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Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.

Bharath MJ, Sahadev CK, Ramachandra PK, Rudranaik S, George J, Thomas A - J Int Soc Prev Community Dent (2015 Jan-Feb)

Bottom Line: Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.

ABSTRACT

Objectives: The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.

Materials and methods: Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.

Results: The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.

Conclusion: Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

No MeSH data available.


Related in: MedlinePlus

Microlittre pipette
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Figure 3: Microlittre pipette

Mentions: The 96-well culture plates were incubated at 37°C for 24 h with a layer of fibroblasts attached to the well culture plates and they were seeded at a density of 40,000 cells/well onto the 96-well culture plates using a microlittre pipette [Figures 2 and 3].


Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.

Bharath MJ, Sahadev CK, Ramachandra PK, Rudranaik S, George J, Thomas A - J Int Soc Prev Community Dent (2015 Jan-Feb)

Microlittre pipette
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355854&req=5

Figure 3: Microlittre pipette
Mentions: The 96-well culture plates were incubated at 37°C for 24 h with a layer of fibroblasts attached to the well culture plates and they were seeded at a density of 40,000 cells/well onto the 96-well culture plates using a microlittre pipette [Figures 2 and 3].

Bottom Line: Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.

ABSTRACT

Objectives: The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.

Materials and methods: Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.

Results: The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.

Conclusion: Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

No MeSH data available.


Related in: MedlinePlus