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Preparation and characterization of (125)i labeled bovine serum albumin.

Ashwitha Rai KS - Indian J Pharm Sci (2015 Jan-Feb)

Bottom Line: Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine.Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity.Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity.

View Article: PubMed Central - PubMed

Affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.

ABSTRACT
Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity.

No MeSH data available.


Optimization of incubation time.Optimization of incubation time with the duration of the reaction on X-axis against percentage yield on the Y-axis.
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Figure 2: Optimization of incubation time.Optimization of incubation time with the duration of the reaction on X-axis against percentage yield on the Y-axis.

Mentions: The concentration of chloramine-T and MBS of 10 and 20 μg chosen for the studies were further optimized with different incubation timings as shown in the fig. 2. The maximum yield was obtained at 60 seconds (71%). When the incubation timings were increased further the yield reduced which may be due to the damage caused to the BSA protein during labeling. Effect of temperature was also studied on the radioiodination yield i.e. at 28° and 2° and it was seen that the reaction when performed at lower temperature (2°) gave yield of 75% (fig. 3).


Preparation and characterization of (125)i labeled bovine serum albumin.

Ashwitha Rai KS - Indian J Pharm Sci (2015 Jan-Feb)

Optimization of incubation time.Optimization of incubation time with the duration of the reaction on X-axis against percentage yield on the Y-axis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355873&req=5

Figure 2: Optimization of incubation time.Optimization of incubation time with the duration of the reaction on X-axis against percentage yield on the Y-axis.
Mentions: The concentration of chloramine-T and MBS of 10 and 20 μg chosen for the studies were further optimized with different incubation timings as shown in the fig. 2. The maximum yield was obtained at 60 seconds (71%). When the incubation timings were increased further the yield reduced which may be due to the damage caused to the BSA protein during labeling. Effect of temperature was also studied on the radioiodination yield i.e. at 28° and 2° and it was seen that the reaction when performed at lower temperature (2°) gave yield of 75% (fig. 3).

Bottom Line: Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine.Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity.Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity.

View Article: PubMed Central - PubMed

Affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.

ABSTRACT
Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity.

No MeSH data available.