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Evaluation of a topical herbal agent for the promotion of bone healing.

Siu WS, Ko CH, Lam KW, Wat E, Shum WT, Lau CB, Ko KM, Hung LK, Lau DT, Leung PC - Evid Based Complement Alternat Med (2015)

Bottom Line: This indicated its anti-inflammatory effect.However, CDNR did not affect the bone turnover markers in serum of the rats.With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, 5/F, The CUHK Hong Kong Jockey Club School of Public Health Building, Prince of Wales Hospital, Shatin, New Territories, Hong Kong ; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong ; Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
A topically used Chinese herbal paste, namely, CDNR, was designed to facilitate fracture healing which is usually not addressed in general hospital care. From our in vitro studies, CDNR significantly inhibited the release of nitric oxide from RAW264.7 cells by 51 to 77%. This indicated its anti-inflammatory effect. CDNR also promoted the growth of bone cells by stimulating the proliferation of UMR106 cells up to 18%. It also increased the biomechanical strength of the healing bone in a drill-hole defect rat model by 16.5% significantly. This result revealed its in vivo efficacy on facilitation of bone healing. Furthermore, the detection of the chemical markers of CDNR in the skin and muscle of the treatment area demonstrated its transdermal properties. However, CDNR did not affect the bone turnover markers in serum of the rats. With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

No MeSH data available.


Related in: MedlinePlus

Concentration of the chemical markers of CDNR remained in the soft tissues of the rat. (a) Skin; (b) muscle. Data are expressed as mean and standard deviation (error bar). Rg1: ginsenoside Rg1; Rb1: ginsenoside Rb1; ASP: asperosaponin VI; OA: oleanolic acid; EMO: emodin; RHE: rhein; HYA: hydroxysafflor yellow A; KAE: kaempferol.
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fig4: Concentration of the chemical markers of CDNR remained in the soft tissues of the rat. (a) Skin; (b) muscle. Data are expressed as mean and standard deviation (error bar). Rg1: ginsenoside Rg1; Rb1: ginsenoside Rb1; ASP: asperosaponin VI; OA: oleanolic acid; EMO: emodin; RHE: rhein; HYA: hydroxysafflor yellow A; KAE: kaempferol.

Mentions: The transdermal transport of CDNR was determined by identifying the relevant chemical marker compounds in the skin and muscle after 6 weeks of herbal treatment using Q-TOF LC/MS technique. As shown in Figure 4(a), all the chemical markers of CDNR, except oleanolic acid (OA), were detected in the rat skin with asperosaponin VI (ASP) showing the most abundance. Similarly, those 7 chemical markers detected in the skin, except kaempferol (KAE), could also be found in the muscle. OA was nondetectable neither in the muscle. With a little bit difference from skin, rhein (RHE) but not the ASP was found to be the most abundant in the muscle (Figure 4(b)).


Evaluation of a topical herbal agent for the promotion of bone healing.

Siu WS, Ko CH, Lam KW, Wat E, Shum WT, Lau CB, Ko KM, Hung LK, Lau DT, Leung PC - Evid Based Complement Alternat Med (2015)

Concentration of the chemical markers of CDNR remained in the soft tissues of the rat. (a) Skin; (b) muscle. Data are expressed as mean and standard deviation (error bar). Rg1: ginsenoside Rg1; Rb1: ginsenoside Rb1; ASP: asperosaponin VI; OA: oleanolic acid; EMO: emodin; RHE: rhein; HYA: hydroxysafflor yellow A; KAE: kaempferol.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4355818&req=5

fig4: Concentration of the chemical markers of CDNR remained in the soft tissues of the rat. (a) Skin; (b) muscle. Data are expressed as mean and standard deviation (error bar). Rg1: ginsenoside Rg1; Rb1: ginsenoside Rb1; ASP: asperosaponin VI; OA: oleanolic acid; EMO: emodin; RHE: rhein; HYA: hydroxysafflor yellow A; KAE: kaempferol.
Mentions: The transdermal transport of CDNR was determined by identifying the relevant chemical marker compounds in the skin and muscle after 6 weeks of herbal treatment using Q-TOF LC/MS technique. As shown in Figure 4(a), all the chemical markers of CDNR, except oleanolic acid (OA), were detected in the rat skin with asperosaponin VI (ASP) showing the most abundance. Similarly, those 7 chemical markers detected in the skin, except kaempferol (KAE), could also be found in the muscle. OA was nondetectable neither in the muscle. With a little bit difference from skin, rhein (RHE) but not the ASP was found to be the most abundant in the muscle (Figure 4(b)).

Bottom Line: This indicated its anti-inflammatory effect.However, CDNR did not affect the bone turnover markers in serum of the rats.With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, 5/F, The CUHK Hong Kong Jockey Club School of Public Health Building, Prince of Wales Hospital, Shatin, New Territories, Hong Kong ; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong ; Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
A topically used Chinese herbal paste, namely, CDNR, was designed to facilitate fracture healing which is usually not addressed in general hospital care. From our in vitro studies, CDNR significantly inhibited the release of nitric oxide from RAW264.7 cells by 51 to 77%. This indicated its anti-inflammatory effect. CDNR also promoted the growth of bone cells by stimulating the proliferation of UMR106 cells up to 18%. It also increased the biomechanical strength of the healing bone in a drill-hole defect rat model by 16.5% significantly. This result revealed its in vivo efficacy on facilitation of bone healing. Furthermore, the detection of the chemical markers of CDNR in the skin and muscle of the treatment area demonstrated its transdermal properties. However, CDNR did not affect the bone turnover markers in serum of the rats. With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

No MeSH data available.


Related in: MedlinePlus