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Separation and Identification of Phenolic Acid and Flavonoids from Nerium indicum Flowers.

Vinayagam A, Sudha PN - Indian J Pharm Sci (2015 Jan-Feb)

Bottom Line: Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4).In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV.The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Sathyabama University, Chennai-600 119, India.

ABSTRACT
Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4). In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV. The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.

No MeSH data available.


Related in: MedlinePlus

TIC/MS chromatogram of cis and trans isomer of 5-O-caffeoyl quinic acid from HPLC-(-) ESI-MS.Cis isomer with m/z value of 362.9 and trans isomer with m/z value of 363.0.
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Figure 4: TIC/MS chromatogram of cis and trans isomer of 5-O-caffeoyl quinic acid from HPLC-(-) ESI-MS.Cis isomer with m/z value of 362.9 and trans isomer with m/z value of 363.0.

Mentions: Compound 1 observed as a [M-H] - ion peak at m/z 353 in the ESI mass spectrum (fig. 2) and based on literature data[23] compound 1 was confirmed as a 5-O-caffeoyl quinic acid. By using Zorbax SB Phenyl column able to separate cis and trans isomer of 5-O-caffeoyl quinic acid (fig. 3) gave an [M-H]-ion peak at m/z 353 in the ESI mass spectrum (fig. 4). UV spectra also suggest that trans isomer is more absorption value compared to cis isomer (fig. 5). Mass spectral value of compound 2 showed a quasi-molecular ion [M-H] - at m/z 609, compared with literature data[24] compound 2 was identified as a quercetin-3-O-rutinoside (fig. 2).


Separation and Identification of Phenolic Acid and Flavonoids from Nerium indicum Flowers.

Vinayagam A, Sudha PN - Indian J Pharm Sci (2015 Jan-Feb)

TIC/MS chromatogram of cis and trans isomer of 5-O-caffeoyl quinic acid from HPLC-(-) ESI-MS.Cis isomer with m/z value of 362.9 and trans isomer with m/z value of 363.0.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355888&req=5

Figure 4: TIC/MS chromatogram of cis and trans isomer of 5-O-caffeoyl quinic acid from HPLC-(-) ESI-MS.Cis isomer with m/z value of 362.9 and trans isomer with m/z value of 363.0.
Mentions: Compound 1 observed as a [M-H] - ion peak at m/z 353 in the ESI mass spectrum (fig. 2) and based on literature data[23] compound 1 was confirmed as a 5-O-caffeoyl quinic acid. By using Zorbax SB Phenyl column able to separate cis and trans isomer of 5-O-caffeoyl quinic acid (fig. 3) gave an [M-H]-ion peak at m/z 353 in the ESI mass spectrum (fig. 4). UV spectra also suggest that trans isomer is more absorption value compared to cis isomer (fig. 5). Mass spectral value of compound 2 showed a quasi-molecular ion [M-H] - at m/z 609, compared with literature data[24] compound 2 was identified as a quercetin-3-O-rutinoside (fig. 2).

Bottom Line: Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4).In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV.The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Sathyabama University, Chennai-600 119, India.

ABSTRACT
Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4). In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV. The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.

No MeSH data available.


Related in: MedlinePlus