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Matrix Solid-Phase Dispersion Extraction and Quantification of Alpinetin in Amomum Seed using Validated HPLC and HPTLC Methods.

Singh M, Kamal YT, Khan MA, Parveen R, Ansari SH, Ahmad S - Indian J Pharm Sci (2015 Jan-Feb)

Bottom Line: Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts.Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques.Validation data are indicative of good precision and accuracy and proved the reliability of the methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy and Phytochemistry, Hamdard University, Hamdard Nagar, New Delhi-110 062, India.

ABSTRACT
Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extraction. HPLC was performed on a C18 column with methanol and water (70:30, v/v) as mobile phase at a flow rate of 1.0 ml/min whereas HPTLC on silica aluminum sheet (60F254) using toluene, dichloromethane and ethyl acetate as solvent system. A sharp peak was obtained for alpinetin at a retention time (Rt) of 5.7 min by HPLC and retardation factor (Rf) of 0.48 by HPTLC. Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts. Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques. Validation data are indicative of good precision and accuracy and proved the reliability of the methods.

No MeSH data available.


Related in: MedlinePlus

Chemical structure of alpinetin.
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Figure 1: Chemical structure of alpinetin.

Mentions: Quality estimation by chemo profiling and marker constituent analysis using modern analytical methods is a tool for phytochemical evaluation. The WHO introduced chromatography for the quality assessment of the plant products and it is accepted as tools for identification and quality control of herbal medicines[12]. HPLC and HPTLC are widely accepted analytical techniques due to high accuracy, precision and reproducibility of results whereas, HPTLC has many advantages because of low operating cost and less time consuming[3]. The seeds of Amomum subulatum Roxb. (commonly known as greater cardamom) is a well known drug in Ayurvedic system of medicine in India[4]. The seeds mainly contain terpenes (cineole), flavonoids (alpinetin) and carbohydrates. The drug is aromatic pungent, stimulant, stomachic, alexipharmic, astringent and traditionally used for treatment of GIT disorders, rectal diseases, congestion of liver, pulmonary tuberculosis, as diuretic and as cardiac stimulant[5]. Orally administration of the drug was found to be useful in prevention of hyperlipidaemia and provide antioxidant protection[6]. It was evaluated in the treatment of facial skin wrinkles by prospective, open, Phase-III clinical trial and showed that the active constituents of A. subulatum have potent antioxidant activity[7]. The drug have significant ability to inhibit lipid peroxidation as well as it contains strong reducing power and superoxide radical scavenging activity due to their polyphenol content, protocatechualdehyde and protocatechuic acid[8910]. Alpinetin is a flavonoidal constituent (fig. 1), which is recently reported to posses vasorelaxant and as antiHIV activities[111213]. The beneficial properties of A. subulatum on heart disease were also observed and it was found that, one teaspoonful powder with honey twice a day was beneficial to patients with ischemic heart[14]. Protective effect of A. subulatum against stress[101516] also showed that the plant may have potential to cure various diseases related to free radical and metabolic oxidation.


Matrix Solid-Phase Dispersion Extraction and Quantification of Alpinetin in Amomum Seed using Validated HPLC and HPTLC Methods.

Singh M, Kamal YT, Khan MA, Parveen R, Ansari SH, Ahmad S - Indian J Pharm Sci (2015 Jan-Feb)

Chemical structure of alpinetin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355882&req=5

Figure 1: Chemical structure of alpinetin.
Mentions: Quality estimation by chemo profiling and marker constituent analysis using modern analytical methods is a tool for phytochemical evaluation. The WHO introduced chromatography for the quality assessment of the plant products and it is accepted as tools for identification and quality control of herbal medicines[12]. HPLC and HPTLC are widely accepted analytical techniques due to high accuracy, precision and reproducibility of results whereas, HPTLC has many advantages because of low operating cost and less time consuming[3]. The seeds of Amomum subulatum Roxb. (commonly known as greater cardamom) is a well known drug in Ayurvedic system of medicine in India[4]. The seeds mainly contain terpenes (cineole), flavonoids (alpinetin) and carbohydrates. The drug is aromatic pungent, stimulant, stomachic, alexipharmic, astringent and traditionally used for treatment of GIT disorders, rectal diseases, congestion of liver, pulmonary tuberculosis, as diuretic and as cardiac stimulant[5]. Orally administration of the drug was found to be useful in prevention of hyperlipidaemia and provide antioxidant protection[6]. It was evaluated in the treatment of facial skin wrinkles by prospective, open, Phase-III clinical trial and showed that the active constituents of A. subulatum have potent antioxidant activity[7]. The drug have significant ability to inhibit lipid peroxidation as well as it contains strong reducing power and superoxide radical scavenging activity due to their polyphenol content, protocatechualdehyde and protocatechuic acid[8910]. Alpinetin is a flavonoidal constituent (fig. 1), which is recently reported to posses vasorelaxant and as antiHIV activities[111213]. The beneficial properties of A. subulatum on heart disease were also observed and it was found that, one teaspoonful powder with honey twice a day was beneficial to patients with ischemic heart[14]. Protective effect of A. subulatum against stress[101516] also showed that the plant may have potential to cure various diseases related to free radical and metabolic oxidation.

Bottom Line: Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts.Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques.Validation data are indicative of good precision and accuracy and proved the reliability of the methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy and Phytochemistry, Hamdard University, Hamdard Nagar, New Delhi-110 062, India.

ABSTRACT
Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extraction. HPLC was performed on a C18 column with methanol and water (70:30, v/v) as mobile phase at a flow rate of 1.0 ml/min whereas HPTLC on silica aluminum sheet (60F254) using toluene, dichloromethane and ethyl acetate as solvent system. A sharp peak was obtained for alpinetin at a retention time (Rt) of 5.7 min by HPLC and retardation factor (Rf) of 0.48 by HPTLC. Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts. Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques. Validation data are indicative of good precision and accuracy and proved the reliability of the methods.

No MeSH data available.


Related in: MedlinePlus