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Bone morphology in 46 BXD recombinant inbred strains and femur-tibia correlation.

Zhang Y, Huang J, Jiao Y, David V, Kocak M, Roan E, Di'Angelo D, Lu L, Hasty KA, Gu W - ScientificWorldJournal (2015)

Bottom Line: While genetics and gender appear expectedly as the major determinants of bone mass and structure, significant correlations were also observed between femur and tibia.More importantly, positive and negative femur-tibia associations indicated that genetic makeup had an influence on skeletal integrity.We conclude that (a) femur-tibia association in bone morphological properties significantly varies from strain to strain, which may be caused by genetic differences among strains, and (b) strainwise variations were seen in bone mass, bone morphology, and bone microarchitecture along with bone structural property.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery and Biomedical Engineering, Campbell Clinic, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

ABSTRACT
We examined the bone properties of BXD recombinant inbred (RI) mice by analyzing femur and tibia and compared their phenotypes of different compartments. 46 BXD RI mouse strains were analyzed including progenitor C57BL/6J (n = 16) and DBA/2J (n = 15) and two first filial generations (D2B6F1 and B6D2F1). Strain differences were observed in bone quality and structural properties (P < 0.05) in each bone profile (whole bone, cortical bone, or trabecular bone). It is well known that skeletal phenotypes are largely affected by genetic determinants and genders, such as bone mineral density (BMD). While genetics and gender appear expectedly as the major determinants of bone mass and structure, significant correlations were also observed between femur and tibia. More importantly, positive and negative femur-tibia associations indicated that genetic makeup had an influence on skeletal integrity. We conclude that (a) femur-tibia association in bone morphological properties significantly varies from strain to strain, which may be caused by genetic differences among strains, and (b) strainwise variations were seen in bone mass, bone morphology, and bone microarchitecture along with bone structural property.

No MeSH data available.


Related in: MedlinePlus

Spearman's rank correlations in RI Strain BXD95 whole bone profile showed a variety of associations between femur and tibia. (a) r = 1.0, P < 0.0001; (b) r = 1.0, P < 0.0001; (c) r = 0.9, P = 0.04.
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fig3: Spearman's rank correlations in RI Strain BXD95 whole bone profile showed a variety of associations between femur and tibia. (a) r = 1.0, P < 0.0001; (b) r = 1.0, P < 0.0001; (c) r = 0.9, P = 0.04.

Mentions: For trabecular bone analysis, a region of 100 transverse slices at the secondary spongiosa in distal femur or distal tibia site was measured (Figure 3). The bone volume fraction was calculated directly by plotting gray voxels representing bone fraction against gray plus black voxel (nonbone objects; VOX BV/TV). Bone surface (BS) was calculated using a tetrahedron meshing technique generated by the “marching cubes method” and total volume (TV) was taken as the volume of interest (VOI). The normalized indices (BV/TV, BS/BV, and BS/BV) were used [8].


Bone morphology in 46 BXD recombinant inbred strains and femur-tibia correlation.

Zhang Y, Huang J, Jiao Y, David V, Kocak M, Roan E, Di'Angelo D, Lu L, Hasty KA, Gu W - ScientificWorldJournal (2015)

Spearman's rank correlations in RI Strain BXD95 whole bone profile showed a variety of associations between femur and tibia. (a) r = 1.0, P < 0.0001; (b) r = 1.0, P < 0.0001; (c) r = 0.9, P = 0.04.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4355808&req=5

fig3: Spearman's rank correlations in RI Strain BXD95 whole bone profile showed a variety of associations between femur and tibia. (a) r = 1.0, P < 0.0001; (b) r = 1.0, P < 0.0001; (c) r = 0.9, P = 0.04.
Mentions: For trabecular bone analysis, a region of 100 transverse slices at the secondary spongiosa in distal femur or distal tibia site was measured (Figure 3). The bone volume fraction was calculated directly by plotting gray voxels representing bone fraction against gray plus black voxel (nonbone objects; VOX BV/TV). Bone surface (BS) was calculated using a tetrahedron meshing technique generated by the “marching cubes method” and total volume (TV) was taken as the volume of interest (VOI). The normalized indices (BV/TV, BS/BV, and BS/BV) were used [8].

Bottom Line: While genetics and gender appear expectedly as the major determinants of bone mass and structure, significant correlations were also observed between femur and tibia.More importantly, positive and negative femur-tibia associations indicated that genetic makeup had an influence on skeletal integrity.We conclude that (a) femur-tibia association in bone morphological properties significantly varies from strain to strain, which may be caused by genetic differences among strains, and (b) strainwise variations were seen in bone mass, bone morphology, and bone microarchitecture along with bone structural property.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery and Biomedical Engineering, Campbell Clinic, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

ABSTRACT
We examined the bone properties of BXD recombinant inbred (RI) mice by analyzing femur and tibia and compared their phenotypes of different compartments. 46 BXD RI mouse strains were analyzed including progenitor C57BL/6J (n = 16) and DBA/2J (n = 15) and two first filial generations (D2B6F1 and B6D2F1). Strain differences were observed in bone quality and structural properties (P < 0.05) in each bone profile (whole bone, cortical bone, or trabecular bone). It is well known that skeletal phenotypes are largely affected by genetic determinants and genders, such as bone mineral density (BMD). While genetics and gender appear expectedly as the major determinants of bone mass and structure, significant correlations were also observed between femur and tibia. More importantly, positive and negative femur-tibia associations indicated that genetic makeup had an influence on skeletal integrity. We conclude that (a) femur-tibia association in bone morphological properties significantly varies from strain to strain, which may be caused by genetic differences among strains, and (b) strainwise variations were seen in bone mass, bone morphology, and bone microarchitecture along with bone structural property.

No MeSH data available.


Related in: MedlinePlus