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Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus

Amplification pattern of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 in parents and resistant and susceptible bulks. M, 100 bp ladder DNA; RP, Resistant parent - BSMR 736; RB, resistant bulk; SP, susceptible parent - Gullyal white; SB, susceptible bulk.
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f1-ppj-31-33: Amplification pattern of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 in parents and resistant and susceptible bulks. M, 100 bp ladder DNA; RP, Resistant parent - BSMR 736; RB, resistant bulk; SP, susceptible parent - Gullyal white; SB, susceptible bulk.

Mentions: A set of 300 short decamer random DNA primers screened to detect polymorphism between resistance parent (BSMR 736) and susceptible parent (Gullyal white). Out of these 300 decamer random DNA primers, 32 primers (10.6%) repeatedly detected the polymorphic loci in parents. Thirteen primers in susceptible parent, 10 primers in resistant parent were found to be polymorphic; a set of 9 primers viz IABTPPAB12, IABTPPAB16, IABTPPH1, IABTPPH4, IABTPPH6, IABTPPN10, IABTPPN7, IABTPPN14 and IABTPPAB16) showed codominant nature polymorphism. Out of 9 co-dominant decamer random DNA primers, IABTPPN 7 recorded distinct, repeatable and high degree of polymorphism in the resistant parent, resistant bulk, susceptible parent and susceptible bulk. IABTPPN7 generated unique polymorphic DNA amplicom of approximately 1000 bp and 450 bp, after sequencing they were confirmed as 983 bp (IABTPPN7983) and 414 bp (IABTPPN7414), respectively. Out of these two markers identified, IABTPPN7414 was associated in coupling phase to the resistant allele, while IABTPPN7983 was linked in repulsion phase to the susceptible allele (Fig. 1). However, except IABTPPN7 other 8 primers could not distinguish parent and bulk.


Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.).

Daspute A, Fakrudin B - Plant Pathol. J. (2015)

Amplification pattern of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 in parents and resistant and susceptible bulks. M, 100 bp ladder DNA; RP, Resistant parent - BSMR 736; RB, resistant bulk; SP, susceptible parent - Gullyal white; SB, susceptible bulk.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4356603&req=5

f1-ppj-31-33: Amplification pattern of coupling phase RAPD markers IABTPPN7983 and repulsion phase RAPD marker IABTPPN7414 in parents and resistant and susceptible bulks. M, 100 bp ladder DNA; RP, Resistant parent - BSMR 736; RB, resistant bulk; SP, susceptible parent - Gullyal white; SB, susceptible bulk.
Mentions: A set of 300 short decamer random DNA primers screened to detect polymorphism between resistance parent (BSMR 736) and susceptible parent (Gullyal white). Out of these 300 decamer random DNA primers, 32 primers (10.6%) repeatedly detected the polymorphic loci in parents. Thirteen primers in susceptible parent, 10 primers in resistant parent were found to be polymorphic; a set of 9 primers viz IABTPPAB12, IABTPPAB16, IABTPPH1, IABTPPH4, IABTPPH6, IABTPPN10, IABTPPN7, IABTPPN14 and IABTPPAB16) showed codominant nature polymorphism. Out of 9 co-dominant decamer random DNA primers, IABTPPN 7 recorded distinct, repeatable and high degree of polymorphism in the resistant parent, resistant bulk, susceptible parent and susceptible bulk. IABTPPN7 generated unique polymorphic DNA amplicom of approximately 1000 bp and 450 bp, after sequencing they were confirmed as 983 bp (IABTPPN7983) and 414 bp (IABTPPN7414), respectively. Out of these two markers identified, IABTPPN7414 was associated in coupling phase to the resistant allele, while IABTPPN7983 was linked in repulsion phase to the susceptible allele (Fig. 1). However, except IABTPPN7 other 8 primers could not distinguish parent and bulk.

Bottom Line: On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance.Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population.The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India.

ABSTRACT
Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F2 population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

No MeSH data available.


Related in: MedlinePlus