Limits...
Phenotype and transcriptome analysis reveals chloroplast development and pigment biosynthesis together influenced the leaf color formation in mutants of Anthurium andraeanum 'Sonate'.

Yang Y, Chen X, Xu B, Li Y, Ma Y, Wang G - Front Plant Sci (2015)

Bottom Line: Knowledge of mechanisms in anthuriums to produce leaves with different shades of green would help to effectively select desirable traits.From the 27,539 (67.1%) unigenes with annotated functions, 858 significantly differently expressed genes (DEGs) were identified, consisting of 446 up-regulated genes and 412 down-regulated genes.And the possible formation pathway of leaf color mutant of A. andraeanum 'Sonate' is deduced based on our results.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Nanjing Agricultural University Nanjing, China.

ABSTRACT
Leaf color is one of the well-sought traits in breeding program for Anthurium andraeanum Lind. Knowledge of mechanisms in anthuriums to produce leaves with different shades of green would help to effectively select desirable traits. In this study, the micro- and ultra-structural and physiological features of leaves on wild type and leaf color mutants (dark green, rubescent, etiolated, albino) in A. andraeanum 'Sonate' were analyzed. Results show that chloroplasts of leaf color mutants exhibited abnormal morphology and distribution. Using next generation sequencing technology followed by de novo assembly, leaf transcriptomes comprising of 41,017 unigenes with an average sequence length of 768 bp were produced from wild type and rubescent mutant. From the 27,539 (67.1%) unigenes with annotated functions, 858 significantly differently expressed genes (DEGs) were identified, consisting of 446 up-regulated genes and 412 down-regulated genes. Genes that affect chloroplasts development and division, and chlorophyll biosynthesis were included in the down-regulated DEGs. Quantitative real-time PCR (qRT-PCR) analysis validated that the expression level of those genes was significantly lower in the rubescent, etiolated, and albino mutant compared to wild type plants, which concurs with the differences in micro- and ultra-structures and physiological features between these two types of plants. Conclusively, the leaf color formation is greatly affected by the activity of chloroplast development and pigment biosynthesis. And the possible formation pathway of leaf color mutant of A. andraeanum 'Sonate' is deduced based on our results.

No MeSH data available.


Related in: MedlinePlus

The numbers of specific genes and shared genes between the wild type and rubescent mutants of Anthurium andraeanum ‘Sonate’.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4356079&req=5

Figure 6: The numbers of specific genes and shared genes between the wild type and rubescent mutants of Anthurium andraeanum ‘Sonate’.

Mentions: The FPKM and RPKM methods were used to analyze the gene expression in the two libraries. As a result, 37,199 and 39,302 unigenes were identified respectively in the cDNA library from wild type and rubescent mutant leaves, of which 1387 and 3490 genes expressed specifically in leaves of the wild type and rubescen mutant, respectively (Figure 6). When comparing the two libraries according to RPKM calculation, a total of 858 differently expressed genes (DEGs) were detected, including 446 up-regulated genes and 412 down-regulated genes. After analysis on all DEGs, we found that more than 50% of the DEGs did not have homologous sequences meeting the search criteria in the NCBI database. These unmatched unigene sequences may contain fragmented RNAs from untranslated regions (UTRs), long non-coding RNAs (ncRNAs) and other types of transcripts, or they are the specific genes in A. andraeanum.


Phenotype and transcriptome analysis reveals chloroplast development and pigment biosynthesis together influenced the leaf color formation in mutants of Anthurium andraeanum 'Sonate'.

Yang Y, Chen X, Xu B, Li Y, Ma Y, Wang G - Front Plant Sci (2015)

The numbers of specific genes and shared genes between the wild type and rubescent mutants of Anthurium andraeanum ‘Sonate’.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4356079&req=5

Figure 6: The numbers of specific genes and shared genes between the wild type and rubescent mutants of Anthurium andraeanum ‘Sonate’.
Mentions: The FPKM and RPKM methods were used to analyze the gene expression in the two libraries. As a result, 37,199 and 39,302 unigenes were identified respectively in the cDNA library from wild type and rubescent mutant leaves, of which 1387 and 3490 genes expressed specifically in leaves of the wild type and rubescen mutant, respectively (Figure 6). When comparing the two libraries according to RPKM calculation, a total of 858 differently expressed genes (DEGs) were detected, including 446 up-regulated genes and 412 down-regulated genes. After analysis on all DEGs, we found that more than 50% of the DEGs did not have homologous sequences meeting the search criteria in the NCBI database. These unmatched unigene sequences may contain fragmented RNAs from untranslated regions (UTRs), long non-coding RNAs (ncRNAs) and other types of transcripts, or they are the specific genes in A. andraeanum.

Bottom Line: Knowledge of mechanisms in anthuriums to produce leaves with different shades of green would help to effectively select desirable traits.From the 27,539 (67.1%) unigenes with annotated functions, 858 significantly differently expressed genes (DEGs) were identified, consisting of 446 up-regulated genes and 412 down-regulated genes.And the possible formation pathway of leaf color mutant of A. andraeanum 'Sonate' is deduced based on our results.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Nanjing Agricultural University Nanjing, China.

ABSTRACT
Leaf color is one of the well-sought traits in breeding program for Anthurium andraeanum Lind. Knowledge of mechanisms in anthuriums to produce leaves with different shades of green would help to effectively select desirable traits. In this study, the micro- and ultra-structural and physiological features of leaves on wild type and leaf color mutants (dark green, rubescent, etiolated, albino) in A. andraeanum 'Sonate' were analyzed. Results show that chloroplasts of leaf color mutants exhibited abnormal morphology and distribution. Using next generation sequencing technology followed by de novo assembly, leaf transcriptomes comprising of 41,017 unigenes with an average sequence length of 768 bp were produced from wild type and rubescent mutant. From the 27,539 (67.1%) unigenes with annotated functions, 858 significantly differently expressed genes (DEGs) were identified, consisting of 446 up-regulated genes and 412 down-regulated genes. Genes that affect chloroplasts development and division, and chlorophyll biosynthesis were included in the down-regulated DEGs. Quantitative real-time PCR (qRT-PCR) analysis validated that the expression level of those genes was significantly lower in the rubescent, etiolated, and albino mutant compared to wild type plants, which concurs with the differences in micro- and ultra-structures and physiological features between these two types of plants. Conclusively, the leaf color formation is greatly affected by the activity of chloroplast development and pigment biosynthesis. And the possible formation pathway of leaf color mutant of A. andraeanum 'Sonate' is deduced based on our results.

No MeSH data available.


Related in: MedlinePlus