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TLC and HPTLC Fingerprints of Various Secondary Metabolites in the Stem of the Traditional Medicinal Climber, Solena amplexicaulis.

Karthika K, Paulsamy S - Indian J Pharm Sci (2015 Jan-Feb)

Bottom Line: These studies were carried out as per the methods of Harborne and Wagner et al.The methanol extract of the stem showed the presence of 6 alkaloids, 6 flavonoids, 2 glycosides, 9 saponins and 3 terpenoids.Owing to the presence of rich variety of secondary metabolites, the stem extract of S. amplexicaulis is expected to exhibit therapeutic properties.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Botany, Kongunadu Arts and Science College, Coimbatore - 641 029, India.

ABSTRACT
Aim of this study was to develop a TLC and a HPTLC fingerprint profiles for various secondary metabolites of methanol extracts of the stem of the traditional medicinal climber, Solena amplexicaulis. These studies were carried out as per the methods of Harborne and Wagner et al. The profiles of various individual secondary metabolites were made and developed for authentication. The methanol extract of the stem showed the presence of 6 alkaloids, 6 flavonoids, 2 glycosides, 9 saponins and 3 terpenoids. Owing to the presence of rich variety of secondary metabolites, the stem extract of S. amplexicaulis is expected to exhibit therapeutic properties.

No MeSH data available.


Related in: MedlinePlus

HPTLC fingerprinting profile and densitogram of methanol extract of the stem of Solena amplexicaulis.Fingerprinting profile and densitograms of different phytochemicals such as (a) alkaloids, (b) flavonoids, (c) glycosides, (d) saponins and (e) terpenoids separated from the methanol extract of the stems of Solena amplexicaulis using HPTLC.
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Figure 1: HPTLC fingerprinting profile and densitogram of methanol extract of the stem of Solena amplexicaulis.Fingerprinting profile and densitograms of different phytochemicals such as (a) alkaloids, (b) flavonoids, (c) glycosides, (d) saponins and (e) terpenoids separated from the methanol extract of the stems of Solena amplexicaulis using HPTLC.

Mentions: HPTLC alkaloid profile of methanol extract of the S. amplexicaulis stem was presented in Tables 2 and 3 and fig. 1a. Bright orange colour zone at visible light mode was observed in the chromatogram after derivatization, which confirmed the presence of alkaloids in the sample as in the standard. Thirteen compounds were separated and of which 6 were alkaloids at the Rf in the range of 0.18-0.59. The highest peak area was 11283.1 AU and that of the lower one was 1424.5 AU, which were observed at Rf of 0.40 and 0.59, respectively. It is not uncommon to find alkaloids since more than 10 000 different alkaloids have been identified in species from over 300 plant families[19].


TLC and HPTLC Fingerprints of Various Secondary Metabolites in the Stem of the Traditional Medicinal Climber, Solena amplexicaulis.

Karthika K, Paulsamy S - Indian J Pharm Sci (2015 Jan-Feb)

HPTLC fingerprinting profile and densitogram of methanol extract of the stem of Solena amplexicaulis.Fingerprinting profile and densitograms of different phytochemicals such as (a) alkaloids, (b) flavonoids, (c) glycosides, (d) saponins and (e) terpenoids separated from the methanol extract of the stems of Solena amplexicaulis using HPTLC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355874&req=5

Figure 1: HPTLC fingerprinting profile and densitogram of methanol extract of the stem of Solena amplexicaulis.Fingerprinting profile and densitograms of different phytochemicals such as (a) alkaloids, (b) flavonoids, (c) glycosides, (d) saponins and (e) terpenoids separated from the methanol extract of the stems of Solena amplexicaulis using HPTLC.
Mentions: HPTLC alkaloid profile of methanol extract of the S. amplexicaulis stem was presented in Tables 2 and 3 and fig. 1a. Bright orange colour zone at visible light mode was observed in the chromatogram after derivatization, which confirmed the presence of alkaloids in the sample as in the standard. Thirteen compounds were separated and of which 6 were alkaloids at the Rf in the range of 0.18-0.59. The highest peak area was 11283.1 AU and that of the lower one was 1424.5 AU, which were observed at Rf of 0.40 and 0.59, respectively. It is not uncommon to find alkaloids since more than 10 000 different alkaloids have been identified in species from over 300 plant families[19].

Bottom Line: These studies were carried out as per the methods of Harborne and Wagner et al.The methanol extract of the stem showed the presence of 6 alkaloids, 6 flavonoids, 2 glycosides, 9 saponins and 3 terpenoids.Owing to the presence of rich variety of secondary metabolites, the stem extract of S. amplexicaulis is expected to exhibit therapeutic properties.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Botany, Kongunadu Arts and Science College, Coimbatore - 641 029, India.

ABSTRACT
Aim of this study was to develop a TLC and a HPTLC fingerprint profiles for various secondary metabolites of methanol extracts of the stem of the traditional medicinal climber, Solena amplexicaulis. These studies were carried out as per the methods of Harborne and Wagner et al. The profiles of various individual secondary metabolites were made and developed for authentication. The methanol extract of the stem showed the presence of 6 alkaloids, 6 flavonoids, 2 glycosides, 9 saponins and 3 terpenoids. Owing to the presence of rich variety of secondary metabolites, the stem extract of S. amplexicaulis is expected to exhibit therapeutic properties.

No MeSH data available.


Related in: MedlinePlus