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Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.

Bharath MJ, Sahadev CK, Ramachandra PK, Rudranaik S, George J, Thomas A - J Int Soc Prev Community Dent (2015 Jan-Feb)

Bottom Line: Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.

ABSTRACT

Objectives: The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.

Materials and methods: Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.

Results: The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.

Conclusion: Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

No MeSH data available.


Related in: MedlinePlus

The four storage medias used in the present study
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Figure 4: The four storage medias used in the present study

Mentions: The storage media tested in the study were divided into four major groups to be inoculated into the 96-well culture plates as follows [Figure 4]:


Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.

Bharath MJ, Sahadev CK, Ramachandra PK, Rudranaik S, George J, Thomas A - J Int Soc Prev Community Dent (2015 Jan-Feb)

The four storage medias used in the present study
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355854&req=5

Figure 4: The four storage medias used in the present study
Mentions: The storage media tested in the study were divided into four major groups to be inoculated into the 96-well culture plates as follows [Figure 4]:

Bottom Line: Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.

ABSTRACT

Objectives: The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.

Materials and methods: Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.

Results: The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.

Conclusion: Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

No MeSH data available.


Related in: MedlinePlus