.


Related in: MedlinePlus

Symptoms observed in sweet cherry trees with suspected PDV infection. (A) Chlorotic ring spots on leaves. (B) Deformations and wrinkles along the veins on leaves. (C) Leaf elongation. (D) Density of teeth near the leaf edge.
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f1-ppj-31-41: Symptoms observed in sweet cherry trees with suspected PDV infection. (A) Chlorotic ring spots on leaves. (B) Deformations and wrinkles along the veins on leaves. (C) Leaf elongation. (D) Density of teeth near the leaf edge.

Mentions: Leaf samples were collected from 521 trees from 142 commercial orchards in 10 different districts of Isparta Province. The samples were distributed to the districts proportionally considering to amount of sweet cherry production area and amount production. Sweet cherry trees from which the samples were collected were generally dwarf compared to the surrounding trees and showed general chlorosis and had ruptured, narrowed and thinned leaves and thin and leafy lateral shoots. Symptoms of shot holes, chlorotic ring spots and mosaic were also observed in leaves of these trees. Some of the sampled trees showed only one of these symptoms while others displayed several symptoms together. ‘0900 Ziraat’ was the major sweet cherry variety grown in surveyed orchards, however, samples were also collected from ‘Starks Gold’ (white cherry) and ‘Sweet Heart’ varieties. Fig. 1 shows symptoms observed in some of the collected samples suspected to be infected with PDV. These samples and some symptomless leaves were later tested PDV positive in the laboratory.


Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey.

Öztürk Y, Çevik B - Plant Pathol. J. (2015)

Symptoms observed in sweet cherry trees with suspected PDV infection. (A) Chlorotic ring spots on leaves. (B) Deformations and wrinkles along the veins on leaves. (C) Leaf elongation. (D) Density of teeth near the leaf edge.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4356604&req=5

f1-ppj-31-41: Symptoms observed in sweet cherry trees with suspected PDV infection. (A) Chlorotic ring spots on leaves. (B) Deformations and wrinkles along the veins on leaves. (C) Leaf elongation. (D) Density of teeth near the leaf edge.
Mentions: Leaf samples were collected from 521 trees from 142 commercial orchards in 10 different districts of Isparta Province. The samples were distributed to the districts proportionally considering to amount of sweet cherry production area and amount production. Sweet cherry trees from which the samples were collected were generally dwarf compared to the surrounding trees and showed general chlorosis and had ruptured, narrowed and thinned leaves and thin and leafy lateral shoots. Symptoms of shot holes, chlorotic ring spots and mosaic were also observed in leaves of these trees. Some of the sampled trees showed only one of these symptoms while others displayed several symptoms together. ‘0900 Ziraat’ was the major sweet cherry variety grown in surveyed orchards, however, samples were also collected from ‘Starks Gold’ (white cherry) and ‘Sweet Heart’ varieties. Fig. 1 shows symptoms observed in some of the collected samples suspected to be infected with PDV. These samples and some symptomless leaves were later tested PDV positive in the laboratory.

Bottom Line: Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world.Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups.While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Food Agriculture and Livestock, Eğirdir Fruit Research Station, 32500, Isparta, Turkey.

ABSTRACT
Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

No MeSH data available.


Related in: MedlinePlus