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Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.

Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T - Sci Rep (2015)

Bottom Line: Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process.The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process.In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan.

ABSTRACT
Block-face imaging is a scanning electron microscopic technique which enables easier acquisition of serial ultrastructural images directly from the surface of resin-embedded biological samples with a similar quality to transmission electron micrographs. In the present study, we analyzed the three-dimensional architecture of podocytes using serial block-face imaging. It was previously believed that podocytes are divided into three kinds of subcellular compartment: cell body, primary process, and foot process, which are simply aligned in this order. When the reconstructed podocytes were viewed from their basal side, the foot processes were branched from a ridge-like prominence, which was formed on the basal surface of the primary process and was similar to the usual foot processes in structure. Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process. The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process. In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

No MeSH data available.


Related in: MedlinePlus

Structural relation of ridge-like prominence and foot processes.(a) Luminal surface view of podocytes shows primary and foot processes. Cross sections at the sites indicated by arrows (Sec. b, c, d) are shown in (b, c, d), respectively. (b) Podocytes adhere to the glomerular basement membrane with their foot processes. (c, d) The basal portion of primary process form the ridge-like prominence, and the primary process adheres to the glomerular basement membrane via the ridge-like prominence (RLP). The foot processes also protrude from the primary process via the ridge-like prominence. F, foot process; GBM, glomerular basement membrane; P, primary process.
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f6: Structural relation of ridge-like prominence and foot processes.(a) Luminal surface view of podocytes shows primary and foot processes. Cross sections at the sites indicated by arrows (Sec. b, c, d) are shown in (b, c, d), respectively. (b) Podocytes adhere to the glomerular basement membrane with their foot processes. (c, d) The basal portion of primary process form the ridge-like prominence, and the primary process adheres to the glomerular basement membrane via the ridge-like prominence (RLP). The foot processes also protrude from the primary process via the ridge-like prominence. F, foot process; GBM, glomerular basement membrane; P, primary process.

Mentions: To reveal the structural relationship among the three subcellular compartments of podocytes, we next observed single reconstructed podocyte which was separated from neighboring podocytes (Fig. 5, Movies S1, S2). When viewed from the basal side of the primary processes, we found that the foot processes were branched from a ridge-like prominence, which was protruded from the basal surface of the primary process (Fig. 5b1, 5b2) and was directly attached to the GBM like the foot processes (Supplementary Fig. S2). The distal ends of foot processes made their way under the primary process, and connected to the lateral surface of the ridge-like prominence via the slit diaphragm (Figs. 3b, 3b′, 6).


Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.

Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T - Sci Rep (2015)

Structural relation of ridge-like prominence and foot processes.(a) Luminal surface view of podocytes shows primary and foot processes. Cross sections at the sites indicated by arrows (Sec. b, c, d) are shown in (b, c, d), respectively. (b) Podocytes adhere to the glomerular basement membrane with their foot processes. (c, d) The basal portion of primary process form the ridge-like prominence, and the primary process adheres to the glomerular basement membrane via the ridge-like prominence (RLP). The foot processes also protrude from the primary process via the ridge-like prominence. F, foot process; GBM, glomerular basement membrane; P, primary process.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355681&req=5

f6: Structural relation of ridge-like prominence and foot processes.(a) Luminal surface view of podocytes shows primary and foot processes. Cross sections at the sites indicated by arrows (Sec. b, c, d) are shown in (b, c, d), respectively. (b) Podocytes adhere to the glomerular basement membrane with their foot processes. (c, d) The basal portion of primary process form the ridge-like prominence, and the primary process adheres to the glomerular basement membrane via the ridge-like prominence (RLP). The foot processes also protrude from the primary process via the ridge-like prominence. F, foot process; GBM, glomerular basement membrane; P, primary process.
Mentions: To reveal the structural relationship among the three subcellular compartments of podocytes, we next observed single reconstructed podocyte which was separated from neighboring podocytes (Fig. 5, Movies S1, S2). When viewed from the basal side of the primary processes, we found that the foot processes were branched from a ridge-like prominence, which was protruded from the basal surface of the primary process (Fig. 5b1, 5b2) and was directly attached to the GBM like the foot processes (Supplementary Fig. S2). The distal ends of foot processes made their way under the primary process, and connected to the lateral surface of the ridge-like prominence via the slit diaphragm (Figs. 3b, 3b′, 6).

Bottom Line: Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process.The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process.In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan.

ABSTRACT
Block-face imaging is a scanning electron microscopic technique which enables easier acquisition of serial ultrastructural images directly from the surface of resin-embedded biological samples with a similar quality to transmission electron micrographs. In the present study, we analyzed the three-dimensional architecture of podocytes using serial block-face imaging. It was previously believed that podocytes are divided into three kinds of subcellular compartment: cell body, primary process, and foot process, which are simply aligned in this order. When the reconstructed podocytes were viewed from their basal side, the foot processes were branched from a ridge-like prominence, which was formed on the basal surface of the primary process and was similar to the usual foot processes in structure. Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process. The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process. In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

No MeSH data available.


Related in: MedlinePlus