Limits...
Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus

Development of F2 segregating population and analysis of marker linked to SV1 gene. “+”, Presence of amplicon; “−”, Absence of amplicon. 1Genotypes (+)-SV1SV1SV2SV2 (1), SV1SV1SV2sv2 (2), SV1SV1sv2sv2 (1), SV1sv1SV2SV2 (2), SV1sv1SV2sv2 (4), SV1sv1sv2sv2 (2); 2Genotypes (−)-sv1sv1sv2sv2 (1); 3Genotypes (+)-0; 4Genotypes (−)-sv1sv1SV2SV2 (1), sv1sv1SV2sv2 (2). Figures in the parentheses are the table χ2 values at 5% level of significance.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4356603&req=5

f4-ppj-31-33: Development of F2 segregating population and analysis of marker linked to SV1 gene. “+”, Presence of amplicon; “−”, Absence of amplicon. 1Genotypes (+)-SV1SV1SV2SV2 (1), SV1SV1SV2sv2 (2), SV1SV1sv2sv2 (1), SV1sv1SV2SV2 (2), SV1sv1SV2sv2 (4), SV1sv1sv2sv2 (2); 2Genotypes (−)-sv1sv1sv2sv2 (1); 3Genotypes (+)-0; 4Genotypes (−)-sv1sv1SV2SV2 (1), sv1sv1SV2sv2 (2). Figures in the parentheses are the table χ2 values at 5% level of significance.

Mentions: Furthermore, the IABTPPN7983 marker strongly associated with PSMD responsive SV1 (inhibitory) gene for PPSMV Bidar isolate (Fig. 4). However, another marker IABTPPN7414 did not record any linkage/association with SV2 (resistant) or SV1 gene. Linkage analysis showed that the marker IABTPPN7983 was located 23.9 cM away from the SV1 gene. In order to determine the usefulness of the RAPD marker, a set of 4 resistant, 1 moderately resistance and 7 susceptible genotypes were screened with primer IABTPPN7. Marker IABTPPN7983 was recoded in 5 out of 7 susceptible genotypes (71.4%) and absent in 1 of the 4 resistant genotypes (25%). Similarly, marker IABTPPN7414 was absent in 2 of the 7 susceptible genotypes (28.5%) and present in 3 out of 4 resistant genotypes (75%). Additionally, marker IABTPPN7983 and IABTPPN7414 was absent in moderately resistance genotype.


Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Development of F2 segregating population and analysis of marker linked to SV1 gene. “+”, Presence of amplicon; “−”, Absence of amplicon. 1Genotypes (+)-SV1SV1SV2SV2 (1), SV1SV1SV2sv2 (2), SV1SV1sv2sv2 (1), SV1sv1SV2SV2 (2), SV1sv1SV2sv2 (4), SV1sv1sv2sv2 (2); 2Genotypes (−)-sv1sv1sv2sv2 (1); 3Genotypes (+)-0; 4Genotypes (−)-sv1sv1SV2SV2 (1), sv1sv1SV2sv2 (2). Figures in the parentheses are the table χ2 values at 5% level of significance.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4356603&req=5

f4-ppj-31-33: Development of F2 segregating population and analysis of marker linked to SV1 gene. “+”, Presence of amplicon; “−”, Absence of amplicon. 1Genotypes (+)-SV1SV1SV2SV2 (1), SV1SV1SV2sv2 (2), SV1SV1sv2sv2 (1), SV1sv1SV2SV2 (2), SV1sv1SV2sv2 (4), SV1sv1sv2sv2 (2); 2Genotypes (−)-sv1sv1sv2sv2 (1); 3Genotypes (+)-0; 4Genotypes (−)-sv1sv1SV2SV2 (1), sv1sv1SV2sv2 (2). Figures in the parentheses are the table χ2 values at 5% level of significance.
Mentions: Furthermore, the IABTPPN7983 marker strongly associated with PSMD responsive SV1 (inhibitory) gene for PPSMV Bidar isolate (Fig. 4). However, another marker IABTPPN7414 did not record any linkage/association with SV2 (resistant) or SV1 gene. Linkage analysis showed that the marker IABTPPN7983 was located 23.9 cM away from the SV1 gene. In order to determine the usefulness of the RAPD marker, a set of 4 resistant, 1 moderately resistance and 7 susceptible genotypes were screened with primer IABTPPN7. Marker IABTPPN7983 was recoded in 5 out of 7 susceptible genotypes (71.4%) and absent in 1 of the 4 resistant genotypes (25%). Similarly, marker IABTPPN7414 was absent in 2 of the 7 susceptible genotypes (28.5%) and present in 3 out of 4 resistant genotypes (75%). Additionally, marker IABTPPN7983 and IABTPPN7414 was absent in moderately resistance genotype.

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus