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Determination of ethanol content in ayurvedic formulations kumaryasava and mustakarista by gas chromatography.

Selvan PS, Priya ES - Indian J Pharm Sci (2015 Jan-Feb)

Bottom Line: Evaluation of physiochemical properties and total phenolic contents resulted in establishing ranges for routine analysis.In both the formulations, ethanol content observed by specific gravity method varied from 0.9 to 1.9% on day 0 and reduced on storage for 30 days ranging from 0.1-0.6% v/v.This reduction in ethanol content on storage might be due to loss caused by frequent opening of the container.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Anna University BIT campus, Tiruchirappalli-620 024, India.

ABSTRACT
In the present study, two brands of Kumaryasava and Mustakarista medicinally important ayurvedic preparations were studied for quality control parameters like physiochemical properties, ethanol content by specific gravity and gas chromatographic methods and the total phenolic content. Evaluation of physiochemical properties and total phenolic contents resulted in establishing ranges for routine analysis. In both the formulations, ethanol content observed by specific gravity method varied from 0.9 to 1.9% on day 0 and reduced on storage for 30 days ranging from 0.1-0.6% v/v. Similarly, ethanol content determined by GC on day 0 varied from 0.1-1.6% v/v and on 30th day from 0.011-0.025% v/v. This reduction in ethanol content on storage might be due to loss caused by frequent opening of the container.

No MeSH data available.


Representative chromatogram of ethanol.
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Figure 1: Representative chromatogram of ethanol.

Mentions: Ethanol determination was carried out by two methods; specific gravity and gas chromatography and the results were tabulated in Table 3. In both the formulations, the ethanol content observed by specific gravity method varied from 0.9 to 1.9% on day 0 and reduced on storage for 30 days to 0.1-0.6% v/v. The same way followed in the ethanol determination by gas chromatographic method, where the ethanol content was decreased after a certain period of time (0, 30 days). Representative chromatograms are shown for standard ethanol (fig. 1), ethanol in B (Kumaryasava) at 0th and 30th day (fig. 2), ethanol in F (Mustakarista) at 0th and 30th day (fig. 3). The ethanol content determined on day 0 varied from 0.1-1.6% v/v and on 30th day it reduced to 0.011-0.025% v/v. There is marked difference in ethanol content determined by specific gravity and GC method, which may be considered as the difference between the conventional and the modern method. GC method may provide much accurate and precise result when compared to specific gravity method. A similar variation in alcohol content of Kankasava preparation was also observed by two different methods[11]. In both the methods the ethanol content has shown marked reduction on storage of the formulations. The ethanol content of the formulations was found to be under the limit specified by WHO as 5-10%. Variation in alcohol content of Mustakarista was observed with different containers used in manufacturing process[12]. Thus the container used in the manufacturing process might also influence the alcohol content in the formulations. The reduction in the ethanol content observed from the results on day 30 of the experiment shows that the self-generated alcohol in the formulation might be vaporized on opening the container. Thus the asava and arista preparations have to be consumed in a shorter period of time or else the formulations can be made in small volume containers for divided dosages.


Determination of ethanol content in ayurvedic formulations kumaryasava and mustakarista by gas chromatography.

Selvan PS, Priya ES - Indian J Pharm Sci (2015 Jan-Feb)

Representative chromatogram of ethanol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355876&req=5

Figure 1: Representative chromatogram of ethanol.
Mentions: Ethanol determination was carried out by two methods; specific gravity and gas chromatography and the results were tabulated in Table 3. In both the formulations, the ethanol content observed by specific gravity method varied from 0.9 to 1.9% on day 0 and reduced on storage for 30 days to 0.1-0.6% v/v. The same way followed in the ethanol determination by gas chromatographic method, where the ethanol content was decreased after a certain period of time (0, 30 days). Representative chromatograms are shown for standard ethanol (fig. 1), ethanol in B (Kumaryasava) at 0th and 30th day (fig. 2), ethanol in F (Mustakarista) at 0th and 30th day (fig. 3). The ethanol content determined on day 0 varied from 0.1-1.6% v/v and on 30th day it reduced to 0.011-0.025% v/v. There is marked difference in ethanol content determined by specific gravity and GC method, which may be considered as the difference between the conventional and the modern method. GC method may provide much accurate and precise result when compared to specific gravity method. A similar variation in alcohol content of Kankasava preparation was also observed by two different methods[11]. In both the methods the ethanol content has shown marked reduction on storage of the formulations. The ethanol content of the formulations was found to be under the limit specified by WHO as 5-10%. Variation in alcohol content of Mustakarista was observed with different containers used in manufacturing process[12]. Thus the container used in the manufacturing process might also influence the alcohol content in the formulations. The reduction in the ethanol content observed from the results on day 30 of the experiment shows that the self-generated alcohol in the formulation might be vaporized on opening the container. Thus the asava and arista preparations have to be consumed in a shorter period of time or else the formulations can be made in small volume containers for divided dosages.

Bottom Line: Evaluation of physiochemical properties and total phenolic contents resulted in establishing ranges for routine analysis.In both the formulations, ethanol content observed by specific gravity method varied from 0.9 to 1.9% on day 0 and reduced on storage for 30 days ranging from 0.1-0.6% v/v.This reduction in ethanol content on storage might be due to loss caused by frequent opening of the container.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Anna University BIT campus, Tiruchirappalli-620 024, India.

ABSTRACT
In the present study, two brands of Kumaryasava and Mustakarista medicinally important ayurvedic preparations were studied for quality control parameters like physiochemical properties, ethanol content by specific gravity and gas chromatographic methods and the total phenolic content. Evaluation of physiochemical properties and total phenolic contents resulted in establishing ranges for routine analysis. In both the formulations, ethanol content observed by specific gravity method varied from 0.9 to 1.9% on day 0 and reduced on storage for 30 days ranging from 0.1-0.6% v/v. Similarly, ethanol content determined by GC on day 0 varied from 0.1-1.6% v/v and on 30th day from 0.011-0.025% v/v. This reduction in ethanol content on storage might be due to loss caused by frequent opening of the container.

No MeSH data available.