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Gastric mucosal protection by aegle marmelos against gastric mucosal damage: role of enterochromaffin cell and serotonin.

Singh P, Dutta SR, Guha D - Saudi J Gastroenterol (2015 Jan-Feb)

Bottom Line: Ulceration was induced by using aspirin (500 mg/kg, p.o.), cerebellar nodular lesion and applying cold-restraint stress.In all cases increased MUI in gastric tissue along with decreased EC cell count was observed with concomitant decrease of 5-HT content and adherent mucosal thickness (P < 0.05).AM produces gastric mucosal protection mediated by increased EC cell count and 5-HT levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Vyas Dental College and Hospital, Jodhpur, Rajasthan, India.

ABSTRACT

Background/aims: Serotonin (5-hydroxytryptamine; 5-HT) released from enterochromaffin (EC) cells in gastric mucosa inhibits gastric acidity by increasing the gastric mucus secretion. In the present study, we evaluated the effect of aqueous extract of Aegle marmelos (AM) ripe fruit pulp (250 mg/kg body weight) on mean ulcer index (MUI), EC cells, 5-HT content, and adherent mucosal thickness of ulcerated gastric tissue in adult albino rats.

Material and methods: Ulceration was induced by using aspirin (500 mg/kg, p.o.), cerebellar nodular lesion and applying cold-restraint stress.

Results: In all cases increased MUI in gastric tissue along with decreased EC cell count was observed with concomitant decrease of 5-HT content and adherent mucosal thickness (P < 0.05). Pretreatment with AM for 14 days decreased MUI, increased EC cell count, and 5-HT content as well as adherent mucosal thickness in all ulcerated group (P < 0.05).

Conclusion: AM produces gastric mucosal protection mediated by increased EC cell count and 5-HT levels.

No MeSH data available.


Related in: MedlinePlus

Representative histomorphological picture depicting the changes in the gastric mucosal layer in ulcerated condition induced by aspirin (ASP), cerebellar lesion (CNL), and cold-restrain stress (CRS), and the protection rendered by Aegle marmelos (AM) pretreatment (arrows show the damage to the mucosal lining). (a) presents the control group, (b) represents the only Aegle marmelos-treated group (AM), (c) represents the aspirin-induced ulcerated group (ASP), (d) represents the Aegle marmelos-pretreated aspirin-induced ulcerated group (AM+ASP), (e) represents the cerebellar-lesion-induced ulcerated group (CNL), (f) represents the Aegle marmelos-pretreated cerebellar-lesion-induced ulcerated group (AM+CNL), (g) represents the cold-restraint stress-induced ulcerated group (CRS), and (h) represents the Aegle marmelos-pretreated cold-restraint stress-induced ulcerated group (AM+CRS)
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Figure 1: Representative histomorphological picture depicting the changes in the gastric mucosal layer in ulcerated condition induced by aspirin (ASP), cerebellar lesion (CNL), and cold-restrain stress (CRS), and the protection rendered by Aegle marmelos (AM) pretreatment (arrows show the damage to the mucosal lining). (a) presents the control group, (b) represents the only Aegle marmelos-treated group (AM), (c) represents the aspirin-induced ulcerated group (ASP), (d) represents the Aegle marmelos-pretreated aspirin-induced ulcerated group (AM+ASP), (e) represents the cerebellar-lesion-induced ulcerated group (CNL), (f) represents the Aegle marmelos-pretreated cerebellar-lesion-induced ulcerated group (AM+CNL), (g) represents the cold-restraint stress-induced ulcerated group (CRS), and (h) represents the Aegle marmelos-pretreated cold-restraint stress-induced ulcerated group (AM+CRS)

Mentions: Figure 1a and 1b shows the gastric mucosa of control and AM only treated rat, respectively, as observed under the stereomicroscope with bright illumination. In all preparations, the surface adherent gel layer was optically distinct from the dense mucosa. The mucus gel layer is easily recognized as a light-pink stained material covering the surface epithelium. All investigated areas of mucosa were covered by mucus. There is disruption of mucus lining following treatment with aspirin [Figure 1c], but the mucus lining appeared continuous on pretreatment with AM [Figure 1d]. There is severe loss of surface mucus lining following cerebellar lesion [Figure 1e]. Pretreatment with AM leads to production of huge amount of mucus, thereby preventing the damage to gastric cells and maintaining the continuity of mucus [Figure 1f]. In Figure 1g, it can be seen that chronic stress due to exposure of rats to cold-restraint condition leads to production of severe erosions marked by the presence of heterogeneous mixture of tissues retracting from the mucosal surface. A variety of surface epithelial changes in shape, size, and orientation, accompanied by marked loss of surface mucus epithelial cell were found. Marked disorganization and atrophy of glands were invariably noted. This seems to be prevented by AM pretreatment, which maintains the adherent mucus lining resisting the erosion of glandular cells as seen in Figure 1h.


Gastric mucosal protection by aegle marmelos against gastric mucosal damage: role of enterochromaffin cell and serotonin.

Singh P, Dutta SR, Guha D - Saudi J Gastroenterol (2015 Jan-Feb)

Representative histomorphological picture depicting the changes in the gastric mucosal layer in ulcerated condition induced by aspirin (ASP), cerebellar lesion (CNL), and cold-restrain stress (CRS), and the protection rendered by Aegle marmelos (AM) pretreatment (arrows show the damage to the mucosal lining). (a) presents the control group, (b) represents the only Aegle marmelos-treated group (AM), (c) represents the aspirin-induced ulcerated group (ASP), (d) represents the Aegle marmelos-pretreated aspirin-induced ulcerated group (AM+ASP), (e) represents the cerebellar-lesion-induced ulcerated group (CNL), (f) represents the Aegle marmelos-pretreated cerebellar-lesion-induced ulcerated group (AM+CNL), (g) represents the cold-restraint stress-induced ulcerated group (CRS), and (h) represents the Aegle marmelos-pretreated cold-restraint stress-induced ulcerated group (AM+CRS)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355861&req=5

Figure 1: Representative histomorphological picture depicting the changes in the gastric mucosal layer in ulcerated condition induced by aspirin (ASP), cerebellar lesion (CNL), and cold-restrain stress (CRS), and the protection rendered by Aegle marmelos (AM) pretreatment (arrows show the damage to the mucosal lining). (a) presents the control group, (b) represents the only Aegle marmelos-treated group (AM), (c) represents the aspirin-induced ulcerated group (ASP), (d) represents the Aegle marmelos-pretreated aspirin-induced ulcerated group (AM+ASP), (e) represents the cerebellar-lesion-induced ulcerated group (CNL), (f) represents the Aegle marmelos-pretreated cerebellar-lesion-induced ulcerated group (AM+CNL), (g) represents the cold-restraint stress-induced ulcerated group (CRS), and (h) represents the Aegle marmelos-pretreated cold-restraint stress-induced ulcerated group (AM+CRS)
Mentions: Figure 1a and 1b shows the gastric mucosa of control and AM only treated rat, respectively, as observed under the stereomicroscope with bright illumination. In all preparations, the surface adherent gel layer was optically distinct from the dense mucosa. The mucus gel layer is easily recognized as a light-pink stained material covering the surface epithelium. All investigated areas of mucosa were covered by mucus. There is disruption of mucus lining following treatment with aspirin [Figure 1c], but the mucus lining appeared continuous on pretreatment with AM [Figure 1d]. There is severe loss of surface mucus lining following cerebellar lesion [Figure 1e]. Pretreatment with AM leads to production of huge amount of mucus, thereby preventing the damage to gastric cells and maintaining the continuity of mucus [Figure 1f]. In Figure 1g, it can be seen that chronic stress due to exposure of rats to cold-restraint condition leads to production of severe erosions marked by the presence of heterogeneous mixture of tissues retracting from the mucosal surface. A variety of surface epithelial changes in shape, size, and orientation, accompanied by marked loss of surface mucus epithelial cell were found. Marked disorganization and atrophy of glands were invariably noted. This seems to be prevented by AM pretreatment, which maintains the adherent mucus lining resisting the erosion of glandular cells as seen in Figure 1h.

Bottom Line: Ulceration was induced by using aspirin (500 mg/kg, p.o.), cerebellar nodular lesion and applying cold-restraint stress.In all cases increased MUI in gastric tissue along with decreased EC cell count was observed with concomitant decrease of 5-HT content and adherent mucosal thickness (P < 0.05).AM produces gastric mucosal protection mediated by increased EC cell count and 5-HT levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Vyas Dental College and Hospital, Jodhpur, Rajasthan, India.

ABSTRACT

Background/aims: Serotonin (5-hydroxytryptamine; 5-HT) released from enterochromaffin (EC) cells in gastric mucosa inhibits gastric acidity by increasing the gastric mucus secretion. In the present study, we evaluated the effect of aqueous extract of Aegle marmelos (AM) ripe fruit pulp (250 mg/kg body weight) on mean ulcer index (MUI), EC cells, 5-HT content, and adherent mucosal thickness of ulcerated gastric tissue in adult albino rats.

Material and methods: Ulceration was induced by using aspirin (500 mg/kg, p.o.), cerebellar nodular lesion and applying cold-restraint stress.

Results: In all cases increased MUI in gastric tissue along with decreased EC cell count was observed with concomitant decrease of 5-HT content and adherent mucosal thickness (P < 0.05). Pretreatment with AM for 14 days decreased MUI, increased EC cell count, and 5-HT content as well as adherent mucosal thickness in all ulcerated group (P < 0.05).

Conclusion: AM produces gastric mucosal protection mediated by increased EC cell count and 5-HT levels.

No MeSH data available.


Related in: MedlinePlus