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Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus

Segregation of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 across F2 plant DNA of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.
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f3-ppj-31-33: Segregation of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 across F2 plant DNA of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.

Mentions: Primer IABTPPN7 was used to amplify DNA from all 325 F2 plants to confirm linkage with PSMD resistance and determine the rate of recombination (Fig. 3). Among the 325 F2 plants, IABTPPN7983 marker was absent in 68 and present in 257 F2 individual plants. Segregation of both PSMD susceptible and IABTPPN7983 marker in the F2 showed that IABTPPN7983 was present in 1 resistant and absent in 54 resistant plants, present in 256 susceptible plants, absent in 14 susceptible plants (Supplementary Table 1). Similarly, IABTPPN7414 marker was recorded in 255 F2 and absent in 70 F2 individual plants. The resistance bulk and parent specific amplicon was present in 53 out of 55 resistance F2 plant and absent in 68 out of 270 F2 susceptible plants (Supplementary Table 2). The segregation pattern for both the short decamer random DNA marker amplicons, IABTPPN7414 and IABTPPN7983 showed goodness of fit to 3:1 ratio, typical segregation ratio of a dominant marker (Table 1). Above these two IABTPPN7983 and IABTPPN7414 markers results which differed significantly from the expected 9:3:3:1 ratio (Table 2).


Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Segregation of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 across F2 plant DNA of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4356603&req=5

f3-ppj-31-33: Segregation of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 across F2 plant DNA of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.
Mentions: Primer IABTPPN7 was used to amplify DNA from all 325 F2 plants to confirm linkage with PSMD resistance and determine the rate of recombination (Fig. 3). Among the 325 F2 plants, IABTPPN7983 marker was absent in 68 and present in 257 F2 individual plants. Segregation of both PSMD susceptible and IABTPPN7983 marker in the F2 showed that IABTPPN7983 was present in 1 resistant and absent in 54 resistant plants, present in 256 susceptible plants, absent in 14 susceptible plants (Supplementary Table 1). Similarly, IABTPPN7414 marker was recorded in 255 F2 and absent in 70 F2 individual plants. The resistance bulk and parent specific amplicon was present in 53 out of 55 resistance F2 plant and absent in 68 out of 270 F2 susceptible plants (Supplementary Table 2). The segregation pattern for both the short decamer random DNA marker amplicons, IABTPPN7414 and IABTPPN7983 showed goodness of fit to 3:1 ratio, typical segregation ratio of a dominant marker (Table 1). Above these two IABTPPN7983 and IABTPPN7414 markers results which differed significantly from the expected 9:3:3:1 ratio (Table 2).

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus