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Gingival crevicular fluid: As a diagnostic marker in HIV positive patients.

Atram P, Patil P, Saify F, Rathod V, Gotmare S - J Int Soc Prev Community Dent (2015 Jan-Feb)

Bottom Line: After the clinical observations the data were collected and tabulated for statistical analysis.When compared with serum, the sensitivity, specificity, and positive and negative predictive values of GCF were 100% respectively.All the above findings are suggestive of GCF being a better diagnostic medium than saliva.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, RCDSR, Bhilai, Chhattisgarh, India.

ABSTRACT

Objective: To determine the utility of gingival crevicular fluid (GCF) for the detection of anti-HIV antibodies in human immunodeficiency virus (HIV)-infected individuals using enzyme linked immunosorbent assay (ELISA) with respect to their CD4 counts.

Materials and methods: The study was carried out between 37 seropositive (37GCF, 10 saliva) and 37 seronegative (GCF) individuals for a period of 7-8 months in GDC Nagpur. Thirty-seven GCF and 10 whole saliva samples were collected from the same patient. GCF samples were collected from gingival crevice with the help of Kimble disposable microcapillary. Saliva was collected by asking the patient to bend forward. The drooling saliva was collected in a sterile bottle and stored at Minus 20°C (-20°C). After the clinical observations the data were collected and tabulated for statistical analysis.

Results: When compared with serum, the sensitivity, specificity, and positive and negative predictive values of GCF were 100% respectively.

Conclusion: All the above findings are suggestive of GCF being a better diagnostic medium than saliva.

No MeSH data available.


Related in: MedlinePlus

HIV - Kit
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Figure 2: HIV - Kit

Mentions: By using HIV Chex ELISA kit GCF samples were processed according to the manufacturers instructions. Figures 2 and 3. The samples were brought to room temperatur e. 96 well dilution plate was taken and 250 μl of diluents was pipette into the well. Then 5 μl GCF was added to each well with the help of expirator and mix. Disposable tips were used for each sample. 100 μl of prediluted sample was transferred to the wells and incubated at room temperature for 30 min with cover plate. Wash for five cycles. Add 100 μl of working conjugate to the well, incubate it for 30 min at room temperature with the lid covered. Wash for another 5 cycles. 100 μl of chromogenic substrate was added the well and incubated in dark for 10 min at room temperature. Lastly add 50 μl of stop solution and read under ELISA reader at 450 nm/650 nm within one hour Figures 4 and 5.


Gingival crevicular fluid: As a diagnostic marker in HIV positive patients.

Atram P, Patil P, Saify F, Rathod V, Gotmare S - J Int Soc Prev Community Dent (2015 Jan-Feb)

HIV - Kit
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355846&req=5

Figure 2: HIV - Kit
Mentions: By using HIV Chex ELISA kit GCF samples were processed according to the manufacturers instructions. Figures 2 and 3. The samples were brought to room temperatur e. 96 well dilution plate was taken and 250 μl of diluents was pipette into the well. Then 5 μl GCF was added to each well with the help of expirator and mix. Disposable tips were used for each sample. 100 μl of prediluted sample was transferred to the wells and incubated at room temperature for 30 min with cover plate. Wash for five cycles. Add 100 μl of working conjugate to the well, incubate it for 30 min at room temperature with the lid covered. Wash for another 5 cycles. 100 μl of chromogenic substrate was added the well and incubated in dark for 10 min at room temperature. Lastly add 50 μl of stop solution and read under ELISA reader at 450 nm/650 nm within one hour Figures 4 and 5.

Bottom Line: After the clinical observations the data were collected and tabulated for statistical analysis.When compared with serum, the sensitivity, specificity, and positive and negative predictive values of GCF were 100% respectively.All the above findings are suggestive of GCF being a better diagnostic medium than saliva.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, RCDSR, Bhilai, Chhattisgarh, India.

ABSTRACT

Objective: To determine the utility of gingival crevicular fluid (GCF) for the detection of anti-HIV antibodies in human immunodeficiency virus (HIV)-infected individuals using enzyme linked immunosorbent assay (ELISA) with respect to their CD4 counts.

Materials and methods: The study was carried out between 37 seropositive (37GCF, 10 saliva) and 37 seronegative (GCF) individuals for a period of 7-8 months in GDC Nagpur. Thirty-seven GCF and 10 whole saliva samples were collected from the same patient. GCF samples were collected from gingival crevice with the help of Kimble disposable microcapillary. Saliva was collected by asking the patient to bend forward. The drooling saliva was collected in a sterile bottle and stored at Minus 20°C (-20°C). After the clinical observations the data were collected and tabulated for statistical analysis.

Results: When compared with serum, the sensitivity, specificity, and positive and negative predictive values of GCF were 100% respectively.

Conclusion: All the above findings are suggestive of GCF being a better diagnostic medium than saliva.

No MeSH data available.


Related in: MedlinePlus