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Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.

Bharath MJ, Sahadev CK, Ramachandra PK, Rudranaik S, George J, Thomas A - J Int Soc Prev Community Dent (2015 Jan-Feb)

Bottom Line: Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.

ABSTRACT

Objectives: The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.

Materials and methods: Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.

Results: The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.

Conclusion: Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

No MeSH data available.


Related in: MedlinePlus

Optical calorimeter
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Figure 5: Optical calorimeter

Mentions: To standardize the number of cells, a cell suspension was made to get a cell count of 40,000 cells/ml. The cells were counted with a Neubauer counting chamber under light microscope. One milliliter of each of the storage media was inoculated into the 96-well culture plates containing the fibroblasts cells and placed in an incubator of 5% CO2 at 37°C for 24 h. Then 50 μl of MTT dye was added to each well and the contents were incubated for 4 h at 37°C. After incubation, the MTT dye was removed and dimethyl sulfoxide (DMSO) solvent was added to solubilize the colored products. The viable fibroblast cells absorbed the dye and a purplish residue containing formazan crystals was formed in most of the wells. The absorbance was measured using an optical microplate reader or called as optical calorimeter [Figure 5] at a wavelength of 540 nm and the percentage of viable cells was calculated for 15 min, 30 min, 60 min, and 90 min. A graph of concentration versus inhibition was plotted.


Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.

Bharath MJ, Sahadev CK, Ramachandra PK, Rudranaik S, George J, Thomas A - J Int Soc Prev Community Dent (2015 Jan-Feb)

Optical calorimeter
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355854&req=5

Figure 5: Optical calorimeter
Mentions: To standardize the number of cells, a cell suspension was made to get a cell count of 40,000 cells/ml. The cells were counted with a Neubauer counting chamber under light microscope. One milliliter of each of the storage media was inoculated into the 96-well culture plates containing the fibroblasts cells and placed in an incubator of 5% CO2 at 37°C for 24 h. Then 50 μl of MTT dye was added to each well and the contents were incubated for 4 h at 37°C. After incubation, the MTT dye was removed and dimethyl sulfoxide (DMSO) solvent was added to solubilize the colored products. The viable fibroblast cells absorbed the dye and a purplish residue containing formazan crystals was formed in most of the wells. The absorbance was measured using an optical microplate reader or called as optical calorimeter [Figure 5] at a wavelength of 540 nm and the percentage of viable cells was calculated for 15 min, 30 min, 60 min, and 90 min. A graph of concentration versus inhibition was plotted.

Bottom Line: Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.

ABSTRACT

Objectives: The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.

Materials and methods: Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.

Results: The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.

Conclusion: Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

No MeSH data available.


Related in: MedlinePlus