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Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.

Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T - Sci Rep (2015)

Bottom Line: Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process.The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process.In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan.

ABSTRACT
Block-face imaging is a scanning electron microscopic technique which enables easier acquisition of serial ultrastructural images directly from the surface of resin-embedded biological samples with a similar quality to transmission electron micrographs. In the present study, we analyzed the three-dimensional architecture of podocytes using serial block-face imaging. It was previously believed that podocytes are divided into three kinds of subcellular compartment: cell body, primary process, and foot process, which are simply aligned in this order. When the reconstructed podocytes were viewed from their basal side, the foot processes were branched from a ridge-like prominence, which was formed on the basal surface of the primary process and was similar to the usual foot processes in structure. Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process. The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process. In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

No MeSH data available.


Related in: MedlinePlus

Podocyte subcellular compartments shown by conventional SEM and TEM.Podocyte are traditionally divided into three kinds of subcellular compartment, cell body (CB), primary process (P) and foot process. (a) Conventional SEM image shows the luminal surface of all three kinds of podocyte subcellular compartments. Three neighboring podocytes are individually colored with blue, green, and red. (b, c) Conventional TEM images also show the three subcellular compartments. The foot processes are predominantly protruding from the primary processes, but some of them are from the undersurface of cell body (yellow arrows in b). The foot processes possess the electron-dense actin bundle within their luminal cytoplasm (yellow arrowheads in c). Directions of luminal and basal views are indicated by black arrows. (d) Alkaline-maceration SEM images clearly show the basal surface of neighboring podocytes. The region in contact with the glomerular basement membrane consists almost entirely of the interdigitating foot processes. Two neighboring podocytes are individually colored with green and red. Cap, capillary lumen. US, urinary space of the Bowman's capsule. Bar scales, 1 μm in (a), (b); 200 nm in (c), (d).
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f1: Podocyte subcellular compartments shown by conventional SEM and TEM.Podocyte are traditionally divided into three kinds of subcellular compartment, cell body (CB), primary process (P) and foot process. (a) Conventional SEM image shows the luminal surface of all three kinds of podocyte subcellular compartments. Three neighboring podocytes are individually colored with blue, green, and red. (b, c) Conventional TEM images also show the three subcellular compartments. The foot processes are predominantly protruding from the primary processes, but some of them are from the undersurface of cell body (yellow arrows in b). The foot processes possess the electron-dense actin bundle within their luminal cytoplasm (yellow arrowheads in c). Directions of luminal and basal views are indicated by black arrows. (d) Alkaline-maceration SEM images clearly show the basal surface of neighboring podocytes. The region in contact with the glomerular basement membrane consists almost entirely of the interdigitating foot processes. Two neighboring podocytes are individually colored with green and red. Cap, capillary lumen. US, urinary space of the Bowman's capsule. Bar scales, 1 μm in (a), (b); 200 nm in (c), (d).

Mentions: Podocytes develop a characteristic architecture specialized for glomerular ultrafiltration, and are traditionally divided into three kinds of subcellular compartment; the cell body, primary process, and foot process1. The cell body of podocyte possesses several thick primary processes, each of which subsequently projects a number of fine foot processes (Fig. 1a, b). Adjacent podocytes are interdigitated with each other at their foot processes, which are separated from each other by filtration slits and bridged with a specialized intercellular junction, called a slit diaphragm (Fig. 1c). The foot processes and slit diaphragm serve as an adhesive apparatus to the glomerular basement membrane (GBM) and as a filtration barrier together with GBM and endothelial glycocalyx, respectively.


Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.

Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T - Sci Rep (2015)

Podocyte subcellular compartments shown by conventional SEM and TEM.Podocyte are traditionally divided into three kinds of subcellular compartment, cell body (CB), primary process (P) and foot process. (a) Conventional SEM image shows the luminal surface of all three kinds of podocyte subcellular compartments. Three neighboring podocytes are individually colored with blue, green, and red. (b, c) Conventional TEM images also show the three subcellular compartments. The foot processes are predominantly protruding from the primary processes, but some of them are from the undersurface of cell body (yellow arrows in b). The foot processes possess the electron-dense actin bundle within their luminal cytoplasm (yellow arrowheads in c). Directions of luminal and basal views are indicated by black arrows. (d) Alkaline-maceration SEM images clearly show the basal surface of neighboring podocytes. The region in contact with the glomerular basement membrane consists almost entirely of the interdigitating foot processes. Two neighboring podocytes are individually colored with green and red. Cap, capillary lumen. US, urinary space of the Bowman's capsule. Bar scales, 1 μm in (a), (b); 200 nm in (c), (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355681&req=5

f1: Podocyte subcellular compartments shown by conventional SEM and TEM.Podocyte are traditionally divided into three kinds of subcellular compartment, cell body (CB), primary process (P) and foot process. (a) Conventional SEM image shows the luminal surface of all three kinds of podocyte subcellular compartments. Three neighboring podocytes are individually colored with blue, green, and red. (b, c) Conventional TEM images also show the three subcellular compartments. The foot processes are predominantly protruding from the primary processes, but some of them are from the undersurface of cell body (yellow arrows in b). The foot processes possess the electron-dense actin bundle within their luminal cytoplasm (yellow arrowheads in c). Directions of luminal and basal views are indicated by black arrows. (d) Alkaline-maceration SEM images clearly show the basal surface of neighboring podocytes. The region in contact with the glomerular basement membrane consists almost entirely of the interdigitating foot processes. Two neighboring podocytes are individually colored with green and red. Cap, capillary lumen. US, urinary space of the Bowman's capsule. Bar scales, 1 μm in (a), (b); 200 nm in (c), (d).
Mentions: Podocytes develop a characteristic architecture specialized for glomerular ultrafiltration, and are traditionally divided into three kinds of subcellular compartment; the cell body, primary process, and foot process1. The cell body of podocyte possesses several thick primary processes, each of which subsequently projects a number of fine foot processes (Fig. 1a, b). Adjacent podocytes are interdigitated with each other at their foot processes, which are separated from each other by filtration slits and bridged with a specialized intercellular junction, called a slit diaphragm (Fig. 1c). The foot processes and slit diaphragm serve as an adhesive apparatus to the glomerular basement membrane (GBM) and as a filtration barrier together with GBM and endothelial glycocalyx, respectively.

Bottom Line: Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process.The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process.In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan.

ABSTRACT
Block-face imaging is a scanning electron microscopic technique which enables easier acquisition of serial ultrastructural images directly from the surface of resin-embedded biological samples with a similar quality to transmission electron micrographs. In the present study, we analyzed the three-dimensional architecture of podocytes using serial block-face imaging. It was previously believed that podocytes are divided into three kinds of subcellular compartment: cell body, primary process, and foot process, which are simply aligned in this order. When the reconstructed podocytes were viewed from their basal side, the foot processes were branched from a ridge-like prominence, which was formed on the basal surface of the primary process and was similar to the usual foot processes in structure. Moreover, from the cell body, the foot processes were also emerged via the ridge-like prominence, as found in the primary process. The ridge-like prominence anchored the cell body and primary process to the glomerular basement membrane, and connected the foot processes to the cell body and primary process. In conclusion, serial block-face imaging is a powerful tool for clear understanding the three-dimensional architecture of podocytes through its ability to reveal novel structures which were difficult to determine by conventional transmission and scanning electron microscopes alone.

No MeSH data available.


Related in: MedlinePlus