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Evaluation of a topical herbal agent for the promotion of bone healing.

Siu WS, Ko CH, Lam KW, Wat E, Shum WT, Lau CB, Ko KM, Hung LK, Lau DT, Leung PC - Evid Based Complement Alternat Med (2015)

Bottom Line: This indicated its anti-inflammatory effect.However, CDNR did not affect the bone turnover markers in serum of the rats.With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, 5/F, The CUHK Hong Kong Jockey Club School of Public Health Building, Prince of Wales Hospital, Shatin, New Territories, Hong Kong ; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong ; Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
A topically used Chinese herbal paste, namely, CDNR, was designed to facilitate fracture healing which is usually not addressed in general hospital care. From our in vitro studies, CDNR significantly inhibited the release of nitric oxide from RAW264.7 cells by 51 to 77%. This indicated its anti-inflammatory effect. CDNR also promoted the growth of bone cells by stimulating the proliferation of UMR106 cells up to 18%. It also increased the biomechanical strength of the healing bone in a drill-hole defect rat model by 16.5% significantly. This result revealed its in vivo efficacy on facilitation of bone healing. Furthermore, the detection of the chemical markers of CDNR in the skin and muscle of the treatment area demonstrated its transdermal properties. However, CDNR did not affect the bone turnover markers in serum of the rats. With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

No MeSH data available.


Related in: MedlinePlus

Osteogenic effect of CDNR on bone cells. (a) UMR106 proliferation at different concentration of CDNR assessed by BrdU assay; (b) UMR106 viability at different concentration of CDNR assessed by MTT assay. (CDNR(aq), CDNR(e)) Aqueous and ethanol component of CDNR. Data are expressed as mean and standard deviation (error bar). **P < 0.01, ***P < 0.001 versus Control (0 μg/mL).
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fig2: Osteogenic effect of CDNR on bone cells. (a) UMR106 proliferation at different concentration of CDNR assessed by BrdU assay; (b) UMR106 viability at different concentration of CDNR assessed by MTT assay. (CDNR(aq), CDNR(e)) Aqueous and ethanol component of CDNR. Data are expressed as mean and standard deviation (error bar). **P < 0.01, ***P < 0.001 versus Control (0 μg/mL).

Mentions: As shown in Figure 2(a), after treatment of CDNR(aq) for 24 h, significant proliferative effects were observed in UMR-106 cells from 25 to 100 μg/mL (P < 0.001) with increments of 10.8% to 22%. Similar significant cell viability-enhancement effects were observed in UMR-106 from 6.25 to 100 μg/mL (Figure 2(b)). CDNR(e) did not affect UMR-106 cells after 24 h of treatment and up to 100 μg/mL in both assays.


Evaluation of a topical herbal agent for the promotion of bone healing.

Siu WS, Ko CH, Lam KW, Wat E, Shum WT, Lau CB, Ko KM, Hung LK, Lau DT, Leung PC - Evid Based Complement Alternat Med (2015)

Osteogenic effect of CDNR on bone cells. (a) UMR106 proliferation at different concentration of CDNR assessed by BrdU assay; (b) UMR106 viability at different concentration of CDNR assessed by MTT assay. (CDNR(aq), CDNR(e)) Aqueous and ethanol component of CDNR. Data are expressed as mean and standard deviation (error bar). **P < 0.01, ***P < 0.001 versus Control (0 μg/mL).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4355818&req=5

fig2: Osteogenic effect of CDNR on bone cells. (a) UMR106 proliferation at different concentration of CDNR assessed by BrdU assay; (b) UMR106 viability at different concentration of CDNR assessed by MTT assay. (CDNR(aq), CDNR(e)) Aqueous and ethanol component of CDNR. Data are expressed as mean and standard deviation (error bar). **P < 0.01, ***P < 0.001 versus Control (0 μg/mL).
Mentions: As shown in Figure 2(a), after treatment of CDNR(aq) for 24 h, significant proliferative effects were observed in UMR-106 cells from 25 to 100 μg/mL (P < 0.001) with increments of 10.8% to 22%. Similar significant cell viability-enhancement effects were observed in UMR-106 from 6.25 to 100 μg/mL (Figure 2(b)). CDNR(e) did not affect UMR-106 cells after 24 h of treatment and up to 100 μg/mL in both assays.

Bottom Line: This indicated its anti-inflammatory effect.However, CDNR did not affect the bone turnover markers in serum of the rats.With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chinese Medicine, 5/F, The CUHK Hong Kong Jockey Club School of Public Health Building, Prince of Wales Hospital, Shatin, New Territories, Hong Kong ; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong ; Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

ABSTRACT
A topically used Chinese herbal paste, namely, CDNR, was designed to facilitate fracture healing which is usually not addressed in general hospital care. From our in vitro studies, CDNR significantly inhibited the release of nitric oxide from RAW264.7 cells by 51 to 77%. This indicated its anti-inflammatory effect. CDNR also promoted the growth of bone cells by stimulating the proliferation of UMR106 cells up to 18%. It also increased the biomechanical strength of the healing bone in a drill-hole defect rat model by 16.5% significantly. This result revealed its in vivo efficacy on facilitation of bone healing. Furthermore, the detection of the chemical markers of CDNR in the skin and muscle of the treatment area demonstrated its transdermal properties. However, CDNR did not affect the bone turnover markers in serum of the rats. With its anti-inflammatory and bone formation properties, CDNR is found effective in promoting bone healing.

No MeSH data available.


Related in: MedlinePlus