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Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs.

Paolini A, Curti V, Pasi F, Mazzini G, Nano R, Capelli E - Int. J. Oncol. (2015)

Bottom Line: The anticancer effect of this drug has been based on its antioxidant effects.The results confirmed in the T98G cells the anti-proliferative effect of GA reported for other glioma cell lines and showed that the miRNA expression changes depending on GA concentrations.Thus, the key for GA to induce a specific anticancer action is to use an optimal concentration that avoids these twin effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biotechnology, Laboratory of Neuro Radio Experimental Biology, 'Lazzaro Spallanzani', University of Pavia, 27100 Pavia, Italy.

ABSTRACT
Glioblastoma multiforme (GBM) is the most malignant primary brain tumor in adulthood, characterized by very high recurrence. Following the limited results for conventional therapies, novel therapeutic agents are under investigation. Among the putative new molecules, gallic acid (GA) represents a promising new anticancer drug. The anticancer effect of this drug has been based on its antioxidant effects. The aim of the present study was to investigate the toxic effects of GA on the T98G human glioblastoma cell line and its capacity to modulate the expression of microRNAs targeting the genes involved in tumor growth and invasion. Cytotoxicity, clonogenic ability and cell migration after GA treatment were tested. Moreover, the expression of miRNAs that target genes for antioxidant mitochondrial enzymes (mir-17-3p), p-21 protein (mir-21-5p) and ATM (mir-421-5p) was determined by qRT-PCR. The results confirmed in the T98G cells the anti-proliferative effect of GA reported for other glioma cell lines and showed that the miRNA expression changes depending on GA concentrations. Different GA concentrations can determine a protective or a toxic effect on tumor cells. Thus, the key for GA to induce a specific anticancer action is to use an optimal concentration that avoids these twin effects.

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MicroRNA expression of miR-21-5p, miR-17-3p, miR-421-5p in T98G cells after treatment with gallic acid at different concentration. qRT-PCR.
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f5-ijo-46-04-1491: MicroRNA expression of miR-21-5p, miR-17-3p, miR-421-5p in T98G cells after treatment with gallic acid at different concentration. qRT-PCR.

Mentions: The expression of these miRNAs was determined by qRT-PCR (see Materials and methods) and the results are reported in Fig. 5. The miRNAs considered showed a variation in their expression after GA treatment displaying a common reduced expression at low GA concentrations (1.2, 12.5 and 25 μg/ml) and an increased expression at concentrations >25 μg/ml (Fig. 5). The expression of the three different miRNAs seems to be modulated by different GA concentrations with a significant increase at the concentration of 75 μg/ml and a reduction at the highest concentration of 100 μg/ml (Fig. 5).


Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs.

Paolini A, Curti V, Pasi F, Mazzini G, Nano R, Capelli E - Int. J. Oncol. (2015)

MicroRNA expression of miR-21-5p, miR-17-3p, miR-421-5p in T98G cells after treatment with gallic acid at different concentration. qRT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356495&req=5

f5-ijo-46-04-1491: MicroRNA expression of miR-21-5p, miR-17-3p, miR-421-5p in T98G cells after treatment with gallic acid at different concentration. qRT-PCR.
Mentions: The expression of these miRNAs was determined by qRT-PCR (see Materials and methods) and the results are reported in Fig. 5. The miRNAs considered showed a variation in their expression after GA treatment displaying a common reduced expression at low GA concentrations (1.2, 12.5 and 25 μg/ml) and an increased expression at concentrations >25 μg/ml (Fig. 5). The expression of the three different miRNAs seems to be modulated by different GA concentrations with a significant increase at the concentration of 75 μg/ml and a reduction at the highest concentration of 100 μg/ml (Fig. 5).

Bottom Line: The anticancer effect of this drug has been based on its antioxidant effects.The results confirmed in the T98G cells the anti-proliferative effect of GA reported for other glioma cell lines and showed that the miRNA expression changes depending on GA concentrations.Thus, the key for GA to induce a specific anticancer action is to use an optimal concentration that avoids these twin effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biotechnology, Laboratory of Neuro Radio Experimental Biology, 'Lazzaro Spallanzani', University of Pavia, 27100 Pavia, Italy.

ABSTRACT
Glioblastoma multiforme (GBM) is the most malignant primary brain tumor in adulthood, characterized by very high recurrence. Following the limited results for conventional therapies, novel therapeutic agents are under investigation. Among the putative new molecules, gallic acid (GA) represents a promising new anticancer drug. The anticancer effect of this drug has been based on its antioxidant effects. The aim of the present study was to investigate the toxic effects of GA on the T98G human glioblastoma cell line and its capacity to modulate the expression of microRNAs targeting the genes involved in tumor growth and invasion. Cytotoxicity, clonogenic ability and cell migration after GA treatment were tested. Moreover, the expression of miRNAs that target genes for antioxidant mitochondrial enzymes (mir-17-3p), p-21 protein (mir-21-5p) and ATM (mir-421-5p) was determined by qRT-PCR. The results confirmed in the T98G cells the anti-proliferative effect of GA reported for other glioma cell lines and showed that the miRNA expression changes depending on GA concentrations. Different GA concentrations can determine a protective or a toxic effect on tumor cells. Thus, the key for GA to induce a specific anticancer action is to use an optimal concentration that avoids these twin effects.

Show MeSH
Related in: MedlinePlus