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miR-218 inhibits the migration and invasion of glioma U87 cells through the Slit2-Robo1 pathway.

Gu JJ, Gao GZ, Zhang SM - Oncol Lett (2015)

Bottom Line: Malignant gliomas are the most common primary brain tumors in adults and are associated with the highest mortality rate.Preventing the invasive behavior of malignant glioma cells by altering effector molecules can significantly improve the prognosis of a patient. microRNAs (miRNAs) are small noncoding RNAs, ~22 nucleotides in length, that are able to function as oncogenes or tumor suppressors in human cancer.In addition, it was demonstrated that miR-218 inactivated the Slit2-Robo1 pathway through downregulating Robo1 expression by directly targeting the 3'-untranslated region (3'-UTR) of Robo1.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China.

ABSTRACT

Malignant gliomas are the most common primary brain tumors in adults and are associated with the highest mortality rate. Glioma invasion is one of the most notable causes of the poor prognosis of this cancer. Preventing the invasive behavior of malignant glioma cells by altering effector molecules can significantly improve the prognosis of a patient. microRNAs (miRNAs) are small noncoding RNAs, ~22 nucleotides in length, that are able to function as oncogenes or tumor suppressors in human cancer. In the present study, the expression level of miRNA 218 (miR-218) was found to be markedly downregulated in glioma cell lines and human primary glioma tissues. miR-218 upregulation was found to dramatically reduce the migratory speed and invasive ability of glioma cells. Furthermore, it was demonstrated that ectopic expression of miR-218 in glioma cells resulted in the downregulation of roundabout, axon guidance receptor, homolog 1 (Robo1), upregulation of Slit homolog 2 (Slit2) and the expression of associated proteins following Robo1 knockdown by small interfering RNA. In addition, it was demonstrated that miR-218 inactivated the Slit2-Robo1 pathway through downregulating Robo1 expression by directly targeting the 3'-untranslated region (3'-UTR) of Robo1. The present results indicate that miR-218 plays important roles in preventing the invasiveness of glioma cells, and reveals a novel mechanism of miRNA-mediated direct suppression of the Slit2-Robo1 pathway in glioma.

No MeSH data available.


Related in: MedlinePlus

In the U87 cell line, Robo1 siRNA reduced miR-218 inhibitor-induced enhanced invasive ability. (A and B) Transwell assays revealed that the miR-218 inhibitor promoted the invasion of U87 cells and Robo1 siRNA attenuated the invasion. *P<0.05. (C and D) Robo1 siRNA attenuation of the migration of U87 cells was also observed in the scratch-wound assay. *P<0.05. NC, negative control; siRNA, small interfering RNA; Robo1, roundabout, axon guidance receptor, homolog 1; miR-218, microRNA 218.
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f6-ol-09-04-1561: In the U87 cell line, Robo1 siRNA reduced miR-218 inhibitor-induced enhanced invasive ability. (A and B) Transwell assays revealed that the miR-218 inhibitor promoted the invasion of U87 cells and Robo1 siRNA attenuated the invasion. *P<0.05. (C and D) Robo1 siRNA attenuation of the migration of U87 cells was also observed in the scratch-wound assay. *P<0.05. NC, negative control; siRNA, small interfering RNA; Robo1, roundabout, axon guidance receptor, homolog 1; miR-218, microRNA 218.

Mentions: To assess the role of Robo1 in the miR-218-dependent inhibition of cell migration and invasion, miR-218 inhibitor was transfected into U87 cells treated with Robo1 siRNA. As expected, Robo1 protein expression was significantly reduced by the specific Robo1 siRNA (Fig. 5). The enhanced invasive ability of miR-218 inhibitor-transfected U87 cells declined when Robo1 siRNA was co-transfected with the miR-218 inhibitor (Fig. 6). These results indicate that Robo1 is essential for miR-218-dependent cell migration and invasion.


miR-218 inhibits the migration and invasion of glioma U87 cells through the Slit2-Robo1 pathway.

Gu JJ, Gao GZ, Zhang SM - Oncol Lett (2015)

In the U87 cell line, Robo1 siRNA reduced miR-218 inhibitor-induced enhanced invasive ability. (A and B) Transwell assays revealed that the miR-218 inhibitor promoted the invasion of U87 cells and Robo1 siRNA attenuated the invasion. *P<0.05. (C and D) Robo1 siRNA attenuation of the migration of U87 cells was also observed in the scratch-wound assay. *P<0.05. NC, negative control; siRNA, small interfering RNA; Robo1, roundabout, axon guidance receptor, homolog 1; miR-218, microRNA 218.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356403&req=5

f6-ol-09-04-1561: In the U87 cell line, Robo1 siRNA reduced miR-218 inhibitor-induced enhanced invasive ability. (A and B) Transwell assays revealed that the miR-218 inhibitor promoted the invasion of U87 cells and Robo1 siRNA attenuated the invasion. *P<0.05. (C and D) Robo1 siRNA attenuation of the migration of U87 cells was also observed in the scratch-wound assay. *P<0.05. NC, negative control; siRNA, small interfering RNA; Robo1, roundabout, axon guidance receptor, homolog 1; miR-218, microRNA 218.
Mentions: To assess the role of Robo1 in the miR-218-dependent inhibition of cell migration and invasion, miR-218 inhibitor was transfected into U87 cells treated with Robo1 siRNA. As expected, Robo1 protein expression was significantly reduced by the specific Robo1 siRNA (Fig. 5). The enhanced invasive ability of miR-218 inhibitor-transfected U87 cells declined when Robo1 siRNA was co-transfected with the miR-218 inhibitor (Fig. 6). These results indicate that Robo1 is essential for miR-218-dependent cell migration and invasion.

Bottom Line: Malignant gliomas are the most common primary brain tumors in adults and are associated with the highest mortality rate.Preventing the invasive behavior of malignant glioma cells by altering effector molecules can significantly improve the prognosis of a patient. microRNAs (miRNAs) are small noncoding RNAs, ~22 nucleotides in length, that are able to function as oncogenes or tumor suppressors in human cancer.In addition, it was demonstrated that miR-218 inactivated the Slit2-Robo1 pathway through downregulating Robo1 expression by directly targeting the 3'-untranslated region (3'-UTR) of Robo1.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China.

ABSTRACT

Malignant gliomas are the most common primary brain tumors in adults and are associated with the highest mortality rate. Glioma invasion is one of the most notable causes of the poor prognosis of this cancer. Preventing the invasive behavior of malignant glioma cells by altering effector molecules can significantly improve the prognosis of a patient. microRNAs (miRNAs) are small noncoding RNAs, ~22 nucleotides in length, that are able to function as oncogenes or tumor suppressors in human cancer. In the present study, the expression level of miRNA 218 (miR-218) was found to be markedly downregulated in glioma cell lines and human primary glioma tissues. miR-218 upregulation was found to dramatically reduce the migratory speed and invasive ability of glioma cells. Furthermore, it was demonstrated that ectopic expression of miR-218 in glioma cells resulted in the downregulation of roundabout, axon guidance receptor, homolog 1 (Robo1), upregulation of Slit homolog 2 (Slit2) and the expression of associated proteins following Robo1 knockdown by small interfering RNA. In addition, it was demonstrated that miR-218 inactivated the Slit2-Robo1 pathway through downregulating Robo1 expression by directly targeting the 3'-untranslated region (3'-UTR) of Robo1. The present results indicate that miR-218 plays important roles in preventing the invasiveness of glioma cells, and reveals a novel mechanism of miRNA-mediated direct suppression of the Slit2-Robo1 pathway in glioma.

No MeSH data available.


Related in: MedlinePlus