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Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus

Screening of the seven resistant and seven susceptible F2 plant DNA of the cross Gullyal white × BSMR 736 with a coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414, linked to PSMD. SP=PSMD susceptible parent Gullyal white, RP=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.
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f2-ppj-31-33: Screening of the seven resistant and seven susceptible F2 plant DNA of the cross Gullyal white × BSMR 736 with a coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414, linked to PSMD. SP=PSMD susceptible parent Gullyal white, RP=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.

Mentions: Coupling phase associated IABTPPN7414 and repulsion phase associated IABTPPN7983 markers were further tested against the subset of resistance and susceptible DNA bulks. The IABTPPN7414 decamer random DNA marker amplicon amplified the polymorphic fragment only in the resistance parent and resistance F2 segregants and it was clearly absent in susceptible parent and susceptible F2 segregants. However, IABTPPN7983 decamer random DNA marker amplicon amplified the polymorphic fragment only in the susceptible parent and susceptible F2 segregants and it was clearly absent in resistance parent and resistance F2 segregants (Fig. 2).


Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Screening of the seven resistant and seven susceptible F2 plant DNA of the cross Gullyal white × BSMR 736 with a coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414, linked to PSMD. SP=PSMD susceptible parent Gullyal white, RP=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4356603&req=5

f2-ppj-31-33: Screening of the seven resistant and seven susceptible F2 plant DNA of the cross Gullyal white × BSMR 736 with a coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414, linked to PSMD. SP=PSMD susceptible parent Gullyal white, RP=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.
Mentions: Coupling phase associated IABTPPN7414 and repulsion phase associated IABTPPN7983 markers were further tested against the subset of resistance and susceptible DNA bulks. The IABTPPN7414 decamer random DNA marker amplicon amplified the polymorphic fragment only in the resistance parent and resistance F2 segregants and it was clearly absent in susceptible parent and susceptible F2 segregants. However, IABTPPN7983 decamer random DNA marker amplicon amplified the polymorphic fragment only in the susceptible parent and susceptible F2 segregants and it was clearly absent in resistance parent and resistance F2 segregants (Fig. 2).

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus