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Indirect computed tomography lymphography identifies lymph node metastasis in rabbit pyriform sinus VX2 carcinoma.

Shen NA, Xu X, Sha Y, Wu H - Oncol Lett (2015)

Bottom Line: The metastasis rates of deep cervical lymph nodes were 100% in all three groups on CT-LG.The CT attenuation value of CT-LG reached peak values of 400 and 600 Hu at 1 and 3 min after the injection, which then decreased gradually.In this study, CT-LG could demonstrate the internal architecture of lymph nodes and their lymphatic vessels, and therefore may have the advantages of radiological methods such as B ultrasound, CT, magnetic resonance imaging and positron emission tomography.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.

ABSTRACT

Indirect computed tomography lymphography (CT-LG) could be used to determine the regional spread of cancer and assess lymphatic function by the interstitial delivery of diagnostic agents. Few studies have been reported on its use in pyriform sinus carcinoma. The aim of the present study was to establish the rabbit VX2 tumor as a model for pyriform sinus carcinoma and to observe its neck lymph node metastasis by indirect CT-LG. VX2 tumor tissue suspension was transplanted into the pyriform sinus submucosa of 15 rabbits under direct laryngoscope. Rabbits were randomly placed into one of three groups, each comprised of five rabbits. Observation of the tumor growth and neck lymph node metastases were taken on days 14 (group 1), 21 (group 2) and 28 (group 3) following transplantation using the method of indirect CT-LG. VX2 tumors were transplanted successfully in all rabbits. Deep cervical lymph nodes were enhanced clearly in indirect CT-LG. The contrast agent filling defected appeared on the metastasis nodes while the lymph node without metastasis was smooth. The metastasis rates of deep cervical lymph nodes were 100% in all three groups on CT-LG. The CT attenuation value of CT-LG reached peak values of 400 and 600 Hu at 1 and 3 min after the injection, which then decreased gradually. In this study, CT-LG could demonstrate the internal architecture of lymph nodes and their lymphatic vessels, and therefore may have the advantages of radiological methods such as B ultrasound, CT, magnetic resonance imaging and positron emission tomography.

No MeSH data available.


Related in: MedlinePlus

Pathological and imaging findings. (A) Blue-stained nodes, one for each side. (B) Tumors were visible in the pyriform sinus at the primary site (arrow). (C) The diameter of the lymph node was measured in the computed tomography image. (D) Lymph node metastasis of poorly differentiated squamous cell carcinoma. (hematoxylin and eosin; magnification, ×200).
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f3-ol-09-04-1802: Pathological and imaging findings. (A) Blue-stained nodes, one for each side. (B) Tumors were visible in the pyriform sinus at the primary site (arrow). (C) The diameter of the lymph node was measured in the computed tomography image. (D) Lymph node metastasis of poorly differentiated squamous cell carcinoma. (hematoxylin and eosin; magnification, ×200).

Mentions: Deep cervical lymph nodes were located laterally, with 1–2 oval lymph nodes per side, into the larynx and cricoid cartilage below the sterno-thyroid muscle. However, the blue-stain nodes were only one for each side (Fig. 3A). Following a subsequent CT scan, the nodes were observed to be enhanced. The other two groups of nodes, including the para-tracheal and sub-mandibular lymph nodes, could not be observed with the blue-stain. The occurrence of the ipsilaterally located deep lymph node metastases was observed in 100% of the animals in all the groups. Contralateral metastasis rates were 80% on day 14, 60% on day 21 and 100% on day 28 (Table I). It was in accordance with the CT image. The median diameter of the nodes on the CT images was 0.533±0.056 cm, while 0.541±0.066 cm by the histological method. No significant differences were found between histology and CT scanning (Fig. 3C).


Indirect computed tomography lymphography identifies lymph node metastasis in rabbit pyriform sinus VX2 carcinoma.

Shen NA, Xu X, Sha Y, Wu H - Oncol Lett (2015)

Pathological and imaging findings. (A) Blue-stained nodes, one for each side. (B) Tumors were visible in the pyriform sinus at the primary site (arrow). (C) The diameter of the lymph node was measured in the computed tomography image. (D) Lymph node metastasis of poorly differentiated squamous cell carcinoma. (hematoxylin and eosin; magnification, ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356291&req=5

f3-ol-09-04-1802: Pathological and imaging findings. (A) Blue-stained nodes, one for each side. (B) Tumors were visible in the pyriform sinus at the primary site (arrow). (C) The diameter of the lymph node was measured in the computed tomography image. (D) Lymph node metastasis of poorly differentiated squamous cell carcinoma. (hematoxylin and eosin; magnification, ×200).
Mentions: Deep cervical lymph nodes were located laterally, with 1–2 oval lymph nodes per side, into the larynx and cricoid cartilage below the sterno-thyroid muscle. However, the blue-stain nodes were only one for each side (Fig. 3A). Following a subsequent CT scan, the nodes were observed to be enhanced. The other two groups of nodes, including the para-tracheal and sub-mandibular lymph nodes, could not be observed with the blue-stain. The occurrence of the ipsilaterally located deep lymph node metastases was observed in 100% of the animals in all the groups. Contralateral metastasis rates were 80% on day 14, 60% on day 21 and 100% on day 28 (Table I). It was in accordance with the CT image. The median diameter of the nodes on the CT images was 0.533±0.056 cm, while 0.541±0.066 cm by the histological method. No significant differences were found between histology and CT scanning (Fig. 3C).

Bottom Line: The metastasis rates of deep cervical lymph nodes were 100% in all three groups on CT-LG.The CT attenuation value of CT-LG reached peak values of 400 and 600 Hu at 1 and 3 min after the injection, which then decreased gradually.In this study, CT-LG could demonstrate the internal architecture of lymph nodes and their lymphatic vessels, and therefore may have the advantages of radiological methods such as B ultrasound, CT, magnetic resonance imaging and positron emission tomography.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.

ABSTRACT

Indirect computed tomography lymphography (CT-LG) could be used to determine the regional spread of cancer and assess lymphatic function by the interstitial delivery of diagnostic agents. Few studies have been reported on its use in pyriform sinus carcinoma. The aim of the present study was to establish the rabbit VX2 tumor as a model for pyriform sinus carcinoma and to observe its neck lymph node metastasis by indirect CT-LG. VX2 tumor tissue suspension was transplanted into the pyriform sinus submucosa of 15 rabbits under direct laryngoscope. Rabbits were randomly placed into one of three groups, each comprised of five rabbits. Observation of the tumor growth and neck lymph node metastases were taken on days 14 (group 1), 21 (group 2) and 28 (group 3) following transplantation using the method of indirect CT-LG. VX2 tumors were transplanted successfully in all rabbits. Deep cervical lymph nodes were enhanced clearly in indirect CT-LG. The contrast agent filling defected appeared on the metastasis nodes while the lymph node without metastasis was smooth. The metastasis rates of deep cervical lymph nodes were 100% in all three groups on CT-LG. The CT attenuation value of CT-LG reached peak values of 400 and 600 Hu at 1 and 3 min after the injection, which then decreased gradually. In this study, CT-LG could demonstrate the internal architecture of lymph nodes and their lymphatic vessels, and therefore may have the advantages of radiological methods such as B ultrasound, CT, magnetic resonance imaging and positron emission tomography.

No MeSH data available.


Related in: MedlinePlus