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Activation of κ-opioid receptor by U50,488H improves vascular dysfunction in streptozotocin-induced diabetic rats.

Zhou X, Wang D, Zhang Y, Zhang J, Xiang D, Wang H - BMC Endocr Disord (2015)

Bottom Line: U50,488H treatment resulted in reduction in ANG II, sICAM-1, IL-6 and IL-8 levels and elevation in NO levels, while these effects were abolished by nor-BNI treatment.Further more, eNOS phosphorylation was increased, and NF-κB p65 translocation was decreased after U50,488H treatment.Our study demonstrated that U50,488H may have therapeutic effects on diabetic vascular dysfunction by improving endothelial dysfunction and attenuating chronic inflammation, which may be dependent on phosphorylation of eNOS and downstream inhibition of NF-кB.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. zhouxuanxuan2007@126.com.

ABSTRACT

Background: Evidence suggests that activation of κ-opioid receptor (KOR) by U50,488H exhibits potential cardiovascular protective properties. However, the effects of U50,488H on vascular dysfunction in diabetes mellitus (DM) are still not clear. The present study was designed to investigate the effects of U50,488H on vascular dysfunction in diabetic rats and explore the underlying mechanisms involved.

Methods: Rats were randomly divided into control, DM, DM + vehicle, DM + U50,488H and DM + nor-binaltorphimine (nor-BNI) groups. Streptozotocin injection was used to induce DM. Weight, blood glucose, blood pressure and plasma insulin for each group were measured. Arterial functions were assessed with isolated vessels mounted for isometric tension recordings. Angiotensin II (ANG II), soluble intercellular adhesion molecule-1 (sICAM-1), interleukin (IL)-6 and IL-8 levels were measured by ELISA, and endothelial nitric oxide synthase (eNOS) phosphorylation and NF-κB p65 translocation were measured by Western blot.

Results: Activation of KOR by U50,488H reduced the enhanced contractility of aortas to KCl and noradrenaline and increased acetylcholine-induced vascular relaxation, which could also protect the aortal ultrastructure in DM. U50,488H treatment resulted in reduction in ANG II, sICAM-1, IL-6 and IL-8 levels and elevation in NO levels, while these effects were abolished by nor-BNI treatment. Further more, eNOS phosphorylation was increased, and NF-κB p65 translocation was decreased after U50,488H treatment.

Conclusions: Our study demonstrated that U50,488H may have therapeutic effects on diabetic vascular dysfunction by improving endothelial dysfunction and attenuating chronic inflammation, which may be dependent on phosphorylation of eNOS and downstream inhibition of NF-кB.

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KOR was expressed in thoracic aortas. (A) Expression of KOR by immunohistochemical staining. KOR was mainly detectable in the endothelium and smooth muscle of thoracic aortas, highlighted with arrows. (scale bar: 25 μm). (B) Western blot analysis for KOR protein expression. *P < 0.05 vs. CON group.
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Fig1: KOR was expressed in thoracic aortas. (A) Expression of KOR by immunohistochemical staining. KOR was mainly detectable in the endothelium and smooth muscle of thoracic aortas, highlighted with arrows. (scale bar: 25 μm). (B) Western blot analysis for KOR protein expression. *P < 0.05 vs. CON group.

Mentions: As shown in Figure 1A, KOR was mainly detectable in the endothelium and smooth muscle of the thoracic aortas. Compared with the CON group, the KOR protein expression increased significantly in the DM group, which was also confirmed by Western blot (Figure 1B). We further tested KOR expression in the DM + U50,488H group and found no statistically significant difference compared with the DM group (Figure 1C).Figure 1


Activation of κ-opioid receptor by U50,488H improves vascular dysfunction in streptozotocin-induced diabetic rats.

Zhou X, Wang D, Zhang Y, Zhang J, Xiang D, Wang H - BMC Endocr Disord (2015)

KOR was expressed in thoracic aortas. (A) Expression of KOR by immunohistochemical staining. KOR was mainly detectable in the endothelium and smooth muscle of thoracic aortas, highlighted with arrows. (scale bar: 25 μm). (B) Western blot analysis for KOR protein expression. *P < 0.05 vs. CON group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4355970&req=5

Fig1: KOR was expressed in thoracic aortas. (A) Expression of KOR by immunohistochemical staining. KOR was mainly detectable in the endothelium and smooth muscle of thoracic aortas, highlighted with arrows. (scale bar: 25 μm). (B) Western blot analysis for KOR protein expression. *P < 0.05 vs. CON group.
Mentions: As shown in Figure 1A, KOR was mainly detectable in the endothelium and smooth muscle of the thoracic aortas. Compared with the CON group, the KOR protein expression increased significantly in the DM group, which was also confirmed by Western blot (Figure 1B). We further tested KOR expression in the DM + U50,488H group and found no statistically significant difference compared with the DM group (Figure 1C).Figure 1

Bottom Line: U50,488H treatment resulted in reduction in ANG II, sICAM-1, IL-6 and IL-8 levels and elevation in NO levels, while these effects were abolished by nor-BNI treatment.Further more, eNOS phosphorylation was increased, and NF-κB p65 translocation was decreased after U50,488H treatment.Our study demonstrated that U50,488H may have therapeutic effects on diabetic vascular dysfunction by improving endothelial dysfunction and attenuating chronic inflammation, which may be dependent on phosphorylation of eNOS and downstream inhibition of NF-кB.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. zhouxuanxuan2007@126.com.

ABSTRACT

Background: Evidence suggests that activation of κ-opioid receptor (KOR) by U50,488H exhibits potential cardiovascular protective properties. However, the effects of U50,488H on vascular dysfunction in diabetes mellitus (DM) are still not clear. The present study was designed to investigate the effects of U50,488H on vascular dysfunction in diabetic rats and explore the underlying mechanisms involved.

Methods: Rats were randomly divided into control, DM, DM + vehicle, DM + U50,488H and DM + nor-binaltorphimine (nor-BNI) groups. Streptozotocin injection was used to induce DM. Weight, blood glucose, blood pressure and plasma insulin for each group were measured. Arterial functions were assessed with isolated vessels mounted for isometric tension recordings. Angiotensin II (ANG II), soluble intercellular adhesion molecule-1 (sICAM-1), interleukin (IL)-6 and IL-8 levels were measured by ELISA, and endothelial nitric oxide synthase (eNOS) phosphorylation and NF-κB p65 translocation were measured by Western blot.

Results: Activation of KOR by U50,488H reduced the enhanced contractility of aortas to KCl and noradrenaline and increased acetylcholine-induced vascular relaxation, which could also protect the aortal ultrastructure in DM. U50,488H treatment resulted in reduction in ANG II, sICAM-1, IL-6 and IL-8 levels and elevation in NO levels, while these effects were abolished by nor-BNI treatment. Further more, eNOS phosphorylation was increased, and NF-κB p65 translocation was decreased after U50,488H treatment.

Conclusions: Our study demonstrated that U50,488H may have therapeutic effects on diabetic vascular dysfunction by improving endothelial dysfunction and attenuating chronic inflammation, which may be dependent on phosphorylation of eNOS and downstream inhibition of NF-кB.

Show MeSH
Related in: MedlinePlus