Limits...
The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway.

Lee MK, Kim IH, Choi YH, Choi JW, Kim YM, Nam TJ - Int. J. Mol. Med. (2015)

Bottom Line: The results demonstrated an increased number of cells in the G1 phase and an enhanced cell proliferation.In addition, the upregulation of cyclin D, cyclin E, Cdk2, Cdk4 and Cdk6 was observed accompanied by a decreased in p21 and p27 expression.These findings suggest that PYP1 (1-20) stimulates the proliferation of rat IEC-6 cells by activating the EGFR signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.

ABSTRACT
For a number of years, seaweed has been used as a functional food in Asian countries, particularly in Korea, Japan and China. Pyropia yezoensis is a marine red alga that has potentially beneficial biological activities. In this study, we examined the mechanisms through which a Pyropia yezoensis peptide [PYP1 (1-20)] induces the proliferation of IEC-6 cells, a rat intestinal epithelial cell line, and the involvement of the epidermal growth factor receptor (EGFR) signaling pathway. First, cell viability assay revealed that PYP1 (1-20) induced cell proliferation in a concentration-dependent manner. Subsequently, we examined the mechanisms responsible for this induction of proliferation induced by PYP1 (1-20). EGFR is widely expressed in mammalian epithelial tissues, and the binding of this ligand affects a variety of cell physiological parameters, such as cell growth and proliferation. PYP1 (1-20) increased the expression of EGFR, Shc, growth factor receptor-bound protein 2 (Grb2) and son of sevenless (SOS). EGFR also induced the activation of the Ras signaling pathway through Raf, MEK and extracellular signal-regulated kinase (ERK) phosphorylation. In addition, cell cycle analysis revealed the expression of cell cycle-related proteins. The results demonstrated an increased number of cells in the G1 phase and an enhanced cell proliferation. In addition, the upregulation of cyclin D, cyclin E, Cdk2, Cdk4 and Cdk6 was observed accompanied by a decreased in p21 and p27 expression. These findings suggest that PYP1 (1-20) stimulates the proliferation of rat IEC-6 cells by activating the EGFR signaling pathway. Therefore, PYP1 (1-20) may be a potential source for the development of bio-functional foods which promotes the proliferation of intestinal epithelial cells.

Show MeSH
Effect of treatment with Pyropia yezoensis peptide [PYP1 (1–20)] on epidermal growth factor receptor (EGFR), Shc, growth factor receptor bound protein 2 (Grb2) and son of sevenless (SOS) protein and mRNA expression levels in IEC-6 cells. Proteins were subjected to western blot analysis and cDNA was subjected to RT-PCR. (A) Protein expression levels were increased upon incubation with PYP1 (1–20) for 24 h. (B) mRNA expression levels were also increased, as demonstrated by RT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4356455&req=5

f1-ijmm-35-04-0909: Effect of treatment with Pyropia yezoensis peptide [PYP1 (1–20)] on epidermal growth factor receptor (EGFR), Shc, growth factor receptor bound protein 2 (Grb2) and son of sevenless (SOS) protein and mRNA expression levels in IEC-6 cells. Proteins were subjected to western blot analysis and cDNA was subjected to RT-PCR. (A) Protein expression levels were increased upon incubation with PYP1 (1–20) for 24 h. (B) mRNA expression levels were also increased, as demonstrated by RT-PCR.

Mentions: To investigate the mechanisms responsible for the PYP1 (1–20)-induced proliferation of IEC-6 cells, we examined the effects of PYP1 (1–20) on EGFR signaling-related proteins. The protein and mRNA expression levels of phoshorylated (p-)EGFR, Shc, Grb2 and SOS in the IEC-6 cells treated with PYP1 (1–20) (125, 250, 500 and 1,000 ng/ml) for 24 h were measured by western blot analysis and RT-PCR. Treatment with PYP1 (1–20) upregulated the protein (Fig. 1A) and mRNA (Fig. 1B) expression levels of p-EGFR, Shc, Grb2 and SOS in a dose-dependent manner. These results indicate that PYP1 (1–20) promotes the expression of EGFR signaling-related molecules.


The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway.

Lee MK, Kim IH, Choi YH, Choi JW, Kim YM, Nam TJ - Int. J. Mol. Med. (2015)

Effect of treatment with Pyropia yezoensis peptide [PYP1 (1–20)] on epidermal growth factor receptor (EGFR), Shc, growth factor receptor bound protein 2 (Grb2) and son of sevenless (SOS) protein and mRNA expression levels in IEC-6 cells. Proteins were subjected to western blot analysis and cDNA was subjected to RT-PCR. (A) Protein expression levels were increased upon incubation with PYP1 (1–20) for 24 h. (B) mRNA expression levels were also increased, as demonstrated by RT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356455&req=5

f1-ijmm-35-04-0909: Effect of treatment with Pyropia yezoensis peptide [PYP1 (1–20)] on epidermal growth factor receptor (EGFR), Shc, growth factor receptor bound protein 2 (Grb2) and son of sevenless (SOS) protein and mRNA expression levels in IEC-6 cells. Proteins were subjected to western blot analysis and cDNA was subjected to RT-PCR. (A) Protein expression levels were increased upon incubation with PYP1 (1–20) for 24 h. (B) mRNA expression levels were also increased, as demonstrated by RT-PCR.
Mentions: To investigate the mechanisms responsible for the PYP1 (1–20)-induced proliferation of IEC-6 cells, we examined the effects of PYP1 (1–20) on EGFR signaling-related proteins. The protein and mRNA expression levels of phoshorylated (p-)EGFR, Shc, Grb2 and SOS in the IEC-6 cells treated with PYP1 (1–20) (125, 250, 500 and 1,000 ng/ml) for 24 h were measured by western blot analysis and RT-PCR. Treatment with PYP1 (1–20) upregulated the protein (Fig. 1A) and mRNA (Fig. 1B) expression levels of p-EGFR, Shc, Grb2 and SOS in a dose-dependent manner. These results indicate that PYP1 (1–20) promotes the expression of EGFR signaling-related molecules.

Bottom Line: The results demonstrated an increased number of cells in the G1 phase and an enhanced cell proliferation.In addition, the upregulation of cyclin D, cyclin E, Cdk2, Cdk4 and Cdk6 was observed accompanied by a decreased in p21 and p27 expression.These findings suggest that PYP1 (1-20) stimulates the proliferation of rat IEC-6 cells by activating the EGFR signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.

ABSTRACT
For a number of years, seaweed has been used as a functional food in Asian countries, particularly in Korea, Japan and China. Pyropia yezoensis is a marine red alga that has potentially beneficial biological activities. In this study, we examined the mechanisms through which a Pyropia yezoensis peptide [PYP1 (1-20)] induces the proliferation of IEC-6 cells, a rat intestinal epithelial cell line, and the involvement of the epidermal growth factor receptor (EGFR) signaling pathway. First, cell viability assay revealed that PYP1 (1-20) induced cell proliferation in a concentration-dependent manner. Subsequently, we examined the mechanisms responsible for this induction of proliferation induced by PYP1 (1-20). EGFR is widely expressed in mammalian epithelial tissues, and the binding of this ligand affects a variety of cell physiological parameters, such as cell growth and proliferation. PYP1 (1-20) increased the expression of EGFR, Shc, growth factor receptor-bound protein 2 (Grb2) and son of sevenless (SOS). EGFR also induced the activation of the Ras signaling pathway through Raf, MEK and extracellular signal-regulated kinase (ERK) phosphorylation. In addition, cell cycle analysis revealed the expression of cell cycle-related proteins. The results demonstrated an increased number of cells in the G1 phase and an enhanced cell proliferation. In addition, the upregulation of cyclin D, cyclin E, Cdk2, Cdk4 and Cdk6 was observed accompanied by a decreased in p21 and p27 expression. These findings suggest that PYP1 (1-20) stimulates the proliferation of rat IEC-6 cells by activating the EGFR signaling pathway. Therefore, PYP1 (1-20) may be a potential source for the development of bio-functional foods which promotes the proliferation of intestinal epithelial cells.

Show MeSH