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A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line.

Li W, Wang Y, Song Y, Xu L, Zhao J, Fang B - Oncol Lett (2015)

Bottom Line: Curcumin is an inexpensive, natural plant ingredient with protease inhibitor effects.A growth curve was constructed in order to observe the relative growth velocity, and MTT was used to analyze the effect of different concentrations of curcumin on inhibiting the proliferation of the RPMI 8226 cells.In the MM RPMI 8226 cells treated with curcumin, the expression of the p53 and Bax genes was upregulated, while the expression of the MDM2 gene was downregulated. p53 protein expression was higher in the curcumin experimental group compared with the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan, P.R. China.

ABSTRACT

Curcumin is an inexpensive, natural plant ingredient with protease inhibitor effects. The present study aimed to analyze the inhibitory effects of curcumin on the multiple myeloma (MM) RPMI 8226 cell line, and examine the underlying mechanism that promotes the apoptosis of RPMI 8226 cells. A growth curve was constructed in order to observe the relative growth velocity, and MTT was used to analyze the effect of different concentrations of curcumin on inhibiting the proliferation of the RPMI 8226 cells. The mRNA expression of the p53, Bax and MDM2 genes was detected using quantitative polymerase chain reaction. The expression of p53 protein in the MM RPMI 8226 cells following treatment with curcumin was detected by western blotting and ELISA. Curcumin inhibited the proliferation of the MM RPMI 8226 cells in a dose- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, the expression of the p53 and Bax genes was upregulated, while the expression of the MDM2 gene was downregulated. p53 protein expression was higher in the curcumin experimental group compared with the control group. Subsequent to treatment with curcumin, the growth of the MM RPMI 8226 cell line was inhibited in a concentration- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, p53 protein levels were upregulated, which suggested that curcumin may promote the apoptosis of MM cells by upregulating p53 protein expression.

No MeSH data available.


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Cells were treated with various concentrations of curcumin for 24, 48 and 72 h prior to the determination of cytotoxicity by an MTT cell proliferation assay. Each value is expressed as the mean ± standard deviation of six measurements.
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f2-ol-09-04-1719: Cells were treated with various concentrations of curcumin for 24, 48 and 72 h prior to the determination of cytotoxicity by an MTT cell proliferation assay. Each value is expressed as the mean ± standard deviation of six measurements.

Mentions: In order to investigate the effect of curcumin on the proliferation inhibition of the MM RPMI 8226 cell line, the OD was measured at 490 nm following 24, 48 and 72 h of treatment with different concentrations of curcumin. The proliferation inhibition ratio following 24, 48 and 72 h of treatment with 10 μmol/l curcumin was 17.6, 29.2 and 33.8%, respectively. The difference in the OD value was statistically significant between the experimental and control groups (P<0.05). The proliferation inhibition ratio following 24, 48 and 72 h of treatment with 15 μmol/l curcumin was 25.8, 46.1 and 50.4%, respectively. The difference in the OD value was statistically significant between the experimental and control groups (P<0.05). The results revealed that a higher concentration of curcumin was more potent than a lower concentration of curcumin at the same time-point in the growth suppression of the RPMI 8226 cells, and that a longer duration of treatment was more potent than a shorter duration of treatment with the same concentration of curcumin in the growth suppression of the RPMI 8226 cells (Fig. 2).


A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line.

Li W, Wang Y, Song Y, Xu L, Zhao J, Fang B - Oncol Lett (2015)

Cells were treated with various concentrations of curcumin for 24, 48 and 72 h prior to the determination of cytotoxicity by an MTT cell proliferation assay. Each value is expressed as the mean ± standard deviation of six measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356419&req=5

f2-ol-09-04-1719: Cells were treated with various concentrations of curcumin for 24, 48 and 72 h prior to the determination of cytotoxicity by an MTT cell proliferation assay. Each value is expressed as the mean ± standard deviation of six measurements.
Mentions: In order to investigate the effect of curcumin on the proliferation inhibition of the MM RPMI 8226 cell line, the OD was measured at 490 nm following 24, 48 and 72 h of treatment with different concentrations of curcumin. The proliferation inhibition ratio following 24, 48 and 72 h of treatment with 10 μmol/l curcumin was 17.6, 29.2 and 33.8%, respectively. The difference in the OD value was statistically significant between the experimental and control groups (P<0.05). The proliferation inhibition ratio following 24, 48 and 72 h of treatment with 15 μmol/l curcumin was 25.8, 46.1 and 50.4%, respectively. The difference in the OD value was statistically significant between the experimental and control groups (P<0.05). The results revealed that a higher concentration of curcumin was more potent than a lower concentration of curcumin at the same time-point in the growth suppression of the RPMI 8226 cells, and that a longer duration of treatment was more potent than a shorter duration of treatment with the same concentration of curcumin in the growth suppression of the RPMI 8226 cells (Fig. 2).

Bottom Line: Curcumin is an inexpensive, natural plant ingredient with protease inhibitor effects.A growth curve was constructed in order to observe the relative growth velocity, and MTT was used to analyze the effect of different concentrations of curcumin on inhibiting the proliferation of the RPMI 8226 cells.In the MM RPMI 8226 cells treated with curcumin, the expression of the p53 and Bax genes was upregulated, while the expression of the MDM2 gene was downregulated. p53 protein expression was higher in the curcumin experimental group compared with the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan, P.R. China.

ABSTRACT

Curcumin is an inexpensive, natural plant ingredient with protease inhibitor effects. The present study aimed to analyze the inhibitory effects of curcumin on the multiple myeloma (MM) RPMI 8226 cell line, and examine the underlying mechanism that promotes the apoptosis of RPMI 8226 cells. A growth curve was constructed in order to observe the relative growth velocity, and MTT was used to analyze the effect of different concentrations of curcumin on inhibiting the proliferation of the RPMI 8226 cells. The mRNA expression of the p53, Bax and MDM2 genes was detected using quantitative polymerase chain reaction. The expression of p53 protein in the MM RPMI 8226 cells following treatment with curcumin was detected by western blotting and ELISA. Curcumin inhibited the proliferation of the MM RPMI 8226 cells in a dose- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, the expression of the p53 and Bax genes was upregulated, while the expression of the MDM2 gene was downregulated. p53 protein expression was higher in the curcumin experimental group compared with the control group. Subsequent to treatment with curcumin, the growth of the MM RPMI 8226 cell line was inhibited in a concentration- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, p53 protein levels were upregulated, which suggested that curcumin may promote the apoptosis of MM cells by upregulating p53 protein expression.

No MeSH data available.


Related in: MedlinePlus