Limits...
16S gut community of the Cameron County Hispanic Cohort.

Ross MC, Muzny DM, McCormick JB, Gibbs RA, Fisher-Hoch SP, Petrosino JF - Microbiome (2015)

Bottom Line: We found that these communities, when compared to Human Microbiome Project subjects, exhibit community shifts often observed in obese and T2D individuals in published studies.We identified a group of seven genera that form a tightly interconnected network present in all four tested datasets, dominated by butyrate producers, which are often increased in obese individuals while being depleted in T2D patients.The lack of CCHC subject gut community segregation based on all tested metadata suggests that the community structure we observe in the CCHC likely occurs early in life, and endures.

View Article: PubMed Central - PubMed

Affiliation: Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, TX USA ; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX USA.

ABSTRACT

Background: Obesity and type 2 diabetes (T2D) are major public health concerns worldwide, and their prevalence has only increased in recent years. Mexican Americans are disproportionately afflicted by obesity and T2D, and rates are even higher in the United States-Mexico border region. To determine the factors associated with the increased risk of T2D, obesity, and other diseases in this population, the Cameron County Hispanic Cohort was established in 2004.

Results: In this study, we characterized the 16S gut community of a subset of 63 subjects from this unique cohort. We found that these communities, when compared to Human Microbiome Project subjects, exhibit community shifts often observed in obese and T2D individuals in published studies. We also examined microbial network relationships between operational taxonomic units (OTUs) in the Cameron County Hispanic Cohort (CCHC) and three additional datasets. We identified a group of seven genera that form a tightly interconnected network present in all four tested datasets, dominated by butyrate producers, which are often increased in obese individuals while being depleted in T2D patients.

Conclusions: Through a combination of increased disease prevalence and relatively high gut microbial homogeneity in the subset of CCHC members we examined, we believe that the CCHC may represent an ideal community to dissect mechanisms underlying the role of the gut microbiome in human health and disease. The lack of CCHC subject gut community segregation based on all tested metadata suggests that the community structure we observe in the CCHC likely occurs early in life, and endures. This persistent 'disease'-related gut microbial community in CCHC subjects may enhance existing genetic or lifestyle predispositions to the prevalent diseases of the CCHC, leading to increased attack rates of obesity, T2D, non-alcoholic fatty liver disease, and others.

No MeSH data available.


Related in: MedlinePlus

16S rRNA sequence principle coordinates analysis (PCoA) plots of CCHC and HMP stool samples. (A) Comparison of stool samples from the CCHC and HMP analyzed using the unweighted UniFrac metric. (B) Same stool samples as in 1A analyzed using the weighted UniFrac metric; HMP n = 213, CCHC n = 63. CCHC, Cameron County Hispanic Cohort; HMP, Human Microbiome Project; PC, principal coordinate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4355967&req=5

Fig2: 16S rRNA sequence principle coordinates analysis (PCoA) plots of CCHC and HMP stool samples. (A) Comparison of stool samples from the CCHC and HMP analyzed using the unweighted UniFrac metric. (B) Same stool samples as in 1A analyzed using the weighted UniFrac metric; HMP n = 213, CCHC n = 63. CCHC, Cameron County Hispanic Cohort; HMP, Human Microbiome Project; PC, principal coordinate.

Mentions: The US-Mexico border region comprises a diverse mixture of economies and disease burdens owing to the very unique countries that lie on either side. Driving this dichotomy is the greater than fivefold disparity between the GDP-per-capita of these two countries [34]. To identify the important risk factors for obesity and T2D of Mexican Americans living in the lower Rio Grande Valley, the Cameron County Hispanic Cohort (CCHC) was established in 2004 [35] (Figure 1). Overall, members of this community have much higher obesity (50.9% BMI ≥30, 9.0% BMI ≥40) and T2D rates (28.0%) versus the average American population (35.7% BMI ≥30, 6.3% BMI ≥40, T2D 8.3%) [36-38]. These statistics reflect the general trend of Mexican Americans living along the entire US-Mexico border [27]. Participants in this study were slightly heavier but had lower T2D rates than the CCHC as a whole (60% BMI ≥30, 12.9% T2D). The CCHC represents the first exclusively Mexican American group from a border city with poor overall health. In light of the previously described recent studies, and the disproportionately increased prevalence of T2D and obesity in this population, we sought to characterize the gut microbiome in 63 subjects belonging to the CCHC. For this study, we chose to utilize Human Microbiome Project (HMP) stool data for comparative analysis [39-41] (Figure 2) (Figure 3). The 300 participants of the HMP were subjected to a lengthy list of exclusion criteria and here represent a healthy Western microbiome [42]. Using the HMP stool data as a reference will help determine associations with the gut microbial structure and increase our understanding about the observed predisposition to obesity and T2D in the CCHC.Figure 1


16S gut community of the Cameron County Hispanic Cohort.

Ross MC, Muzny DM, McCormick JB, Gibbs RA, Fisher-Hoch SP, Petrosino JF - Microbiome (2015)

16S rRNA sequence principle coordinates analysis (PCoA) plots of CCHC and HMP stool samples. (A) Comparison of stool samples from the CCHC and HMP analyzed using the unweighted UniFrac metric. (B) Same stool samples as in 1A analyzed using the weighted UniFrac metric; HMP n = 213, CCHC n = 63. CCHC, Cameron County Hispanic Cohort; HMP, Human Microbiome Project; PC, principal coordinate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4355967&req=5

Fig2: 16S rRNA sequence principle coordinates analysis (PCoA) plots of CCHC and HMP stool samples. (A) Comparison of stool samples from the CCHC and HMP analyzed using the unweighted UniFrac metric. (B) Same stool samples as in 1A analyzed using the weighted UniFrac metric; HMP n = 213, CCHC n = 63. CCHC, Cameron County Hispanic Cohort; HMP, Human Microbiome Project; PC, principal coordinate.
Mentions: The US-Mexico border region comprises a diverse mixture of economies and disease burdens owing to the very unique countries that lie on either side. Driving this dichotomy is the greater than fivefold disparity between the GDP-per-capita of these two countries [34]. To identify the important risk factors for obesity and T2D of Mexican Americans living in the lower Rio Grande Valley, the Cameron County Hispanic Cohort (CCHC) was established in 2004 [35] (Figure 1). Overall, members of this community have much higher obesity (50.9% BMI ≥30, 9.0% BMI ≥40) and T2D rates (28.0%) versus the average American population (35.7% BMI ≥30, 6.3% BMI ≥40, T2D 8.3%) [36-38]. These statistics reflect the general trend of Mexican Americans living along the entire US-Mexico border [27]. Participants in this study were slightly heavier but had lower T2D rates than the CCHC as a whole (60% BMI ≥30, 12.9% T2D). The CCHC represents the first exclusively Mexican American group from a border city with poor overall health. In light of the previously described recent studies, and the disproportionately increased prevalence of T2D and obesity in this population, we sought to characterize the gut microbiome in 63 subjects belonging to the CCHC. For this study, we chose to utilize Human Microbiome Project (HMP) stool data for comparative analysis [39-41] (Figure 2) (Figure 3). The 300 participants of the HMP were subjected to a lengthy list of exclusion criteria and here represent a healthy Western microbiome [42]. Using the HMP stool data as a reference will help determine associations with the gut microbial structure and increase our understanding about the observed predisposition to obesity and T2D in the CCHC.Figure 1

Bottom Line: We found that these communities, when compared to Human Microbiome Project subjects, exhibit community shifts often observed in obese and T2D individuals in published studies.We identified a group of seven genera that form a tightly interconnected network present in all four tested datasets, dominated by butyrate producers, which are often increased in obese individuals while being depleted in T2D patients.The lack of CCHC subject gut community segregation based on all tested metadata suggests that the community structure we observe in the CCHC likely occurs early in life, and endures.

View Article: PubMed Central - PubMed

Affiliation: Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, TX USA ; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX USA.

ABSTRACT

Background: Obesity and type 2 diabetes (T2D) are major public health concerns worldwide, and their prevalence has only increased in recent years. Mexican Americans are disproportionately afflicted by obesity and T2D, and rates are even higher in the United States-Mexico border region. To determine the factors associated with the increased risk of T2D, obesity, and other diseases in this population, the Cameron County Hispanic Cohort was established in 2004.

Results: In this study, we characterized the 16S gut community of a subset of 63 subjects from this unique cohort. We found that these communities, when compared to Human Microbiome Project subjects, exhibit community shifts often observed in obese and T2D individuals in published studies. We also examined microbial network relationships between operational taxonomic units (OTUs) in the Cameron County Hispanic Cohort (CCHC) and three additional datasets. We identified a group of seven genera that form a tightly interconnected network present in all four tested datasets, dominated by butyrate producers, which are often increased in obese individuals while being depleted in T2D patients.

Conclusions: Through a combination of increased disease prevalence and relatively high gut microbial homogeneity in the subset of CCHC members we examined, we believe that the CCHC may represent an ideal community to dissect mechanisms underlying the role of the gut microbiome in human health and disease. The lack of CCHC subject gut community segregation based on all tested metadata suggests that the community structure we observe in the CCHC likely occurs early in life, and endures. This persistent 'disease'-related gut microbial community in CCHC subjects may enhance existing genetic or lifestyle predispositions to the prevalent diseases of the CCHC, leading to increased attack rates of obesity, T2D, non-alcoholic fatty liver disease, and others.

No MeSH data available.


Related in: MedlinePlus